Killer immune cell culturing method and application thereof
A technology of immune cells and culture methods, applied in the direction of cell culture active agents, blood/immune system cells, animal cells, etc., can solve the problems of low efficacy and low specificity, and achieve enhanced function, enhanced killing, and strong tumor killing The effect of cellular capacity
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[0023] Add DC cell activation factors GM-CSF and IL-4, and multiple tumor antigen proteins or long-chain polypeptides (0.1ug / ml / each) to the medium for initially culturing PBMCs, place at 37°C, 5% CO 2 Culture in the incubator for 12-16 hours, add DC cell activation factor IFN-γ, CpG, Poly (I:C), R848, melanoma tumor antigen short peptide (0.1ug / ml), place at 37°C, 5% CO 2 Cultured in an incubator, the cells were harvested after 12 days of continuous culture.
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