A kind of active skin care composition and its application
A skin care composition and active technology, applied in the field of cosmetics, can solve the problems of increasing the difficulty of compounding and ensuring the stability of skin care products, and achieve the effects of reducing the apoptosis of fibroblasts, delaying photoaging, and inhibiting melanin. generated effect
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Embodiment 1
[0033] The preparation of embodiment 1 natural pigment ethosome
[0034]
[0035] Preparation of group A natural pigment elastosomes:
[0036] Weigh soybean lecithin, dissolve it with ethanol, then add polyethylene glycol stearate, natural pigment, and mix well; at 400r.min -1 Under certain conditions, use a peristaltic pump to 100μL.min -1 Inject the pre-dissolved aqueous solution of hydroxyethylpiperazineethanesulfonic acid into the ethanol solution at a speed of 10000r.min-1 Stir for 2 minutes to make it homogeneous, and then pass through a 200nm microporous homogeneous membrane to obtain ethanol body fluid, which is cooled and solidified to obtain the natural pigment ethanol body.
[0037] For the preparation steps of the natural pigment elastosomes of groups B-E, refer to the above-mentioned group A.
[0038] The particle size and encapsulation efficiency of the natural pigment elastosomes prepared by the above-mentioned A-E groups were detected respectively, and the...
Embodiment 2
[0042] Example 2 The preparation of the lyophilized powder of rose callus cell suspension culture medium
[0043] (1) Take sterile mountain rose leaves and inoculate them in the solid medium of MS+0.8mg / L 6-BA+1.2mg / L 2,4-D+4g / L agar+25g / L sucrose for callus Induction, the subculture time is 105 days, and the loose light yellow-white callus that has been continuously subcultured for 3 times is selected;
[0044] (2) Take the loose light yellow-white callus obtained in step (1), divide it into mung bean size with sterilized tweezers, and inoculate it with 40ml 1 / 2MS+2.0mg / L 6-BA+0.6mg / L 2,4-D+30g / L sucrose, 100ml Erlenmeyer flask of liquid medium with a pH of 5.8, suspended culture on a constant temperature shaker at 25°C, 100rpm, 12h / d light time, after 15 days of culture, Filter through a 50-mesh cell sieve and take the filtrate, then filter the filtrate through a 80-mesh cell sieve, take the filtrate, sterilize it, and freeze-dry it.
[0045] The preparation of embodiment ...
Embodiment 3
[0048] The preparation of embodiment 3 skin care lotion:
[0049] Dissolve palmatine and shellac acid in water, stir while heating until the two are completely dissolved, cool to room temperature, add ethylhexylglycerin, L-ascorbyl palmitate, cysteinyl glycine, The freeze-dried powder of glycyrrhetinic acid, natural pigment ethotoplast and mountain rose callus cell suspension culture fluid is stirred evenly and filled to obtain the product.
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