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Rifamycin extracting and purifying method

A technology of rifamycin and purification method, which is applied in the direction of organic chemistry to achieve the effects of avoiding pollution problems, reducing environmental protection treatment costs, and low production costs

Active Publication Date: 2017-12-15
HEILONGJIANG HUARUI BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to overcome the defects of the above-mentioned prior art, to provide a method that effectively improves the extraction yield, increases the effective content of the product, improves the product quality, and has a simple process and low cost, effectively solves the problem of environmental pollution, and reduces the cost of environmental treatment. Extraction and purification method of rifamycin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Rifamycin fermentation broth 50m 3 , The fermentation unit is 20470u / ml, and the total fermentation is 1023.5 billion.

[0041] 1) Pretreatment of fermentation broth

[0042] Add water to the rifamycin fermentation broth to control the protein content in the fermentation broth to 2.5%, then add 150 kg of ammonium sulfate, stir for 40 minutes, and then stand for 60 minutes.

[0043] Adjust the pH of the rifamycin fermentation broth to 9.5 with liquid caustic soda, stir for 40 minutes, and then stand for 110 minutes.

[0044] The inorganic ceramic membrane filtration system is used to process the rifamycin fermentation broth: the temperature of the fermentation broth is raised to 41°C, and the pressure difference is controlled at 0.08~0.1MPa. The first filtration: the pore size of the electrodeless ceramic membrane is controlled at 30um; the second filtration: the pore size of the electrodeless ceramic membrane is controlled at 10um, after solid-liquid separation, 76m of rifamyci...

Embodiment 2

[0061] Rifamycin fermentation broth 50m 3 , The fermentation unit is 20831u / ml, and the total fermentation is 1041.55 billion.

[0062] 1) Pretreatment of fermentation broth

[0063] Add drinking water to the rifamycin fermentation broth to control the protein content in the fermentation broth to 2.6%, then add 175 kg of ammonium sulfate, stir for 45 minutes, and then stand for 65 minutes.

[0064] Adjust the pH of the rifamycin fermentation broth to 9.6 with liquid caustic soda, stir for 45 minutes, and then stand for 120 minutes.

[0065] The inorganic ceramic membrane filtration system is used to process the rifamycin fermentation broth. The temperature of the fermentation broth is raised to 42°C, and the pressure difference is controlled at 0.08~0.1MPa. The first filtration: the pore size of the electrodeless ceramic membrane is controlled at 30um; the second filtration: the pore size of the electrodeless ceramic membrane is controlled at 10um, after solid-liquid separation, 74m ...

Embodiment 3

[0082] Rifamycin fermentation broth 50m 3 , The fermentation unit is 21006u / ml, and the total fermentation is 1050.3 billion.

[0083] 1) Pretreatment of fermentation broth

[0084] Add drinking water to the rifamycin fermentation broth to control the protein content in the fermentation broth to 2.7%, then add 200 kg of ammonium sulfate, stir for 50 minutes, and then stand for 70 minutes.

[0085] Adjust the pH of the rifamycin fermentation broth to 9.7 with liquid caustic soda, stir for 50 min, and then stand for 130 min.

[0086] The inorganic ceramic membrane filtration system is used to process the rifamycin fermentation broth. The temperature of the fermentation broth is raised to 43°C, and the pressure difference is controlled at 0.08~0.1MPa. The first filtration: the pore size of the electrodeless ceramic membrane is controlled at 30um; the second filtration: the pore size of the electrodeless ceramic membrane is controlled at 10um, after solid-liquid separation, 75m of rifamy...

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Abstract

The invention relates to a rifamycin extracting and purifying method. The method includes the steps that water is added into rifamycin fermentation liquor to dilute the rifamycin fermentation liquor, then ammonium sulfate is added, the mixed solution is standing still after being stirred fully, the pH value of the solution is adjusted to be 9.5-10.0, the mixed solution continues to be stirred, is standing still secondarily, and then treated through an inorganic ceramic membrane filtering system, and the rifamycin solution is obtained after solid-liquid separation; the pH value of the obtained rifamycin solution is adjusted to be 7.0-7.5, then the rifamycin solution is treated through an expanded bed, crystallized and dried to obtain the pure rifamycin product. By means of the method, rifamycin impurities are effectively separated, the extraction yield is increased, finished product quality is improved, and the method is simple in process and low in cost, effectively solves the environment pollution problem, reduces environment treatment cost and is beneficial for enhancing product competitiveness in domestic and foreign markets.

Description

Technical field [0001] The invention belongs to the technical field of antibiotic extraction, and particularly relates to a rifamycin extraction and purification method. Background technique [0002] Rifamycin is a class of Ansa macrolide antibiotics produced by Amycobacterium mediterraneanum. This antibiotic has low toxicity, high efficacy and broad antibacterial spectrum, and is widely used in the treatment of G + (Gram-positive bacteria), tuberculosis, leprosy and AIDS-related mycobacterial infections. Since the discovery of rifamycin in the late 1950s, a large number of studies have been carried out on its structure, properties and fermentation technology, and the fermentation unit has been greatly improved. However, in recent years, the industrialization level of rifamycin extraction and purification in my country has increased Slow, the problems are: [0003] 1 The extraction and purification yield is low, and the total extraction yield is 60-65%. [0004] 2 The effective con...

Claims

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Application Information

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IPC IPC(8): C07D498/08
CPCC07D498/08
Inventor 赵海兵王大伟
Owner HEILONGJIANG HUARUI BIOTECHNOLOGY CO LTD