Flavonoid compound extracted from aloes as well as preparation method and application thereof

A technology of flavonoids and compounds, applied in the field of phytochemistry, to achieve the effects of prolonging the shelf life, simple preparation process, and wide source of raw materials

Active Publication Date: 2018-02-23
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The compound and its application in inhibiting the spoilage of tobacco material liquid and prolonging the shelf life of tobacco material liquid have not been reported yet.

Method used

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  • Flavonoid compound extracted from aloes as well as preparation method and application thereof
  • Flavonoid compound extracted from aloes as well as preparation method and application thereof
  • Flavonoid compound extracted from aloes as well as preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0044] The preparation of embodiment 1-compound

[0045] The aloe samples were from Kunming, Yunnan, and the species was Aloe vera. Sampling 2.0 kg of dried aloe vera was crushed to 50 mesh, extracted 5 times with 95% methanol for 24 hours each time, combined the extracts, filtered, and concentrated under reduced pressure to obtain 115 g of extract. The extract is dissolved in pure methanol with a weight ratio of 2.0 times, and then mixed with 130 g of 100-mesh silica gel, and 0.8 kg of 160-mesh silica gel is packed into a column for silica gel column chromatography. The volume ratio is 1:0, 20:1, 9:1, 8:2, 7:3, 6:4, 1:1, 1:2 chloroform-acetone gradient elution, TLC monitoring and merging the same parts to obtain 8 parts, wherein the volume ratio is 7: The chloroform-acetone eluted part of 3 was separated by Agilent 1100 semi-preparative high-performance liquid chromatography, with 52% methanol as mobile phase, Zorbax SB-C18 (21.2×250mm, 5 μm) preparative column as stationary...

Embodiment 2

[0046] The preparation of embodiment 2-compound

[0047] The aloe sample comes from Yuanjiang, Yunnan, and the variety is Chinese aloe. The dried aloe sample is crushed to 30 meshes, 3.6 kg is sampled, extracted 4 times with 95% ethanol, and extracted for 48 hours each time. The extracts are combined, filtered, and concentrated under reduced pressure to obtain Extract 265g. The extract is dissolved in pure methanol with a weight ratio of 2.0 times, and then mixed with 280g of 80 mesh thick silica gel, and 1.4kg of 200 mesh silica gel is packed for silica gel column chromatography, and the volume ratio is 1:0, 20:1, 9:1, 8:2, 7:3, 6:4, 1:1, 1:2 chloroform-acetone gradient elution, TLC monitoring and merging the same parts to obtain 8 parts, wherein the volume ratio is 7: The chloroform-acetone eluted part of 3 was separated by Agilent 1100 semi-preparative high-performance liquid chromatography, with 52% methanol as mobile phase, Zorbax SB-C18 (21.2×250mm, 5 μm) preparative co...

Embodiment 3

[0048] The preparation of embodiment 3-compound

[0049] The aloe sample came from Sanming, Fujian. 5.5 kg of the aloe sample was crushed to 40 meshes, extracted three times with 75% acetone by ultrasound, each time for 72 hours, the extracts were combined, filtered, and concentrated under reduced pressure to obtain 396 g of extract. The extract is dissolved in pure methanol with a weight ratio of 1.6 times, and then mixed with 450 g of 90 mesh thick silica gel, and 2.8 kg of 180 mesh silica gel is packed for silica gel column chromatography, and the volume ratio is 1:0, 20:1, 9:1, 8:2, 7:3, 6:4, 1:1, 1:2 chloroform-acetone gradient elution, TLC monitoring and merging the same parts to obtain 8 parts, wherein the volume ratio is 7: The chloroform-acetone eluted part of 3 was separated by Agilent 1100 semi-preparative high-performance liquid chromatography, with 52% methanol as mobile phase, Zorbax SB-C18 (21.2×250mm, 5 μm) preparative column as stationary phase, and the flow r...

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Abstract

The invention discloses a flavonoid compound extracted from aloes as well as a preparation method and application thereof. The flavonoid compound has a structural formula (described in the specification) and is named as 8-hydroxy-7-(3-hydroxypropyl)-2'-methoxyl-flavone (English name). Aloes are taken as a raw material, and are subjected to extraction with extract, silica-gel column chromatographyand high-performance liquid chromatography, so that the flavonoid compound disclosed by the invention is obtained. Activity tests show that the flavonoid compound has better antibacterial effect; andthe flavonoid compound can effectively inhibit microorganism growth in feed liquid of tobacco and prolongs the quality guarantee period of the feed liquid when applied to quality guarantee of the feedliquid of tobacco.

Description

technical field [0001] The invention belongs to the technical field of phytochemistry, and in particular relates to a flavonoid compound extracted from aloe for the first time, and the compound has good antibacterial activity. Simultaneously, the invention also relates to a preparation method of the compound and an application of the compound in preventing corruption of tobacco material liquid and prolonging shelf life of tobacco material liquid. Background technique [0002] Aloe vera is a perennial evergreen herb belonging to the family Liliaceae. It is native to the Mediterranean Sea and Africa. Because it is easy to grow and is an ornamental plant with both flowers and leaves, it is very popular among the public. According to research, there are more than 300 kinds of wild aloe varieties, and the main aloe varieties with medicinal value are: foreign aloe, Curacao aloe, Cape of Good Hope aloe, Yuanjiang aloe and so on. Aloe is edible, medicinal, cosmetic and ornamental. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/30C07D311/40A24B15/40
CPCA24B15/183A24B15/303A24B15/403
Inventor 张承明孔维松刘欣李晶耿永勤李雪梅米其利王明峰者为周敏杨光宇胡秋芬李干鹏
Owner CHINA TOBACCO YUNNAN IND
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