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Preparation method of standardized in vitro tumor micro-tissues

A tumor tissue and micro-tissue technology, which is applied in the preparation, sampling, and measurement devices of test samples, can solve problems such as difficulty in processing, affect data reliability, and errors, and achieve the effect of mutual comparison.

Inactive Publication Date: 2017-12-01
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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Problems solved by technology

However, the properties and texture of tumor tissue determine that it is not easy to handle in vitro, and the operation is completely judged by the naked eye, which has a large randomness, which brings large errors to the follow-up research and affects the reliability of the obtained data.
This restricts the development of tumor tissue in vitro studies

Method used

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  • Preparation method of standardized in vitro tumor micro-tissues
  • Preparation method of standardized in vitro tumor micro-tissues
  • Preparation method of standardized in vitro tumor micro-tissues

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Embodiment 1

[0028] The tumor tissue isolated from patients with solid tumors (breast cancer) after surgery is processed in a sterile environment in the shortest possible time. Tumor samples from non-necrotic parts were excised, immediately immersed in 4°C pre-cooled sterile vials filled with PBS, and delivered to the laboratory within 3 hours. The PBS contains 100U / mL penicillin and 100U / mL streptomycin; in a biological safety cabinet, transfer the tumor sample to a 100mm cell culture dish, rinse with 1×PBS 10mL three times, wash away the blood cells, and peel off the surface of the tumor sample Connective tissue, fibers and fat, the treated tumor tissue was transferred to a new 100mm Petri dish, 0.5mL PBS was added dropwise, and the tumor tissue was divided into diameters less than 1mm with a sterile scalpel blade, surgical scissors and surgical forceps 3 fragments; the minced tumor tissue was transferred to a 50mL centrifuge tube, and centrifuged at 500 rpm for 2 minutes in a desktop ce...

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Abstract

The invention provides a preparation method of standardized in vitro tumor micro-tissues, belonging to the technical field of biological medicines. The preparation method comprises the steps of dividing a solid tumor tissue into small blocks, and carrying out interception by virtue of a porous sieve according to the diameter size of the tumors, so as to obtain uniform-size tumor micro-tissues. The preparation method can be applied to the culture and drug evaluation of tumor tissues. The tumor tissues prepared by virtue of the preparation method are controllable in sizes and convenient to use and can be standardized for subsequent operation. According to the preparation method, the problem that the in vitro culture of the tumor tissues is difficult to be standardized is solved; and meanwhile, the used tumor tissues are not subjected to in vitro digestion and dispersion, so that the components and characters of the tumor tissues are preserved, and the effects of the tumor tissues are guaranteed.

Description

technical field [0001] The invention relates to the field of in vitro tumor tissue culture, in particular to a method for preparing tumor microtissues. Background technique [0002] Cancer is a malignant disease that seriously threatens human health, and has become one of the main causes of death affecting the health of Chinese residents. Experts from the World Health Organization (WHO) predict that the global population will be 8 billion in 2020, new cancer cases will reach 20 million, and 12 million people will die of cancer. Cancer will become the first killer of human beings in the new century and become the largest public health problem in the world one of the problems. Therefore, it is extremely important to take effective measures to treat cancer. [0003] The prognosis of many types of cancer remains very poor, and there is an urgent need to develop new treatment options, which requires in-depth study of tumors. However, how to effectively carry out tumor research...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N1/34
CPCG01N1/28G01N1/286G01N1/30G01N1/34G01N2001/2873
Inventor 秦建华李中玉
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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