Capillary electrophoresis nucleic acid analysis method and system

Abstract

The invention relates to the technical field of biology and provides a capillary electrophoresis nucleic acid analysis method and system. The method includes: acquiring an original fluorescence spectrum, obtained by detection of a nucleic acid sample, acquired by a capillary electrophoresis nucleic acid analysis device; subjecting the original fluorescence spectrum to second-order derivation to obtain second-order derivation data, and calculating a minimum value of the second-order derivation data; dividing the second-order derivation data by the minimum value of the second-order derivation data to obtain new second-order derivation data; subjecting a negative value of the new second-order derivation data to zero setting to obtain second-order derivative spectrum data; subjecting the second-order derivative spectrum data to normalization processing to obtain a derivative correction spectrum, and performing electrophoresis nucleic acid analysis based on the derivative correction spectrum to solve the problem of 8-color fluorescent dye spectral overlapping in the prior art, so that nucleic acid fragment electrophoresis signal analysis is benefited.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a capillary electrophoresis nucleic acid analysis method and system. Background technique [0002] At this stage, the original fluorescence spectra of fluorescent dyes widely used for labeling nucleic acid molecules are broad spectrums in the visible light band, which makes spectral overlaps in the fluorescence spectra detected by CCD after light splitting by a spectrometer. At present, the maximum number of capillary electrophoresis fluorescent dyes is 6 colors. When the number of fluorescent dyes reaches 8 colors, the original fluorescence spectra of each dye will overlap seriously, resulting in serious pull peaks in the electrophoretic signal, which is not conducive to the electrophoresis of nucleic acid fragments. Analysis of the signal. At the same time, due to the serious overlapping of the fluorescence spectra of the eight fluorescent labeling dyes, the resolution of the beam...

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Problems solved by technology

[0004] In view of this, the object of the present invention is to provide a capillary electrophoresis nucleic acid analysis method and system to solve the technical problem in the prior art that the spectra of 8-color fluorescent dyes overlap, which is not conducive to the analysis of nucleic acid fragment electrophoresis signals

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