Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Hydrogel-based trace whole blood separation and plasma detection integrated microfluidic chip

A microfluidic chip and hydrogel technology, applied in the biological field, can solve problems such as limiting the convenience of detection, achieve the effect of improving portability, improving utilization rate, and realizing minimally invasive instant testing

Active Publication Date: 2019-12-03
SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV +1
View PDF10 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the reported blood separation chips are all applied to the separation of milliliter-level blood samples, which need to be combined with professional venous blood collection, and cannot be well combined with minimally invasive blood collection methods such as fingertip blood collection commonly used in community medical care and home health care. The convenience of detection

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Hydrogel-based trace whole blood separation and plasma detection integrated microfluidic chip
  • Hydrogel-based trace whole blood separation and plasma detection integrated microfluidic chip
  • Hydrogel-based trace whole blood separation and plasma detection integrated microfluidic chip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1. Preparation of microfluidic chip for separation of whole blood based on hydrogel and plasma detection

[0042] 1.1 Experimental materials

[0043] 1.1.1 Experimental reagents:

[0044] 1) Polydimethylsiloxane (Polydimethylsiloxane, PDMS) prepolymer and its initiator were purchased from Dow Corning, USA;

[0045]2) Negative photoresist SU-8 2050 and its developer were purchased from Micro Chem in the United States;

[0046] 3) Polycarbonate membrane (Polycarbonate membrane, pore size 1 μm), purchased from MerckMillipore, USA;

[0047] 4) IGF-1 protein detection kit (Human IGF-I DuoSet ELISA kit) was purchased from American R&D Company.

[0048] 5) TMB Chromogenic Kit, purchased from Shanghai Sangon Bioengineering Co., Ltd.

[0049] 6) 3-(Trichlorosilyl)propylmethacrylate (3-(Trichlorosilyl)propylmethacrylate, TMB), purchased from Fluka Chemicals, USA.

[0050] 7) Paraffin oil (Paraffin), purchased from Acros Company in the United States.

[0051] 8) Amin...

Embodiment 2

[0093] Example 2, Characterization of PEGDA hydrogel in microfluidic chip

[0094] Determination of Hydrogel's Ability to Immobilize Microspheres

[0095] experimental method:

[0096] Pipette 10 μL of the prepared microsphere-photocrosslinking prepolymer mixture and drop it on the glass slide.

[0097] Under the fluorescent inverted microscope, use the ultraviolet excitation light of the microscope to expose for 15s, so that the exposed part forms a hydrogel microcolumn, and take pictures for recording.

[0098] Use 50 μL of PBS buffer to wash the samples on the slide three times, observe the results under a microscope and take pictures for recording.

[0099] Experimental results:

[0100] figure 2 It shows that the hydrogel microcolumn can wrap the polystyrene microsphere firmly so that it cannot be washed away by the solution. The experimental results show that the PEGDA hydrogel under the conditions of this study can be used as the immobilization carrier of the micr...

Embodiment 3

[0113] Example 3, Microfluidic chip adsorption protein solution identification

[0114] experimental method:

[0115] The whole blood sample was used as the experimental group, the hemolyzed plasma sample was used as the positive control, and the PBS buffer was used as the negative control, and 2 μL of the sample and the buffer were injected into the microfluidic chip, and allowed to stand for 10 minutes.

[0116] Disassemble the chip, take out the micropad with pointed tweezers, place it on a clean cover glass prepared in advance, and observe it in front of a white background.

[0117] Experimental results:

[0118] Figure 5 Shows the whole process of the hydrogel chip absorbing plasma from whole blood: first inject the immune microsphere-PEGDA hydrogel prepolymer mixture into the lower channel, and form a hydrogel by ultraviolet exposure; then inject whole blood into the upper channel; plasma After infiltrating the filter membrane, it contacts the hydrogel and is adsorbe...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
pore sizeaaaaaaaaaa
Login to View More

Abstract

The invention discloses a hydrogel-based trace whole blood separation and plasma detection integrated microfluidic chip. In the chip, hydrogel is obtained through crosslinking of PEGDA (poly(ethyleneglycol) diacrylate), a photoinitiator and immunomicrospheres. The microfluidic chip comprises an incubation channel located on the upper layer, a plasma separation detection channel located on the lower layer and a filter membrane located between the upper layer and the lower layer; two ends of the incubation channel are open and are a sample inlet and a sample outlet respectively; one end of a whole blood separation detection channel is open; and the whole blood separation detection channel is filled with a PEGDA hydrogel system. Steps of blood testing can be effectively simplified, minimallyinvasive POCT (point-of-care testing) of community health care and home health care is realized, and real-time monitoring of health state of residents and early warning and diagnosis of diseases arefacilitated.

Description

technical field [0001] The invention belongs to the field of biology, and relates to a hydrogel-based integrated microfluidic chip for separating trace whole blood and detecting plasma. Background technique [0002] Point-of-care Testing (POCT) refers to a rapid test and analysis of samples immediately after sampling, which saves the complicated processing procedures and expensive professional equipment of laboratory tests. Clinical, community, home and personalized 1 . The detection technology based on microfluidic chips (Microfluidic Chips) can realize chemical analysis or biological detection in micron-scale flow channels and reaction chambers. It has the advantages of miniaturization, portability, easy operation, less sample consumption, and high detection sensitivity. Meet the requirements of instant inspection to the greatest extent 2 . However, most of the current microfluidic chip detection technologies require additional blood sample pretreatment steps 3,4 . I...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N33/544G01N1/28B01L3/00
CPCG01N33/54306G01N33/54313G01N33/544G01N1/28B01L3/5027B01L2300/0887B01L2300/12
Inventor 张雅鸥李威高丹胡绍良邓凤林谢伟东许乃寒
Owner SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com