Anti-sheeppox virus K3L protein monoclonal antibody and application thereof

A monoclonal antibody, sheep pox virus technology, applied to hybridoma cells or antibodies in the preparation of detection or detection of sheep pox virus reagents, the field of monoclonal antibodies against the whole protein of sheep pox virus K3L, can solve the economic problem of sheep farming industry loss, etc.

Active Publication Date: 2016-07-13
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Sheeppoxvirus (SPV) belongs to Poxviridae, Chordatepoxvirinae, and Goatpoxvirus genus. It is mainly transmitted through aerosol, close contact or insect bites. Sheep is the main host of sheep poxvirus. All can be infected, but the death cases mainly occur in lambs, and the mortality rate is as high as 100%. It has caused significant economic losses to the sheep industry and is considered to be an economically potential biological weapon.

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  • Anti-sheeppox virus K3L protein monoclonal antibody and application thereof
  • Anti-sheeppox virus K3L protein monoclonal antibody and application thereof
  • Anti-sheeppox virus K3L protein monoclonal antibody and application thereof

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Embodiment Construction

[0030] The present invention is illustrated below in conjunction with examples. The following content is only used to illustrate the relevant details of the present invention, and should not be interpreted as limiting the protection scope of the present invention.

[0031] 1. Strains and Cells

[0032] The virus strain used in the present invention is the Gulang strain of sheep pox virus, and the used virus strain, Vero cells and SP2 / 0 cells are all preserved by the applicant (Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences).

[0033] 2. Main reagents

[0034]The pUC57 cloning vector, Top10 competent cells, plasmid extraction kit, XbaⅠ and BamHI restriction endonucleases, and T4 DNA ligase were purchased from Dalian Bao Biological Engineering Co., Ltd.; DMEM medium, RPMI-1640 cell culture medium and fetal bovine serum All were purchased from Gibco Company; gel recovery kit was purchased from AXYGEN Company; pET11d expression vector, BL21(DE3) ...

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Abstract

The invention discloses a hybridoma cell line K3L25. The preservation number of the hybridoma cell line K3L25 in China Center for Type Culture Collection (CCTCC) is CCTCC NO: C2015222. The hybridoma cell line K3L25 and the monoclonal antibody secreted by the hybridoma cell line K3L25 show good immunogenicity, and can be applied in preparation of an early diagnosis reagent or a reagent for early diagnosing sheeppox virus infection or a reagent for basic tests.

Description

technical field [0001] The present invention relates to a monoclonal antibody, specifically the present invention relates to a hybridoma cell line against the K3L protein of sheeppox virus and the monoclonal antibody secreted by it against the whole protein of sheeppox virus K3L, and the hybridoma or Application of the antibody in the preparation of a reagent for detection or detection of sheep pox virus. Background technique [0002] Goat pox is a highly contagious and devastating viral disease among sheep epidemics. It is listed as a legally notifiable animal disease by the World Organization for Animal Health (OIE). Difficulty and death are the hallmarks. Goat pox is mainly prevalent in some areas of Africa, the Middle East, India, Nepal and Turkey, and it is mainly prevalent in Qinghai, Gansu, Hunan and Inner Mongolia in China. Sheeppoxvirus (SPV) belongs to Poxviridae, Chordatepoxvirinae, and Goatpoxvirus genus. It is mainly transmitted through aerosol, close contact ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/20C07K16/08G01N33/577G01N33/569C12R1/91
CPCC07K16/081C12N5/163
Inventor 赵志荀朱学亮张强张志东吴国华颜新敏李健朱海霞吴娜
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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