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Drug for inhibiting serum amyloid proteins (SAP)

A technology of serum starch and medicine, applied in the field of biomedicine

Inactive Publication Date: 2018-05-18
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the specific mechanism and target of benserazide hydrochloride against atherosclerosis have not been reported yet.

Method used

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  • Drug for inhibiting serum amyloid proteins (SAP)
  • Drug for inhibiting serum amyloid proteins (SAP)
  • Drug for inhibiting serum amyloid proteins (SAP)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0021] 1. Extract cellular mRNA and do QPCR experiment

[0022] Collect the cells, wash twice with ice-cold 0.01M PBS, add 1mL Trizol to each well, and collect the cell suspension in RNase-free 1.5ml EP tubes. Add 200 μL of chloroform to each tube, shake vigorously for 30 seconds, let stand at room temperature for 5 minutes, and centrifuge at 12,000 rpm at 4°C for 10 minutes. Pipette the upper aqueous phase into a new EP tube, add an equal volume of ice-cold isopropanol, mix gently, invert 6-8 times, and stand at -20°C for 10 minutes. Centrifuge at 12000 rpm for 10 min at 4°C to collect the RNA precipitate. Wash twice with 75% ice-cold ethanol, and dry in an ultra-clean table. Add about 20 μL of DEPC water to dissolve, measure the RNA concentration, and then perform reverse transcription according to the reverse transcription kit to obtain cDNA of each group. Finally, perform QPCR according to the SYBR Green kit, and process the data. The result is as figure 1 .

[0023]...

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PUM

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Abstract

The invention studies the inhibitory effect of benserazide hydrochloride on SAP expression through constructing an SAP overexpressed cell model; results show that the benserazide hydrochloride can significantly inhibit the SAP expression quantity of SAP-overexpressed macrophages. In addition, the results not only show that the SAP over-expression can cause the increase in the expression quantity of inflammatory factors TNF-alpha, and a significant difference exists in comparison with a control group, but also show that the SAP over-expression quantity can cause the over-expression of other inflammatory factors such as the TNF-alpha; although the specific effects of SAP in atherosclerosis are unclear, the study can provide a new idea and basis for further clarification of the specific effects and mechanisms of the SAP. In conclusion, the benserazide hydrochloride can significantly inhibit the SAP and TNF-alpha expression quantity of the SAP-overexpressed macrophages, which shows that the benserazide hydrochloride has good potential for improving the atherosclerosis, and a foundation is laid for further study on specific mechanisms and targets of the benzylhydrazine hydrochloride against the atherosclerosis.

Description

technical field [0001] The invention relates to a medicine for inhibiting serum amyloid P, which belongs to the technical field of biomedicine. Background technique [0002] Atherosclerosis (AS) is a common disease that seriously endangers human health and is the main pathological basis of ischemic cardiovascular and cerebrovascular diseases such as coronary heart disease, cerebrovascular disease and thromboembolic disease. There is lipid deposition in the intima and subintima of the arterial wall and its branches, and at the same time, the smooth muscle cells in the middle layer migrate to the subintimal hyperplasia, which thickens the intima and forms yellow or grayish-yellow plaques like atheroma. Inflammation runs through the whole process of occurrence and development of atherosclerosis, and as the common basis of many pathophysiological changes in the process of atherosclerosis, it has received increasing attention and recognition. So far, the pathogenesis of AS has n...

Claims

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Application Information

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IPC IPC(8): A61K31/165A61P9/10
Inventor 何书英杨薇秦淑杰
Owner CHINA PHARM UNIV
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