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Method of quickly stabilizing spinach strong female line parent and improving hybrid seed purity

A technology of hybrid purity and spinach, applied in the biological field, can solve the problems of accelerating the breeding process and speeding up the pace of spinach hybrid breeding.

Active Publication Date: 2017-05-31
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to use the characteristics of strong female lines sensitive to high temperature to quickly and stably purify strong female line materials in a short time, accelerate the breeding process, quickly solve the bottleneck problem of spinach breeding, improve the purity of hybrids, and accelerate the production of spinach hybrids. Breeding pace to fill the gap in the existing spinach breeding technology

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1. Breeding of strong female lines:

[0025] In Yantai area in winter (about November 10-December 10), sow strong female line seeds in the parent breeding greenhouse, and raise seedlings. In the greenhouse or greenhouse, if the cumulative high temperature treatment above 30°C is given for more than 60 hours at the end of flowering stage, a small amount of pollen will appear. After selfing, the seeds are harvested to obtain the seeds of the female parent line.

[0026] 2. Hybrid seed production technology:

[0027] (1) Sowing seedlings:

[0028] Sow the seedlings of the female parental line and the male parental line received by the spring propagation according to the local custom sowing method. The male parent is required to sow 10 days earlier than the female parent, and the seedling management method adopts the local conventional method.

[0029] (2) Cultivation mode:

[0030] According to the local customary cultivation mode, the planting method can be flat bed, ...

Embodiment 2

[0035] 1. Breeding of strong female lines:

[0036] In winter in Shanghai area (about December 5-January 5 of the following year), sow strong female line seeds in the parent breeding greenhouse and raise seedlings. The seedling stage is managed as normal, and spring (about February 15-March 10) is planted In the greenhouse or greenhouse, at the end of the flowering stage, a high temperature above 30°C is given for more than 60 hours of cumulative treatment, and a small amount of pollen will appear. After selfing, the seeds are harvested to obtain the seeds of the female parent line.

[0037] 2. Hybrid seed production technology:

[0038] (1) Sowing seedlings:

[0039] Sow the seedlings of the female parental line and the male parental line received by the spring propagation according to the local custom sowing method. The male parent is required to sow 7 days earlier than the female parent, and the seedling management method adopts the local conventional method.

[0040] (2...

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Abstract

The invention provides a method of quickly stabilizing a spinach strong female line parent and improving hybrid seed purity. Particularly, according to the method, a female line material is stabilized and purified quickly in a short period by using the characteristic that a strong female line is sensitive to a high temperature; a seed breeding course is accelerated; a bottleneck problem in spinach seed breeding is quickly solved; the hybrid seed purity is improved; the seed breeding pace of spinach hybrid seeds is quickened; and a blank in the existing spinach breeding technology is filled in.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular, the invention relates to a method for rapidly stabilizing strong female parents of spinach and improving the purity of hybrids. Background technique [0002] Spinach (Spinacia oleracea L.) is an annual or biennial herbaceous plant belonging to the Chenopodiaceae family of Amaranthaceae, and is one of the important economic crops. The stability is slow, and the male plants of the female parent line need to be pulled out for hybrid seed production, which is labor-intensive and time-consuming; or self-bred seeds also appear when using hermaphrodite plants as the female parent, which affects the purity of the seeds and other problems. [0003] At present, foreign countries mainly use the female line as the female parent to breed hybrid seeds, but it is necessary to select a maintainer line corresponding to the female line to preserve the female line, and to establish a breeding area for th...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 王全华邓杰徐晨曦蔡晓锋王小丽葛晨辉
Owner SHANGHAI NORMAL UNIVERSITY
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