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Method for tissue culture and rapid propagation of Urena procumbens Linn.

A technology of tissue culture and smallpox, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of inability to obtain rapid reproduction, etc., and achieve the effect that is conducive to industrial production

Active Publication Date: 2016-08-17
益福隆(磐安)酒业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In our country, at present, peach blossoms are mainly propagated by seeds, which are limited by the number of seeds, germination rate and climate, so they cannot be reproduced rapidly.

Method used

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  • Method for tissue culture and rapid propagation of Urena procumbens Linn.
  • Method for tissue culture and rapid propagation of Urena procumbens Linn.
  • Method for tissue culture and rapid propagation of Urena procumbens Linn.

Examples

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Embodiment 1

[0030] The present embodiment provides a kind of Brahmanthus flower tissue culture and the method for rapid propagation, comprise the following steps:

[0031] (1) Disinfection of Brahma flower

[0032] Cut off the stems with leaves that were pulled out in the same year, first use a soft brush to gently scrub under running water, cut off the leaves, leaving only a small amount of petiole, put it in a container with a small amount of detergent, stir and soak for 2-4 minutes, pour it out After rinsing with running water for 0.5 hours, put the detergent solution into an ultra-clean bench that has been sterilized by ultraviolet light for more than 30 minutes, put the stem section into 70% alcohol solution and soak for 40 seconds, rinse it with sterile water for 3 times, and immerse it in a dropwise solution with Soak and disinfect 1 drop of Tween-80 and 1 drop of 1mol / L NaOH solution in 0.1% HgCl2 solution for 11-12min, then fully soak with sterile water for 3 times, that is the s...

Embodiment 2

[0050] The difference between the present embodiment and Example 1 is that step (2): the stem segment explant obtained in step (1) is cut off the two ends that a small amount of disinfectant contacts with a scalpel that burns and cools on sterile wet filter paper, and Inoculate in the direction of normal polarity into 1 / 2 MS basic medium supplemented with 6-BA 0-5mg / L, IBA 0-1.0mg / L, AC 1.0g / L, the macroelement content of this medium is MS basic 1 / 2 of the medium, the content of trace elements, iron salts, organic substances, inositol, etc. is the same as that of MS basic medium, the amount of sucrose added in this medium is 30.0g / L, and the amount of agar added is 8.0g / L , pH is 5.8-6.0, the culture medium was sterilized under high temperature and high pressure at 121°C for 20min; the culture medium of the explants was first placed in the dark overnight, and then cultured under light, the culture conditions were: the culture temperature was (25±1 )°C, the light time was 14h l...

Embodiment 3

[0054] The difference between this embodiment and Example 1 is that step (3): the induction of clustered teeth: the adventitious buds germinated from the axillary buds obtained in step (2) are cut into stem segments with one leaf, and inoculated to the added In the 1 / 2MS basic medium with ZT 0-1.0mg / L, 6-BA 0-5mg / L, IBA0-1.0mg / L, CH 0.5g / L, AC 0.5-1.0g / L, the medium The content of macroelements is 1 / 2 of that of MS basic medium, and the contents of trace elements, iron salts, organic substances, inositol, etc. are the same as those of MS basic medium. The volume is 8.0g / L, the pH is 5.8-6.0, and the culture medium has been sterilized under high temperature and high pressure at 121°C for 20min; The temperature is (25±1)℃, the light time is 14h light / 10h dark, and the light intensity is 30-40μmol / (m2 s); after 25 days of culture, it is found that clustered buds grow with the increase of ZT and 6-BA content. The number of adventitious buds increases with the continuous increase ...

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Abstract

The invention belongs to the technical field of plant culture and relates to a method for tissue culture and rapid propagation of Urena procumbens Linn.. The method comprises the following steps: disinfecting procumbent urena, inducing germination of adventitious buds from axillary buds, inducing cluster buds, propagating the adventitious buds, carrying out rooting culture of regenerated plants, and acclimatizing and transplanting. The tissue culture and rapid propagation method can be used for rapidly obtaining urena lobata plants with the properties the same as those of mother plants without limitation of the quantity and germination rate of urena lobata seeds and weather, thereby facilitating industrial production.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and relates to a method for tissue culture and rapid propagation of Brahmania japonica. Background technique [0002] Urena procumbens Linn. is a small deciduous shrub of the Malvaceae genus Urena, produced in Guangdong, Taiwan, Fujian, Guangxi, Jiangxi, Hunan, Zhejiang and other provinces and regions. Often grows in small shrubs on hillsides. Its whole herb is used as medicine named Brahma flower "Fujian folk herbal medicine", different names Triangle maple, Sanhe maple "Plant Name and Reality Catalog", Hong Kong wild cotton, dog wrestling trace, wild eggplant, rags, fake meat flower, lice Matou "Fujian Folk Herbal Medicine", Wulonghui, sticky flower clothes, fake cotton "Mindong Materia Medica", ground cotton, eight big hammers, thousand hammers "Jiangxi Herbal Medicine", small peach blossom (Guangzhou Army "Common Chinese Herbal Medicine Handbook"), small "Guangxi Herbs", Wild Cott...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001A01H4/008
Inventor 熊耀康张春椿孙骏威张水利俞冰李石清
Owner 益福隆(磐安)酒业有限公司
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