Anti-sheeppox virus K3L protein monoclonal antibody and application thereof
A monoclonal antibody, sheep pox virus technology, applied to hybridoma cells or antibodies in the preparation of detection or detection of sheep pox virus reagents, the field of monoclonal antibodies against the whole protein of sheep pox virus K3L, can solve the economic problem of sheep farming industry loss, etc.
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[0030] The present invention is illustrated below in conjunction with examples. The following content is only used to illustrate the relevant details of the present invention, and should not be interpreted as limiting the protection scope of the present invention.
[0031] 1. Strains and Cells
[0032] The virus strain used in the present invention is the Gulang strain of sheep pox virus, and the used virus strain, Vero cells and SP2 / 0 cells are all preserved by the applicant (Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences).
[0033] 2. Main reagents
[0034]The pUC57 cloning vector, Top10 competent cells, plasmid extraction kit, XbaⅠ and BamHI restriction endonucleases, and T4 DNA ligase were purchased from Dalian Bao Biological Engineering Co., Ltd.; DMEM medium, RPMI-1640 cell culture medium and fetal bovine serum All were purchased from Gibco Company; gel recovery kit was purchased from AXYGEN Company; pET11d expression vector, BL21(DE3) ...
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