The invention relates to a miniature blood
detector for rapidly detecting an acute
myocardial infarction marker, and a detection method thereof. The miniature blood
detector comprises a blood sample inlet (1), a blood separator (2), a serum sample outlet (3), an
immunoadsorption reactor (4), a pH-sensitive
field effect transistor (5) and a micro-current detection circuit (6); the blood sample is injected from the sample inlet (1), and flows through a
blood separation membrane (9), serum enters the
immunoadsorption reactor (4), an acute
myocardial infarction marker
antigen in the serum specifically binds to its
antibody, and is cross-linked with an
enzyme in order to cause the change of the pH value of a reaction solution in order to change the current
signal of the pH-sensitive
field effect transistor (5), the current
signal is output by the micro-current detection circuit (6), and the concentration of the measured marker is displayed. The concentration of the acute
myocardial infarction marker is detected by using
whole blood as a biological sample, combining a membrane
separation technology with a micro
biosensor element, integrating the
blood separation membrane with the micropH-sensitive
field effect transistor, performing an electronic
enzyme-linked immunosorbent reaction and adopting current response as an output in order to quickly and easily achieve clinical detectionof acute myocardial
infarction.