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Method for rough purification of living cyanea nozakii protein toxin

A white jellyfish and toxoid technology, applied in the field of marine biology, can solve the problems of non-recyclable continuous use, unfavorable collection of jellyfish toxins, and long time consumption, and achieve the effects of shortening production time, production sustainability advantages, and low possibility

Inactive Publication Date: 2017-10-27
熊廷珍
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method has many disadvantages. For example, the jellyfish tentacles can only be used once and cannot be recycled continuously. Even if the jellyfish tentacles can be regenerated, it will take 7-10 days to complete the regeneration, which takes a long time and is not conducive to the collection of industrial jellyfish toxins. accomplish

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Put 20g of cornstarch, 2g of xanthan gum, and 78g of purified water into a stainless steel pot, heat and boil, stir continuously until there is no precipitation, then pour it into a stainless steel square tank with a length of 20cm, a width of 12cm, and a depth of 2cm to cool and solidify. Cover the nylon gauze with a mesh diameter of 2 mm that has been cleaned. When it is not completely solidified, cover it with a layer of nylon gauze. After solidification, carefully demould. Fix the net-covered starch solidified body on stainless steel or plastic extension sticks, close to natural waters or artificially cultivated live 10 white jellyfish with an umbrella diameter of 25cm, stir slightly, and induce the jellyfish to defend against them. After each one is collected for 10 minutes , recover the solidified starch covering the net, rinse it with purified water, carefully tear off the nylon net, cut off the starch solidified body with a thickness of 5mm on both sides with a s...

Embodiment 2

[0020] Add 15g of potato starch, 4g of carrageenan, and 81g of purified water into a stainless steel pot and heat to boil. Stir continuously until there is no precipitation, then pour it into a stainless steel square tank with a length of 20cm, a width of 12cm, and a depth of 2cm to cool and solidify. The nylon gauze with a mesh diameter of 2mm has been washed. Before it is completely solidified, cover it with a layer of nylon gauze. After solidification, carefully remove the mold. Fix the net-covered starch solidified body on stainless steel or plastic extension sticks, close to natural waters or artificially cultivated live 10 white jellyfish with an umbrella diameter of 25cm, stir slightly, and induce the jellyfish to defend against them. After each one is collected for 10 minutes , recover the solidified starch covered with net, rinse it with purified water, carefully tear off the nylon net, cut off the starch solidified with a thickness of 5mm on both sides with a stainles...

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PUM

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Abstract

The present invention proposes a crude purification method of living white jellyfish protein toxoid, which adopts the method of starch coagulation preparation, coating, toxin collection, enzymolysis, centrifugation, nickel column chromatography, and ultrafiltration to remove impurities to obtain white jellyfish Protein toxoid crude pure solution. Compared with the prior art, the beneficial effect of the present invention is that only the nematocysts of the white jellyfish and their toxic substances are collected, the damage to the jellyfish is small, the regeneration time is short, only 2 days, and the production is extremely sustainable Advantages provide conditions for the future industrial use of jellyfish toxins; using enzymatic methods to decompose starch solidification time is shorter than jellyfish autolysis time, which is beneficial to shorten production time; collected nematocysts and their toxins are less likely to be polluted by external proteins, The protein isolated by preliminary purification is only aequorin toxin protein, which creates a good foundation for subsequent further purification.

Description

technical field [0001] The invention belongs to the technical field of marine organisms, and in particular relates to a method for crudely purifying protein toxoids from living white jellyfish. Background technique [0002] Jellyfish is a common marine zooplankton belonging to the phylum Cnidari. There are many species. There are about a thousand species known in the world. They are widely distributed in temperate, subtropical and tropical seas. They are marine omnivores. Common species in sea areas include moon jellyfish, white jellyfish, jellyfish and jellyfish. The tentacles of jellyfish are densely covered with nematocysts, which contain a large number of toxins, which play a very important role in jellyfish defense and predation. The toxin composition and toxicity of different types of jellyfish vary greatly. Studies have shown that jellyfish toxins are not single Substances, which contain proteins of various toxic components, mainly including cardiovascular toxicity, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/435C07K1/14
CPCC07K14/43595
Inventor 潘爱芹
Owner 熊廷珍
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