Maize molecule assisted breeding method

A corn breeding and corn technology, applied in the biological field, can solve the problems of slow progress of QTL research on corn salt tolerance and other problems

Inactive Publication Date: 2017-11-17
CHINA AGRI UNIV
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Problems solved by technology

However, research on QTLs for salt tolerance in maize has been slow

Method used

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  • Maize molecule assisted breeding method
  • Maize molecule assisted breeding method
  • Maize molecule assisted breeding method

Examples

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Embodiment 1

[0070] Example 1. Maize molecular markers are related to maize yield-related traits, flowering time traits and stress resistance traits

[0071] This embodiment provides a corn molecular marker and a corn breeding method. The corn molecular marker is marked as ZaMM, and ZaMM is the DNA fragment shown in the 89th-5210th position of sequence 1 in the sequence listing.

[0072] 1. Maize molecular markers

[0073] There are three cases of ZaMM in maize. The first is that both chromosomes of the maize genome contain the DNA fragment shown in the 89-5210 position of sequence 1, and the second is that the two chromosomes of the maize genome do not contain sequence 1. The third is the DNA fragment shown in the 89-5210 of the sequence 1, the third is the two chromosomes of the maize genome, one contains the DNA fragment shown in the 89-5210 of the sequence 1, and the other does not contain the 89-5210 of the sequence 1 For the DNA fragment shown, the genotype of maize whose chromosome...

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Abstract

The invention discloses a maize molecule assisted breeding method. The maize molecule assisted breeding method comprises the steps as follows: hybridizing maize A of genotype AA or genotype AB and maize B of genotype BB to obtain first-filial generation F1; backcrossing the first-filial generation F1 and maize B 3 to 8 times, selecting the maize of genotype AB for backcrossing with the maize B each time to obtain backcross descendants, selecting the maize of the genotype AB from the backcross descendants for selfing to obtain self-bred progenies, and selecting the maize of the genotype AA from the self-bred progenies to obtain a target maize self-bred line. The maize of the genotype AB of the backcross descendants has characteristics of long tasseling period, long pollinating period and/or long spinning period and characteristics of disease resistance and stress resistance. Experiments prove that with adoption of the maize breeding method, the maize self-bred line with disease resistance, stress resistance and excellent yield trait potential can be obtained.

Description

technical field [0001] The invention relates to a method for corn molecular assisted breeding in the field of biotechnology. Background technique [0002] Reproduction-related genes directly determine various reproductive-related traits of organisms, and also determine the adaptability of organisms to the environment in terms of reproductive methods. The flowering time of higher plants is an important trait related to reproduction, which directly limits the geographical conditions such as latitude where plants can survive. In addition, flowering period is also a trait closely related to grain crop yield. [0003] There are mainly (1) photoperiod pathway, (2) hormone pathway, and (3) autonomous pathway to regulate the flowering period of maize. The traits of flowering stage in maize mainly include tasseling stage, pollination stage and silking stage, which belong to quantitative genetic traits. There are many genes that affect the flowering traits of maize, and it is diffi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68A01H1/02
CPCA01H1/02C12Q1/6895C12Q2600/13
Inventor 徐明良李懿璞童丽秀邓乐乐杨小红刘保申邢跃先
Owner CHINA AGRI UNIV
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