A blood storage device

A technology for preserving a device and blood, which is applied in the field of clinical disease diagnosis and detection, can solve problems such as cumbersome steps for proteins, and achieve the effects of simplifying steps, shortening drying time, and improving dry blood formation time.

Active Publication Date: 2019-08-09
同昕生物技术(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to make up for the deficiencies in the above fields, the present invention provides a blood preservation device, which solves the problem of cumbersome steps for separating protein in blood, and at the same time greatly reduces the time for blood drying and improves the stability of protein

Method used

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Embodiment 1

[0029] Example 1. Preparation of blood preservation device

[0030] The present invention provides a blood preservation device. In all embodiments, it is as figure 1 It has the following structural features: including a blood filter membrane 1, a molecular weight cut-off membrane 2 and a water-absorbing layer 3; the molecular weight cut-off membrane 2 is arranged below the blood filter membrane 1, and the water-absorbing membrane 2 is arranged below the molecular weight cut-off membrane 2 Layer 3; the blood filter membrane 1 is used to separate blood cells and plasma; the material of the blood filter membrane 1 is selected from whatman903 or Fusion5 or cellulose acetate membrane; the molecular weight cut-off membrane 2 is used to cut protein or nucleic acid; The material of the molecular weight cut-off membrane 2 is selected from cellulose ester membrane or ceramic membrane; the material of the water absorption layer 3 is selected from silica gel, filter paper, and CaCl 2 Or water...

Embodiment 2

[0032] Embodiment 2. Each layer is preferred

[0033] One, the optimization of blood filter membrane

[0034] In order to compare the performance of several blood filter membranes for separating plasma and blood cells, Whatman903#, Fusion5 (GE), and cellulose acetate membranes (pore sizes of 0.22μm, 0.45μm, 0.8μm, 3μm) were selected as the first blood filter membrane. Glass fiber membrane, polyester fiber membrane; the second layer uses cellulose ester film (the molecular weight cut-off is 20000, Tongshan Biological); the third layer uses anhydrous CaCl 2 (Chinese National Medicine). Drop 0.5ml of mouse whole blood on the surface of the present invention, and then re-dissolve the material retained by the second layer of cellulose ester membrane with 0.5ml of pH 7.5 10mM PBS and then use mouse CD44 molecule (CD44) ELISA Kit to determine CD44. If CD44 A positive result indicates that the blood filter membrane can effectively filter blood cells. The results show that the above-mentio...

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Abstract

The invention discloses a blood storage device. The blood storage device provided by the invention comprises a blood filtering membrane (1), a molecular weight interception membrane (2) and a water absorbing layer (3), wherein the molecular weight interception membrane (2) is arranged below the blood filtering membrane (1); the water absorbing layer (3) is arranged below the molecular weight interception membrane (2). The blood storage device provided by the invention can effectively shorten the dried blood forming time and improve the stability of protein; the blood storage device separates out a mixture containing target protein or nucleic acid by selecting a second-layer molecular weight interception membrane with suitable interception amount according to the molecular weight of the target protein and nucleic acid; in addition, the blood storage device contains the water absorbing layer, so that the spot drying time can be greatly shortened and the stability of the protein is improved.

Description

Technical field [0001] The invention belongs to the field of clinical disease diagnosis and detection, and specifically relates to a blood preservation device. Background technique [0002] The dried blood spot (DBS) method, that is, collecting whole blood samples on cardboard, has certain advantages over traditional methods in blood sample collection methods. It requires less blood, can reduce animal use, facilitate blood sample collection, storage and transportation, and is an effective tool in the screening of rare diseases. The dried blood disc method has been used in newborn screening for phenylketonuria as early as the 1960s. With the development of tandem mass spectrometry technology, the application value of dried blood disc method in the diagnosis and screening of genetic and metabolic diseases has become increasingly prominent. [0003] From the perspective of sample preservation, dried blood tablets are more stable than whole blood, so their storage and transportation ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/28G01N1/40
CPCG01N1/28G01N1/4005G01N2001/4016
Inventor 肖智焦守恕李全吴凡
Owner 同昕生物技术(北京)有限公司
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