Method for establishing neutrophil-type nasal polyp mouse model

A technology of neutrophils and establishment methods, applied in the establishment of neutrophil-type nasal polyp mouse models, can solve the problem of lack of neutrophil-type nasal polyp mouse animal models, etc. Achieve good stability and good operation controllability

Inactive Publication Date: 2018-07-06
王水斌
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Aiming at the defects in the prior art, the present invention aims to solve the problem of lack of a safe and reliable neutrophil nasal polyposis mouse animal model at home and abroad

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  • Method for establishing neutrophil-type nasal polyp mouse model
  • Method for establishing neutrophil-type nasal polyp mouse model
  • Method for establishing neutrophil-type nasal polyp mouse model

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Embodiment Construction

[0032] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the drawings in the embodiments of the present invention. Apparently, the described embodiments are only some of the embodiments of the present invention, but not all of them. All other embodiments obtained by persons of ordinary skill in the art based on the embodiments of the present invention belong to the protection scope of the present invention.

[0033] 1. Main reagents and materials: C57BL / 6J SPF mice (Experimental Animal Center, Zhongnan Hospital, Wuhan University), hematoxylin and eosin staining solution (purchased from Shanghai Biyuntian Biotechnology Co., Ltd.), periodic acid Schiff staining (purchased from Fuzhou Maixin Biotechnology Co., Ltd.), lipopolysaccharide LPS (Escherichia coli Sigma Germany), anti-mouse MPO, OMP, CD68, TLR4 (Abcam, Cambridge, UK), horseradish peroxidase-labeled streptavidin -Biotin complex second...

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Abstract

The invention provides a method for establishing a neutrophil-type nasal polyp mouse model. The method comprises a step of dropping lipopolysaccharide (LPS) into the nasal cavity of a mouse. By virtueof multiple detection methods and multiple detection indexes such as haematoxylin-eosin staining, periodic acid-Schiff reaction, immunohistochemistry, ELISA (Enzyme-Linked Immuno Sorbent Assay), smell experiments and the like, the method provided by the invention fully proves that the neutrophil-type nasal polyp mouse model is successfully and reliably established. Pathological features of the nasal polyp mouse model accord with characteristic symptoms and symptomatic changes of neutrophil-type nasal polyp patients, namely according with pathological processes of the human neutrophil-type nasal polyp mouse models. The mouse model is excellent in operating controllability and stability and can be applied to researching occurrence and development mechanisms and control measures of the neutrophil-type nasal polyp.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a method for establishing a neutrophil-type nasal polyp mouse model and the application of LPS in establishing the neutrophil-type nasal polyp mouse model. Background technique [0002] Nasal polyps are one of the common and frequently-occurring diseases of the upper respiratory tract, which not only seriously affects the quality of life of patients, but also brings a heavy economic burden to families and society. According to the main inflammatory cells, nasal polyps can be divided into two types, mainly eosinocytes and neutrophils, both of which have different immunohistopathological and clinical features. The former is the main type of nasal polyps in Western countries such as Europe and the United States, while the latter is the main type of nasal polyposis in Asian patients. The cause of nasal polyps is not well understood, but various factors such as viruses, bacteri...

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): A61K31/739A61P11/02
CPCA61K31/739
Inventor王水斌
Owner王水斌