TLC identification method for extracts and preparations of Baoyuan decoction and its similar prescriptions

A thin-layer identification and extraction technology, which is used in the preparation of test samples, instruments, measuring devices, etc., can solve the problems of irritation and inability to identify, achieve good separation, and is conducive to industrial production, testing, application, and testing cycle. short effect

Active Publication Date: 2022-05-27
浙江金城阜通制药有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The TLC identification method is mainly to identify the single medicinal materials according to the reference pharmacopoeia method of the medicinal flavors contained therein. The conventional TLC identification cannot identify the three medicinal materials of ginseng, astragalus and licorice at the same time, and the pre-treatment is complicated and requires ammonia treatment of ginseng, Astragalus aluminum peroxide column, etc.
In addition, in the TLC identification of ginseng and astragalus recorded in the current Chinese Pharmacopoeia, the developer contains chloroform, and in the TLC identification of licorice, the developer uses glacial acetic acid, which is harmful to the operator's eyes and nose. Has a strong stimulating effect

Method used

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  • TLC identification method for extracts and preparations of Baoyuan decoction and its similar prescriptions
  • TLC identification method for extracts and preparations of Baoyuan decoction and its similar prescriptions
  • TLC identification method for extracts and preparations of Baoyuan decoction and its similar prescriptions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] Example 1: Preparation of Baoyuan Tang-Similar Recipe Extract

[0079] 1) Preparation of liquid extract: take 300-400 parts by weight of ginseng, 600-800 parts by weight of astragalus, 100-200 parts by weight of licorice, 50-100 parts by weight of cinnamon, 100-400 parts by weight of ginger, mix, pulverize, add water to carry out Heating and refluxing for extraction twice, filtering, combining the two extracts, and concentrating under reduced pressure to obtain a liquid extract.

[0080] 2) Preparation of solid extract: take the liquid extract in 1) and freeze-dry it to obtain the solid extract of Baoyuan Decoction and the like.

Embodiment 2

[0081] Embodiment 2: Baoyuan decoction oral liquid thin layer identification method

[0082] 1) Step 1: Preparation of the test solution: take 10ml of Baoyuantang oral liquid, add 10ml of n-amyl alcohol, ultrasonically treat for 25 minutes, let stand for 10 minutes, layer, absorb the supernatant, add water and wash 3 times, each 15ml , discard the water layer, evaporate the n-amyl alcohol solution to dryness, add 1 ml of methanol to the residue to dissolve it, and use it as the test solution.

[0083]2) Step 2: Preparation of reference substance solution: take ammonium glycyrrhizinate reference substance, add methanol to make 0.5mg / ml ammonium glycyrrhizinate solution; take astragaloside IV reference substance, add methanol to make 0.5mg / ml astragaloside IV solution; Take ginsenoside Rb1, Re and Rg1 reference substance, add methanol to make 0.5mg / ml ginsenoside Rb1, Re and Rg1 mixed solution.

[0084] 3) Step 3: Preparation of developing agent solution: Take ethyl acetate, fo...

Embodiment 3

[0088] Embodiment 3: Baoyuan soup tablet thin layer identification method

[0089] 1) Step 1: Preparation of the test solution: take 2g of Baoyuantang tablets, grind them finely, add 4ml of water, and stir well; add 20ml of n-amyl alcohol, ultrasonically treat for 40 minutes, let stand for 10 minutes, layer by layer, and absorb the supernatant , Wash 4 times with water, 30 ml each time, discard the water layer, evaporate the n-amyl alcohol solution to dryness, add 1 ml of methanol to the residue to dissolve it, and use it as the test solution.

[0090] 2) Step 2: Preparation of reference substance solution: take ammonium glycyrrhizinate reference substance, add methanol to make 1mg / ml ammonium glycyrrhizinate solution; take astragaloside IV reference substance, add methanol to prepare 1mg / ml astragaloside IV solution; take ginseng Saponin Rb1, Re and Rg1 reference substance, add methanol to make 1mg / ml ginsenoside Rb1, Re and Rg1 mixed solution.

[0091] 3) Step 3: Preparatio...

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Abstract

The disclosure relates to a TLC identification method for extracts and preparations of Baoyuan Decoction and its similar prescriptions, which can simultaneously identify the main medicinal flavors of ginseng, astragalus, and licorice in the extracts and preparations of Baoyuan Decoction and its similar prescriptions, avoiding the The three herbal flavors are prepared separately, plated, developed, developed, and inspected. The operation is simple, the detection cycle is short, and the detection efficiency is high. The thin-layer chromatogram obtained by using this method has good separation, clear color development of spots, and avoids ammonia water, The use of irritating reagents such as chloroform and glacial acetic acid.

Description

technical field [0001] The present disclosure relates to a thin-layer identification method for extracts and preparations of Baoyuan Decoction and similar recipes, belonging to the field of traditional Chinese medicine detection methods. Background technique [0002] Baoyuan Decoction, the formula comes from Baoyuan Decoction in "Catalogue of Ancient Classic Famous Recipes (First Batch)", and the medicinal flavors are ginseng, astragalus, licorice, cinnamon and ginger. Classical prescriptions refer to the prescriptions recorded in ancient Chinese classics that are still widely used, have definite curative effects, and have obvious characteristics and advantages. The state encourages product development based on classic prescriptions. [0003] Baoyuan Decoction is similar to the formula, which refers to the clinical application of doctors of the past dynasties, based on Baoyuan Decoction to adjust the dosage of drugs, or to appropriately increase or decrease the composition o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/90G01N30/94G01N1/34G01N1/38
CPCG01N30/90G01N30/94G01N1/34G01N1/38
Inventor 朱旭伟傅苗青寇强杨政周白水
Owner 浙江金城阜通制药有限公司
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