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Breast cancer gene detection kit

A gene detection and kit technology, which is applied in the field of breast cancer gene detection kits, can solve the problems of increased use cost, long time consumption, inconvenient use, etc., and achieves the effect of low cost, simple structure and reasonable design

Inactive Publication Date: 2017-05-31
上海爱她基因科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

People use breast cancer detection kits to detect genes. Existing breast cancer detection kits can only detect a few pathogenic SNP sites, and the rest of the detection needs to be detected in other kits, which is inconvenient to use and Increase the use cost and take a long time

Method used

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  • Breast cancer gene detection kit

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Embodiment

[0020] Blood sample total DNA extraction kit was purchased from Qiagen, USA, KAPA2G Robust HotStart DNAPolymerase, dNTP, MgCl 2 , PCR buffer, KAPA HiFi Fidelity buffer, KAPA HiFi HotstartDNA Polymerase and other related reagents were purchased from Kapa Biosystems, USA; SAP and SAP reaction buffer were purchased from Affymetrix, USA; Exonuclease 1 and Exonuclease 1 reaction buffer were purchased from NEB, USA; Purchased from Axygen Company in the United States, and the 9700 PCR instrument is a product of ABI Company in the United States. 30 cases of blood samples were selected, of which 18 cases were confirmed to have breast cancer. Total DNA was extracted with blood sample total DNA extraction kit. Each case was tested as 21 reaction tubes with a total volume of 6.5ul in each tube, including 3ul of PCR reaction solution and 1ul of primers. Each tube of primers used 3 reaction tubes, 1 Add 2.5ul of DNA to be detected in the reaction tube, add 2.5ul of positive control and neg...

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Abstract

The invention discloses a breast cancer gene detection kit, which comprises a packaging box body, a gasket and test tubes, wherein the gasket is arranged in the box body and is provided with container holes; the test tubes include primer test tubes, a PCR reaction liquid test tube, an SAP reaction liquid test tube, a CODE reaction liquid test tube, connector test tubes, a magnetic bead test tube, a positive reference substance test tube and a negative reference substance test tube. The invention also discloses a using method of the kit. With the application of the finished product (the detection kit), a purpose of simultaneously detecting all exon sequences of four breast cancer related genes can be achieved, so that a detected object can be informed a risk value of being affected by breast cancer, prevention and control measures can be adopted in advance and doctors can be guided to select right clinical treatment schemes; therefore, the detection kit is conducive to targeted treatment on patients; and the finished product, which integrates detection of all exons of related genes of a same disease therein, is more convenient to use and is lower in cost, and the finished product is more convenient and scientific for assessment of a risk of being affected by the breast cancer.

Description

technical field [0001] The invention relates to a kit, in particular to a breast cancer gene detection kit. Background technique [0002] Breast cancer is a malignant tumor that occurs in the glandular epithelial tissue of the breast. 99% of breast cancer occurs in women, and only 1% in men. The mammary gland is not an important organ to maintain the life activities of the human body. In situ breast cancer is not fatal, but because breast cancer cells lose the characteristics of normal cells, the connections between cells are loose and easy to fall off. Once they fall off, the free cancer cells can follow the blood Or lymph nodes spread throughout the body, forming metastasis, which is life-threatening. The incidence of breast cancer in the world has been rising since the late 1970s, and it is gradually becoming younger, which seriously threatens the health of women. Therefore, the reasonable prevention and treatment of breast cancer is very important. [0003] Breast canc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/686C12Q1/6886C12Q2600/156C12Q2537/143
Inventor 刘贇
Owner 上海爱她基因科技有限公司
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