Device and method for increasing fertility rate of zebra fishes

A zebrafish, fertilization rate technology, applied in fish farming, medical science, climate change adaptation, etc., can solve the problem of low zebrafish fertilization rate, and achieve the effects of simple structure, prevention of swallowing, and low cost

Inactive Publication Date: 2018-05-22
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the problem of low fertilization rate of zebrafish in traditional breeding methods, the present...

Method used

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  • Device and method for increasing fertility rate of zebra fishes
  • Device and method for increasing fertility rate of zebra fishes

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) mature female and male zebrafish are cultured in the upper casing of the above-mentioned device for improving the fertilization rate of zebrafish, and the cultivation water in the cultivation casing contains estrogen endocrine disruptors with a concentration of 5ng / L. During cultivation, each The female zebrafish is injected intraperitoneally with 50 μg / 100g of melatonin every day, and the ventilation mechanism is turned on. The temperature of the water body in the device is 25°C, and the pH value of the water quality is slightly alkaline and controlled at 7;

[0033] (2) The next morning, the female and male zebrafish chased each other, and the female zebrafish laid eggs;

[0034] (3) After the eggs are fertilized, they sink into the lower box through the sieve holes;

[0035] (4) The experimental culture period is one month, and the fertilization rate of zebrafish is counted.

Embodiment 2

[0037] (1) Cultivate mature female and male zebrafish in the upper casing of the device for improving the fertilization rate of zebrafish. The culture water in the cultivation casing contains estrogen endocrine disruptors with a concentration of 10ng / L. During cultivation, each The female zebrafish is injected intraperitoneally with 60 μg / 100g of melatonin every day, and the ventilation mechanism is turned on. The temperature of the water body in the device is 28°C, and the pH value of the water quality is controlled at 8;

[0038] (2) The next morning, the female and male zebrafish chased each other, and the female zebrafish laid eggs;

[0039] (3) After the eggs are fertilized, they sink into the lower box through the sieve holes;

[0040] (4) The experimental culture period is one month, and the fertilization rate of zebrafish is counted.

Embodiment 3

[0042] (1) mature female and male zebrafish are cultured in the upper casing of the above-mentioned device for improving the fertilization rate of zebrafish, and the cultivation water in the cultivation casing contains estrogen endocrine disruptors with a concentration of 15ng / L. During cultivation, each The female zebrafish is injected intraperitoneally with 40μg / 100g of melatonin every day, and the ventilation mechanism is turned on. The temperature of the water body in the device is 31°C, and the pH value of the water quality is controlled at 7.5.

[0043] (2) The next morning, the female and male zebrafish chased each other, and the female zebrafish laid eggs;

[0044] (3) After the eggs are fertilized, they sink into the lower box through the sieve holes;

[0045] (4) The experimental culture period is one month, and the fertilization rate of zebrafish is counted.

[0046] Blank control group

[0047] (1) Breed mature female and male zebrafish in the upper box of the ab...

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PUM

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Abstract

The invention relates to the technical field of aquaculture and in particular relates to a device and method for increasing the fertility rate of zebra fishes. The device comprises a culture box; theculture box is partitioned by a horizontally-arranged partition plate to form an upper box body and a lower box body; sieve pores are formed in the partition plate; and the diameters of the sieve pores are larger than the external diameters of spawns of the zebra fishes and are smaller than the external diameters of the zebra fishes. The device is simple in structure, low in cost and convenient touse and maintain; and melatonin is capable of increasing the fertility rate of the zebra fishes by maintaining the expression of a key fertilization protein Ovastacin and a folate receptor protein, so that a theoretical basis is provided for increasing the reproduction rate of fishes.

Description

technical field [0001] The invention relates to the technical field of aquaculture, in particular to a device and method for improving the fertilization rate of zebrafish. Background technique [0002] Zebrafish is a subtropical freshwater fish. Due to its small size, periodic spawning, short spawning cycle, in vitro fertilization, and in vitro development, it has high scientific research value and is a model animal. The body of the zebrafish is elongated and slightly spindle-shaped, the head is small and pointed, and the snout is short. The whole body is covered with many dark blue vertical stripes like zebras, and the lines arranged alternately with silvery white or golden yellow vertical stripes are more orderly. When swimming in groups in the aquarium, it looks like a herd of zebras running on the African grasslands, so it is called zebrafish. Zebrafish and human genes have a high degree of homology of 87%, and their advantages as a model organism are very prominent, wh...

Claims

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Application Information

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IPC IPC(8): A01K63/00A01K63/04A01K61/10A61D7/00
CPCA01K61/10A01K63/003A01K63/042A61D7/00Y02A40/81
Inventor 代小新牟毅赵波高阳储张杰白琛华王通孙凯璐游佳昀黎媛
Owner ZHEJIANG OCEAN UNIV
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