Urethra prosthesis reconstructing method based on 3D printing forming

Abstract

The invention discloses a urethra prosthesis reconstructing method based on 3D printing forming. The method comprises the steps that adipose-derived stem cells are differentiated into epithelioid cells and smooth muscle-like cells through cell inducing factors and then converted into cell liquid drops, and the cell liquid drops are prepared by mixing the cells with culture liquid hydrogel and prepared into biological ink capable of being used by a 3D printer; hydrogel prepared by mixing collagen with alginate is selected as stent ink of a stent material; the cells or cell clusters, the proportion of the cells to the gel and the jetting positions and strength of nozzles are controlled by a computer, two spray heads are controlled by an electronically-controlled lifting platform to go up and down, and the urethra section is alternately printed by the two spray heads of the 3D printer layer by layer. According to the method, the adipose-derived stem cells are cultured in vitro, induced to be differentiated into the urothelial cells and the smooth muscle cells and then converted into the cell liquid drops, the urethra is reconstructed in vitro by combining 3D printing, then the reconstructed urethra is transplanted into the body of a patient, and therefore the urethra reconstruction problem is solved.

Description

technical field [0001] The invention relates to the preparation of a urethral prosthesis, in particular to a method for reconstructing a urethral prosthesis based on 3D printing. Background technique [0002] The etiology and condition of urethral diseases are complicated, and the incidence rate is high. The related urethral reconstruction surgery is difficult and risky, and the technical mastery of urethral reconstruction in various hospitals is not balanced. Urethral diseases that often require urethral repair or reconstruction include: urethral stricture and congenital urethral malformations. Urethral strictures have varying prevalence worldwide. The main cause of urethral stricture in my country is trauma, such as pelvic fracture, blunt perineal injury; the second most common cause of urethral stricture is urethral stricture caused by various medical procedures, such as indwelling catheterization, open prostatectomy, After urethral surgery, hypospadias. Traditional ope...

AI Technical Summary

Problems solved by technology

There are many complications such as urinary fistula and stenosis in traditional operations, and some even require two-stage surgery, which makes it difficult for patients and their families to accept

Images