A diphenyl ether compound with antibacterial activity in honeysuckle and its preparation method and application

A technology of diphenyl ethers and antibacterial activity, applied in the field of natural product chemistry

Active Publication Date: 2020-06-16
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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This compound has not been reported so far

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  • A diphenyl ether compound with antibacterial activity in honeysuckle and its preparation method and application
  • A diphenyl ether compound with antibacterial activity in honeysuckle and its preparation method and application
  • A diphenyl ether compound with antibacterial activity in honeysuckle and its preparation method and application

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Experimental program
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Effect test

Embodiment 1

[0046] The honeysuckle sample comes from Kunming, Yunnan, and the variety tree-shaped honeysuckle No.3.

[0047] A method for preparing diphenyl ether compounds with antibacterial activity in honeysuckle, including the following steps:

[0048] Step (1), extract extraction: use dried honeysuckle as raw material, crush honeysuckle to 30-50 mesh, use 80% methanol aqueous solution as the extraction solvent, soak and extract 3 times, each time soaking for 24h, The weight ratio of extraction solvent to honeysuckle used for the second time is 3:1, the extracts are combined, filtered, and the filtrate is concentrated into an extract;

[0049] Step (2), silica gel column chromatography: first dissolve the extract with methanol 1.5 times the weight of the extract, then mix the sample with 80-100 mesh silica gel 0.8 times the weight of the extract, and then load the sample for column chromatography. , The column silica gel is 160 mesh, the silica gel column method is dry packing, the weight o...

Embodiment 2

[0054] The honeysuckle sample comes from Kunming, Yunnan, and the variety tree-shaped honeysuckle No.3.

[0055] A method for preparing diphenyl ether compounds with antibacterial activity in honeysuckle, including the following steps:

[0056] Step (1), extract extract: use dried honeysuckle as raw material, crush honeysuckle to 30-50 mesh, use methanol as extraction solvent, soak and extract 5 times, soak for 72h each time, and extract the solvent and the weight of honeysuckle each time The ratio is 5:1, the extracts are combined, filtered, and the filtrate is concentrated into an extract;

[0057] Step (2), silica gel column chromatography: the extract is first dissolved with acetone 3 times the weight of the extract, and then mixed with 100 mesh silica gel 1.2 times the weight of the extract, and then loaded for column chromatography. The column silica gel is 300 mesh, and the method of silica gel packing is dry packing. The weight of silica gel used is 3.5 times the weight of t...

Embodiment 3

[0062] The honeysuckle sample comes from Kunming, Yunnan, and the variety tree-shaped honeysuckle No.3.

[0063] A method for preparing diphenyl ether compounds with antibacterial activity in honeysuckle, including the following steps:

[0064] Step (1), extract extraction: use dried honeysuckle as a raw material, crush the honeysuckle to 30-50 mesh, use an ethanol aqueous solution with a weight percentage of 80% as the extraction solvent, soak and extract 4 times, each soaking for 36h, The weight ratio of extraction solvent to honeysuckle used each time is 4:1, the extracts are combined, filtered, and the filtrate is concentrated into an extract;

[0065] Step (2), silica gel column chromatography: first dissolve the extract with acetone twice the weight of the extract, then mix the sample with 90 mesh silica gel twice the weight of the extract, and then load the sample for column chromatography. The column silica gel is 200 mesh, and the method of silica gel packing is dry packing...

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Abstract

The invention relates to a diphenyl ether compound with antibacterial activity in honeysuckle and a preparation method and application thereof, and belongs to the technical field of natural product chemistry. The structural formula of the compound is shown as the formula (I) in the description. The compound is obtained through the steps of extract extraction, silica-gel column chromatography and high-pressure liquid chromatography separation and purification with the traditional medicinal and edible plant honeysuckle as the raw material. An activity test shows that the compound has good bacteriostasis; by adding the compound into tobacco feed liquid, the growth of microorganisms in the feed liquid can be effectively inhibited, and the shelf life of the tobacco feed liquid can be prolonged.Thus, the compound has good application prospects.

Description

Technical field [0001] The invention belongs to the technical field of natural product chemistry, and specifically relates to a diphenyl ether compound extracted for the first time from the traditional medicinal and food homologous plant honeysuckle. At the same time, the present invention also relates to a preparation method of the compound and its application in preventing spoilage and deterioration of tobacco material liquid and extending the shelf life of tobacco material liquid. Background technique [0002] Honeysuckle, also known as Lonicera japonicus, is a perennial semi-evergreen woody, medicinal and edible plant of the Lonicera family. Since it is white at the beginning, it turns to yellow, hence the name honeysuckle. Honeysuckle is a kind of honeysuckle that has been known as a good medicine for clearing away heat and detoxification since ancient times. It has a sweet and fragrant nature, clears away heat and does not hurt the stomach, and is very aromatic and can eli...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C49/84C07C45/78A01N35/04A01P3/00A01P1/00A24B15/34
Inventor 张承明孔维松刘欣李晶耿永勤李雪梅米其利刘志华王明峰者为周敏杨光宇胡秋芬李干鹏
Owner CHINA TOBACCO YUNNAN IND
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