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A flavoring compound microbial preparation for rapid fermentation of tobacco leaves and its application

A technology of compounding microorganisms and tobacco leaves, applied in the field of cigarette microorganisms, can solve the problems of insignificant aroma enhancement effect, complicated technical application, narrow application range, etc., and achieve the effects of shortening the alcoholization time, simple use and quality improvement.

Active Publication Date: 2020-11-06
CHINA TOBACCO SHAANXI IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the current studies use a single strain of fermentation or microbial preparations can only target a single type of tobacco leaves (such as flue-cured tobacco), which has a narrow scope of application, and there are also problems such as ineffective aroma enhancement, long fermentation time, complicated technical applications, and difficult cultivation. And other issues

Method used

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  • A flavoring compound microbial preparation for rapid fermentation of tobacco leaves and its application
  • A flavoring compound microbial preparation for rapid fermentation of tobacco leaves and its application
  • A flavoring compound microbial preparation for rapid fermentation of tobacco leaves and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Bacillus ( Bacillus subtilis ) Y1, Bacillus ( Bacillus subtilis )X7 and Bacillus ( Bacillus subtilis ) Separation and screening of X8:

[0026] 1. Isolate tobacco aroma-imparting bacteria strains from the surface of naturally aged tobacco leaves, the steps are: first mix 90mL sterile water with 10g aged tobacco leaves (i.e. 10 -1 Suspension), use aseptic sampling method to take 1mL of the mixture and add it to 9mL sterile water to make 10 -2 suspension, and then serially diluted, take 10 -2 、10 -3 、10 -4 、10 -5 、10 -6 、10 -7 Spread 0.2mL of suspensions of six dilutions on the improved LB medium, incubate at a constant temperature of 37°C for 24 hours, pick a single colony on a plate with good growth, full and uniform colonies, and appropriate dilution, and examine under the microscope.

[0027] If the microscopic examination shows a purely cultured single strain, inoculate it on a modified LB slant solid medium, incubate at a constant temperature of 37°C for 24...

Embodiment 2

[0037] The bacillus ( Bacillus subtilis ) Y1, Bacillus ( Bacillus subtilis ) X7, Bacillus ( Bacillus subtilis )X8 is compounded according to the mass ratio of 1:1:1 to obtain a fragrance-imparting microbial preparation, which is called preparation 1;

[0038] The bacillus ( Bacillus subtilis ) Y1, Bacillus ( Bacillus subtilis ) X7, Bacillus ( Bacillus subtilis )X8 is compounded according to the mass ratio of 0.001:1:0.001 to obtain a fragrance-imparting microbial preparation, which is called preparation 2;

[0039] The bacillus ( Bacillus subtilis ) Y1, Bacillus ( Bacillus subtilis ) X7, Bacillus ( Bacillus subtilis )X8 is compounded according to the mass ratio of 1:0.001:1 to obtain a fragrance-imparting microbial preparation, which is called preparation 3;

[0040] The bacillus ( Bacillus subtilis ) Y1, Bacillus ( Bacillus subtilis ) X7, Bacillus ( Bacillus subtilis )X8 is compounded according to the mass ratio of 0.005:0.003:0.008 to obtain a fragranc...

Embodiment 3

[0044] The aroma-imparting compound microbial preparation prepared in Example 2 is used for the rapid fermentation of tobacco leaf raw materials, and the tobacco leaf raw materials are sliced, loosened and rehydrated, and then shredded to obtain raw tobacco shredded tobacco, at a rate of 0.0001wt% to 0.5wt% % Weigh the aroma-imparting compound microbial preparation prepared in Example 2, spray it on the surface of the shredded tobacco, and ferment at a temperature of 25°C to 45°C and a relative humidity of 30% to 90% for 24h to 60h at constant temperature and humidity. After drying the cut tobacco, the water content is between 12% and 14%, and the raw cut tobacco of tobacco leaves fermented with the aroma-imparting compound microorganism preparation is obtained.

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Abstract

The present invention discloses a fragrant composite microbial preparation for rapid fermentation of tobacco leaves. The fragrant composite microbial preparation comprises bacillus subtilis Y1, bacillus subtilis X7 and bacillus subtilis X8 in a mass ratio of (0.001-1) :(0.001-1) :(0.001-1), wherein the three kinds of bacillus subtilis are preserved in CGMCC (China General Microbiological Culture Collection Center) with preservation numbers of CGMCC No. 13602, CGMCC No. 13603 and CGMCC No. 13604. The invention further discloses an application of the composite microbial preparation in fermentation of tobacco leaf raw materials. The tobacco leaf raw materials are sliced, subjected to resurgence treatment and shredded; the fragrant composite microbial preparation is sprayed to the surface of shredded tobacco, aAnd the tobacco is fermented at constant temperature and humidity and dried. The fragrant composite microbial preparation can significantly improve the quality and sensory quality of single-grade raw tobacco leaves and leaf combinations.

Description

technical field [0001] The invention belongs to the technical field of cigarette microorganisms, and relates to an aroma-imparting composite microbial preparation for rapid fermentation of tobacco leaves and an application thereof. Background technique [0002] Alcoholization of tobacco leaves is an important link in the tobacco processing process, which has an important impact on the formation of smoking quality of tobacco leaves. This process is accompanied by complex physical, physiological, biochemical and chemical reactions. The macromolecular organic substances and chemical components in tobacco leaves are continuously decomposed, transformed and consumed under the action of oxidation and enzymatic reactions, and small molecular organic substances are continuously formed, which promotes the development of tobacco leaves. Changes in the aroma quality of tobacco leaves. [0003] Generally, the fermentation method of tobacco leaves in tobacco industry enterprises adopts ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A24B3/12C12R1/125
CPCA24B3/12C12N1/20
Inventor 薛磊郭志刚王颖牟含军郭斌黄仲波胡喜怀
Owner CHINA TOBACCO SHAANXI IND
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