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A kind of proanthocyanidin extracted from fresh perilla stem and leaf and its application

A technology of proanthocyanidins and perilla, applied in organic chemistry, drug combination, respiratory diseases, etc.

Active Publication Date: 2019-04-09
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] So far, there is no report on the rapid extraction of oligomeric proanthocyanidins from fresh perilla stems and leaves using microwave extraction combined with ultrasonic assistance

Method used

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  • A kind of proanthocyanidin extracted from fresh perilla stem and leaf and its application
  • A kind of proanthocyanidin extracted from fresh perilla stem and leaf and its application
  • A kind of proanthocyanidin extracted from fresh perilla stem and leaf and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Microwave extraction combined with ultrasonic assisted rapid extraction of oligomeric proanthocyanidins from perilla stems and leaves

[0024] (1) Pick the stems and leaves of high-quality perilla with no disease damage and dryness of normal plants, wash them with clean water, and dry them to obtain pretreated fresh perilla stems and leaves; weigh 500 grams of fresh perilla stems and leaves, add 1.5 L volume concentration is 65% ethanol aqueous solution (v / v), continuously grinds 2 minutes in common grinding pulverizer (JYZ-D51 of Joyoung brand), takes out thick slurry, squeezes and filters with gauze, obtains initial extract. Get the initial extract and centrifuge at 12000rpm for 15min, absorb 1.0mL of the supernatant and dilute 3 times the volume with 0.5% methanol aqueous solution, and calculate the proanthocyanidin content 44% and extraction rate by using the vanillin-hydrochloric acid colorimetric method according to the method of Example 2 3.5%.

[002...

Embodiment 2

[0034] Example 2: Rapid extraction of oligomeric proanthocyanidins in perilla stems and leaves by microwave extraction

[0035] (1) Pick the stems and leaves of high-quality perilla with no disease damage and dryness of normal plants, wash them with clean water, and dry them to obtain pretreated fresh perilla stems and leaves; weigh 500 grams of fresh perilla stems and leaves, add 1.5 L volume concentration is 65% ethanol aqueous solution (v / v), continuously grinds 2 minutes in common grinding pulverizer (JYZ-D51 of Joyoung brand), takes out thick slurry, squeezes and filters with gauze, obtains initial extract. Get the initial extract and centrifuge at 12000rpm for 15min, absorb 1.0mL of the supernatant and dilute 3 times the volume with 0.5% methanol aqueous solution, and calculate the proanthocyanidin content 44% and extraction rate by using the vanillin-hydrochloric acid colorimetric method according to the method of Example 2 3.5%.

[0036] (2) Microwave extraction

[0...

Embodiment 3

[0038] Example 3: Ultrasonic-assisted rapid extraction of oligomeric proanthocyanidins in perilla stems and leaves

[0039] (1) Pick the stems and leaves of high-quality perilla with no disease damage and dryness of normal plants, wash them with clean water, and dry them to obtain pretreated fresh perilla stems and leaves; weigh 500 grams of fresh perilla stems and leaves, add 1.5 L volume concentration is 65% ethanol aqueous solution (v / v), continuously grinds 2 minutes in common grinding pulverizer (JYZ-D51 of Joyoung brand), takes out thick slurry, squeezes and filters with gauze, obtains initial extract. Get the initial extract and centrifuge at 12000rpm for 15min, absorb 1.0mL of the supernatant and dilute 3 times the volume with 0.5% methanol aqueous solution, and calculate the proanthocyanidin content 44% and extraction rate by using the vanillin-hydrochloric acid colorimetric method according to the method of Example 2 3.5%.

[0040] (2) Ultrasonic-assisted extraction...

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Abstract

The invention discloses procyanidine extracted from fresh perilla frutescens stems and leaves and an application thereof; a procyanidine extraction method comprises the steps: adding perilla frutescens stems and leaves after pretreatment into an ethanol aqueous solution with the volume concentration of 45-65%, grinding into slurry, then carrying out microwave and ultrasonic combined extraction, and separating and purifying, to obtain the procyanidine. The invention relates to a process for assisting rapid extraction of oligo-procyanidine from the perilla frutescens stems and leaves through combining microwave extraction and ultrasonic waves; the oligo-procyanidine product with the content of more than 76% and the extraction rate of up to 9.5% can be quickly and simply extracted from the perilla frutescens stems and leaves. The oligo-procyanidine obtained from extraction can be applied for alleviating or protecting lung epithelial cell oxidative stress damage process induced by PM2.5 respiratory exposure.

Description

(1) Technical field [0001] The invention relates to an extraction of anthocyanins, in particular to a method for rapidly extracting oligomeric proanthocyanidins from perilla stems and leaves by using microwave extraction combined with ultrasonic assistance. (2) Background technology [0002] Proanthocyanidin is a polyol compound of flavanol monomer and its polymer, which widely exists in the plant kingdom. It is polymerized from varying amounts of catechin, epicatechin, gallocatechin, epigallocatechin or gallic acid. Simple proanthocyanidins generally refer to dimers, and different isomers are often formed due to different bonding positions and configurations. Proanthocyanidins dimer is the most important and the most widely distributed in plants. In addition to dimers, proanthocyanidins also have other polymers. According to the degree of polymerization, those with a degree of polymerization less than 5 are called oligomers, and those with a degree of polymerization grea...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/62A61P11/00
CPCC07D311/62
Inventor 吴石金邱乐泉钟莉陈晶张嘉琳
Owner ZHEJIANG UNIV OF TECH