A kind of ad biomarker and detection method thereof

A technology of biomarkers and detection methods, applied in the field of AD biomarkers and their detection, can solve the problems of variation, AD biomarkers limiting detection results, etc.

Active Publication Date: 2018-09-25
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In view of the above-mentioned deficiencies in the prior art, the object of the present invention is to provide an AD biomarker and its detection method, aiming at solving the problems that the application of the existing AD biomarker is limited and the detection results vary greatly

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0039] (1) Extraction of protein in saliva

[0040] 1. Sample classification, saliva of 15 cases of AD patients and 15 cases of age-matched normal control group were marked.

[0041] 2. Centrifuge at 12000r at 4°C for 10min, and absorb the supernatant.

[0042] 3. Add 450ul supernatant to the column of 10KD ultrafiltration tube, centrifuge at 11000r for 20min, repeat 3-4 times, pay attention to centrifuge the liquid on the column.

[0043] 4. Take 200ul of 50mM Tris-Hcl ((hydroxymethyl)aminomethane) to wash, centrifuge at 11000r for 20min, repeat twice.

[0044] 5. Add 200ul 50mM Tris-Hcl to dissolve the centrifuged protein.

[0045] 6. BCA quantitative determination of protein concentration.

[0046] 7. Take out 100ug protein into a new EP tube (plastic tube), keep the rest, seal and freeze dry.

[0047] (2) Salivary protease digestion step

[0048] 1. According to the protein quantification results, add 100 μg salivary protein to a 1.5 mL centrifuge tube, use Buffer (b...

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Abstract

The invention discloses an AD biomarker and a detection method thereof. The AD biomarker comprises at least one of Protein-glutamine gamma-glutamyltransferase E, Protein S100-A12, Alph-2-macroglobulin-like protein 1, Polymeric immunoglobulin receptor, Myeloperoxidase, Peroxiredoxin-1, Protein S100-A11, Alpha-2-macroglobulin, Cystatin-C, Histone H2A, Histone H2B, Tubulin alpha-1B and Annexin A3.

Description

technical field [0001] The invention relates to the technical field of AD biomarkers, in particular to an AD biomarker and a detection method thereof. Background technique [0002] Alzheimer's disease, referred to as AD (Alzheimer's Disease), is a type of neurodegenerative disease, which mainly occurs in the elderly over 65 years old. 50%, and the annual cost of dementia prevention and treatment is as high as 3150 US dollars. AD is the second largest hidden danger next to cancer in major urban populations in Europe and the United States. In my country, the incidence rate of people over 60 years old was 1.6% from 1984 to 2004, and the total number of patients has exceeded 5 million. Among various susceptibility factors, age is the most important influencing factor for AD. Among people over 65 years old, the incidence of AD is about 5.4%, and the onset tends to be younger. The symptoms and signs of AD patients are mainly manifested as insidious onset, memory impairment, cogn...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68
CPCG01N33/6896G01N2800/2821
Inventor 王勇徐金玲李水明倪嘉缵
Owner SHENZHEN UNIV
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