Four pairs of est-ssr primers and their preparation methods and their application in the construction of the fingerprint of Sakura

Abstract

The invention discloses four pairs of EST-SSR (Expressed Sequence Tag-Simple Sequence Repeat) primers. The nucleotide sequences of the four pairs of EST-SSR primers are as shown by SEQ ID NO. 1 to SEQ ID NO. 8. Design of SSR primers is carried out by selecting EST sequences of cerasus plants; screening of the SSR primers is carried out by utilizing materials of the cerasus plants; the four pairs of EST-SSR primers can be applied to the construction of a fingerprint map of the cerasus plants; the Chinese wild cerasus plants can be quickly and accurately distinguished according to an SSR amplification result; an affine relationship among the Chinese wild cerasus plants can be correctly reflected; the four pairs of EST-SSR primers can be used in aspects of the genetic diversity analysis, the germplasm identification, the species discrimination and protection, and the like, of the wild cerasus plants in China.

Description

technical field [0001] The invention relates to EST-SSR primers and a preparation method, in particular to 4 pairs of EST-SSR primers, a preparation method thereof and an application in constructing a fingerprint of a plant of the genus Sakura. Background technique [0002] According to "Flora of China", there are about 150 species of cherry blossoms in the world, and there are about 48 species and 8 varieties in China, far exceeding Japan, North Korea, Taiwan and other countries and regions, especially the wild resources are relatively rich, but our country is from From ancient times to the present, only the cultivation of edible cherries has been paid attention to, and there are not many cultivations of ornamental cherry blossom varieties, which are far behind Japan. However, Japan is the most successful in the development and utilization of cherry blossoms at present, while the research on cherry blossoms in my country is still in its infancy, and many wild resources have...

AI Technical Summary

Problems solved by technology

[0002] According to "Flora of China", there are about 150 species of cherry blossoms in the world, and there are about 48 species and 8 varieties in China, far exceeding Japan, North Korea, Taiwan and other countries and regions, especially the wild resources are relatively rich, but our country is from From ancient times to the present, only the cultivation of edible cherries has been paid attention to, and there are not many cultivations of ornamental cherry blossoms, which are far behind Japan.

However, the development and utilization of cherry blossoms is currently the most successful in Japan, while the research on cherry blossoms in my country is still in its infancy, many wild resources have not been fully developed and utilized, and there is a lack of independent variety cultivation

At present, most domestic ornamental cherry blossoms are imported from Japan, but they are not adaptable, have a short lifespan, and are also susceptible to pests and diseases. Therefore, it is urgent to cultivate independent cherry blossom varieties with strong adaptability and resistance.

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