Method for detecting expression of microRNA-126-5p in myocardial damage

A technology of myocardial injury and detection method, which is applied in the field of clinical medicine, can solve the problems that the expression detection method is not mature enough, the detection result is not intuitive enough, etc.

Inactive Publication Date: 2016-06-22
CENT SOUTH UNIV
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  • Description
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Problems solved by technology

[0004] However, the detection method for microRNA expression in the prior art is not mature enough, and the detection results are not intuitive enough

Method used

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Embodiment Construction

[0034] The present invention will be further described below in conjunction with embodiment.

[0035] The method for detecting the expression of microRNA-126-5p in myocardial injury comprises the following steps:

[0036] S1. Take out the collected myocardial tissue samples from -80°C, and use Trizol to extract RNA from the tissue;

[0037] S2. Reverse transcribe RNA into cDNA: Prepare a reaction solution according to the reverse transcription reagent (the preparation of the reaction solution is carried out on ice), dilute the reaction solution, take the diluted solution and add it to the next step of the RealTimePCR reaction system for quantitative detection;

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Abstract

The invention relates to a method for detecting expression of microRNA-126-5p in myocardial damage. The method includes following steps: S1, taking out a myocardial tissue specimen collected at minus 80 DEG C, and using Trizol to extract RNA in tissue; S2, reversely transcribing RNA into cDNA: preparing reaction liquid according to a reverse transcription reagent, diluting the reaction liquid, taking and adding diluent into a Real Time PCR system in the next step, and performing quantitative detection; S3, performing RT-QPCR quantitative detection: preparing PCR reaction liquid, and performing Real time PCR, namely S3.1, pre-modifying DNA at 95 DEG C for 30 S, S3.2, performing PCR at 95 DEG C for 5 S and at 60 DEG C for 34 S, and repeating 40 times. The method has the advantage that expression of microRNA-126-5p in myocardial damage can be detected quickly.

Description

technical field [0001] The invention relates to the field of clinical medicine, in particular to a method for detecting the expression of microRNA-126-5p in myocardial injury. Background technique [0002] MicroRNA is an endogenous single-stranded non-coding RNA discovered in recent years, consisting of 18-25 nucleotides, and a single-stranded RNA precursor with a hairpin loop structure of 70-80 nucleotides in length Generated after cutting. There are approximately 1000 microRNAs detected in the human genome. The expression of microRNAs is significantly changed under various pathological stress conditions, fully demonstrating their role in various diseases. [0003] In recent years, with the discovery of microRNA, the research on myocardial ischemic injury and protection has been further advanced. Researchers have found that microRNA plays an important role in myocardial ischemic injury. [0004] However, the detection method for microRNA expression in the prior art is no...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6883C12Q2600/172C12Q2600/158
Inventor 蒋碧梅梁鹏飞肖献忠
Owner CENT SOUTH UNIV
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