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Vaccine composition preventing porcine reproductive and respiratory syndrome, preparation method and application thereof

A technology for respiratory syndrome and vaccine composition, which is applied in the field of vaccine composition and its preparation for the prevention of porcine reproductive and respiratory syndrome, can solve the problems of gene sequence changes, complex disease, and unsatisfactory re-prevention effect, and achieve improved Productivity, effects of good immunogenicity

Pending Publication Date: 2018-01-05
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, with the application of porcine reproductive and respiratory syndrome live vaccines, the outbreak of porcine reproductive and respiratory syndrome has been controlled to a certain extent, but some new problems have also emerged, especially in the past year, multi-regional epidemiological surveys It was found that in pig farms immunized with porcine reproductive and respiratory syndrome live vaccine, there was a new PRRSV epidemic, and its gene sequence was greatly changed compared with the previous epidemic strains
However, the re-prevention effect of existing vaccines against this situation is not very satisfactory clinically, and the mixed infection of new PRRSV epidemic strains with highly pathogenic PRRSV and classic PRRSV causes the complexity of the clinical condition. Therefore, it is necessary to Developed a vaccine composition targeting the latest clinical situation in my country to effectively prevent the epidemic of new porcine reproductive and respiratory syndrome in China

Method used

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  • Vaccine composition preventing porcine reproductive and respiratory syndrome, preparation method and application thereof
  • Vaccine composition preventing porcine reproductive and respiratory syndrome, preparation method and application thereof
  • Vaccine composition preventing porcine reproductive and respiratory syndrome, preparation method and application thereof

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Experimental program
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Embodiment approach

[0019] As an embodiment of the present invention, the porcine reproductive and respiratory syndrome virus VR2332 strain antigen includes inactivated antigen, attenuated antigen, subunit antigen or synthetic peptide of porcine reproductive and respiratory syndrome virus VR2332 strain or its culture antigen.

[0020] As an embodiment of the present invention, the porcine reproductive and respiratory syndrome virus JXA1 strain antigen includes inactivated antigen, attenuated antigen, subunit antigen or synthetic peptide of porcine reproductive and respiratory syndrome virus JXA1 strain or its culture antigen.

[0021] As an embodiment of the present invention, the porcine reproductive and respiratory syndrome virus HNjz15 strain antigen includes inactivated antigen, attenuated antigen, subunit antigen or synthetic peptide of porcine reproductive and respiratory syndrome virus HNjz15 strain or its culture antigen.

[0022] As an embodiment of the present invention, the antigen o...

Embodiment 1

[0078] Preparation of Antigen of Porcine Reproductive and Respiratory Syndrome Virus JXA1 Strain

[0079] Inoculate the culture of porcine reproductive and respiratory syndrome virus JXA1 strain into Marc-145 cells, insert into the Marc-145 cell culture that forms a single layer according to 1% (V / V) of the virus culture solution, and culture at 37°C , 24-48 hours to harvest the toxic cell culture medium, centrifuge the cells into agglomerates, and discard the supernatant. The cell pellet was resuspended in 0.05M Tris(carboxymethyl)aminomethane 0.025MEDTA buffer containing 0.5% Triton X-100, and the volume of the buffer was 5-10 times that of the concentrated cells. The mixture was stirred at 4°C for 2-15 hours and centrifuged at 10000 g for 1 hour. Harvest the supernatant for later use.

Embodiment 2

[0081] Routine Preparation of Antigen of Porcine Reproductive and Respiratory Syndrome Virus JXA1 Strain

[0082] Inoculate the culture of porcine reproductive and respiratory syndrome virus JXA1 strain into Marc-145 cells, insert into the Marc-145 cell culture that forms a single layer according to 1% (V / V) of the virus culture solution, and culture at 37°C , when the lesion reaches 80%, harvest the toxic cell culture medium, after 2 times of freezing and thawing, collect the poison, and measure the poison price. Add 10% (v / v) formaldehyde solution to make the final concentration of formaldehyde to 0.2% (V / V), inactivate at 37°C for 18 hours, stir once every 4 hours, stir for 10min each time, and set aside after complete inactivation.

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Abstract

The invention relates to a vaccine composition preventing porcine reproductive and respiratory syndrome. Specifically, the vaccine composition includes an immune dosage of classic porcine reproductiveand respiratory syndrome virus antigen and / or highly pathogenic porcine reproductive and respiratory syndrome virus antigen and / or a porcine reproductive and respiratory syndrome virus NADC30 strainantigen, and a pharmaceutically acceptable carrier. The vaccine composition provided by the invention can effectively resist the attack of new epidemic strains, highly pathogenic porcine reproductiveand respiratory syndrome viruses and classic porcine reproductive and respiratory syndrome viruses, solves the infection problem of porcine reproductive and respiratory syndrome viruses of different sources of the, and is of important significance for purification of porcine reproductive and respiratory syndrome viruses.

Description

technical field [0001] The invention belongs to the field of veterinary vaccines, and in particular relates to a vaccine composition for preventing porcine reproductive and respiratory syndrome, a preparation method and application thereof. Background technique [0002] Porcine reproductive and respiratory syndrome (PRRS) is an infectious disease caused by porcine reproductive and respiratory syndrome virus (PRRSV), characterized by reproductive failure in pregnant sows and respiratory symptoms in piglets. The disease was first discovered in North Carolina in the United States (1987), and then successively found in Canada (1998), Germany (1990), and the United Kingdom (1991). In 1992, the International Veterinary Office (OIE) named the disease Porcine Reproductive and Respiratory Syndrome (PRRS). [0003] Porcine reproductive and respiratory syndrome virus is a single-stranded positive-sense RNA virus with a high mutation frequency due to point mutations, deletions, inserti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/215A61P31/14
CPCA61K39/12C12N7/00A61P31/00
Inventor 张许科孙进忠田克恭张海洋
Owner PU LIKE BIO ENG
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