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Method for extracting and measuring aloe polysaccharide from aloe

A technology of aloe polysaccharides and determination methods, which is applied in the preparation of test samples, color/spectral characteristic measurement, etc., can solve the problems of little increase in extraction rate and low purity of aloe polysaccharides, and achieve short processing time and high product yield , the effect of simple operation steps

Inactive Publication Date: 2017-11-24
BAISE UNIV
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

However, the purity of the extracted aloe polysaccharide is not high, and the extraction rate is not improved much.

Method used

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  • Method for extracting and measuring aloe polysaccharide from aloe
  • Method for extracting and measuring aloe polysaccharide from aloe

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Experimental program
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Effect test

Embodiment 1

[0030] A method for extracting and measuring aloe polysaccharides in aloe, selecting Aloe arborescens as the research object, comprising the following steps,

[0031] S1. Prepare samples: Pick fresh and tender aloe vera leaves, clean the dust on the aloe vera leaves with clean water, then gently remove the thorns on the leaves with a scalpel, and then cut them into 5mm slices and spread them on the tray Then put it in an oven, bake it at 55°C for 12 hours, and finally crush the dried aloe leaves into powder to make aloe powder, the aloe powder is 60 mesh, put it in a brown reagent bottle to avoid light Label and set aside.

[0032] S2, microwave treatment: take the aloe powder prepared in step S1, add 20ml of distilled water to obtain an extract, and process the extract with microwaves, the power of the microwave treatment is set to 700W, and the microwave time is controlled so that the temperature of the pretreatment solution reaches 80 until ℃.

[0033] S3. Ultrasonic extr...

Embodiment 2

[0048] A method for extracting and measuring aloe polysaccharides in aloe, selecting Aloe arborescens as the research object, comprising the following steps,

[0049] S1. Prepare samples: pick fresh and tender aloe vera leaves, clean the dust on the aloe vera leaves with clean water, then gently remove the thorns on the leaves with a scalpel, and then cut them into 4 mm thin slices and spread them on the tray and then put it in an oven, and dry it at 50°C for 11 hours, and finally crush the dried aloe leaves into powder to make aloe powder, the aloe powder is 40 mesh, put it in a brown reagent bottle to avoid light Label and set aside.

[0050] S2. Microwave treatment: take the aloe powder prepared in step S1, add 20ml of distilled water to obtain an extract, and process the extract with microwaves. The power of the microwave treatment is set to 600W, and the microwave time is controlled so that the temperature of the pretreatment liquid reaches up to 75°C.

[0051] S3. Ultr...

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Abstract

The invention provides a method for extracting and measuring aloe polysaccharide from aloe. The extraction method comprises the steps of preparing samples, performing microwave treatment, performing ultrasonic extraction, performing ultrasonic extraction for the second time and measuring the aloe polysaccharide. The extraction adopts the synergistic effect of a microwave assistance method and an ultrasonic assistance method, and has the advantages of simple operating steps, capability of saving solvents, high product yield, short treatment time and the like. The aloe polysaccharide content is measured by a spectrophotometry, so the method is simple and rapid, small in result error and high in stability.

Description

technical field [0001] The invention relates to the technical field of biopharmaceuticals, in particular to an extraction method of aloe polysaccharides in aloe. Background technique [0002] Aloe extract is an important raw material in medicine, chemical industry and food, and aloe polysaccharide is one of the main functional components in aloe extract. Aloe polysaccharides can improve the immune function and anti-radiation effect of the human body, and have certain pharmacological therapeutic effects on anti-cancer and anti-AIDS virus. [0003] However, the content of aloe polysaccharides is low, and it is very difficult to separate, extract and purify. The traditional method of extracting aloe polysaccharides is hot water extraction, which has low yield, time-consuming operation and high energy consumption. Ultrasound-assisted method is a new type of auxiliary extraction technology, which has the advantages of simple operation steps, solvent saving, and short processing...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00G01N21/31G01N1/28
CPCC08B37/0003G01N1/28G01N21/31
Inventor 谢晓娜杨郑州张真真
Owner BAISE UNIV
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