Application of Colloidal Gold in Preparation of Artificial Cerebrospinal Fluid Reagent

A technology of artificial cerebrospinal fluid and colloidal gold, which is applied in the field of medical artificial cerebrospinal fluid preparation, can solve the problems of disrupting the circulation stability of cerebrospinal fluid, nerve cell edema and degeneration, etc., and achieve the effect of convenient and quick operation, simple raw materials, and improvement of cerebral edema

Active Publication Date: 2020-12-18
THE FIRST AFFILIATED HOSPITAL OF ARMY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the artificial cerebrospinal fluid currently used in clinical practice does not contain cellular components, and its colloid osmotic pressure is far from the actual cerebrospinal fluid. Its application may disrupt the circulatory stability of cerebrospinal fluid and cause secondary edema and degeneration of nerve cells.

Method used

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  • Application of Colloidal Gold in Preparation of Artificial Cerebrospinal Fluid Reagent
  • Application of Colloidal Gold in Preparation of Artificial Cerebrospinal Fluid Reagent
  • Application of Colloidal Gold in Preparation of Artificial Cerebrospinal Fluid Reagent

Examples

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Embodiment 1

[0026] A preparation method of an artificial cerebrospinal fluid preparation containing colloidal gold, comprising the following steps: at 25° C., dissolving chloroauric acid crystals in distilled water to prepare 100 ml of a 0.01% chloroauric acid solution, heating to 95° C., maintaining 15 minutes; quickly add 25 μl of 0.1% trisodium citrate aqueous solution, stir for 30 minutes while heating, and set the stirring speed to 200 rpm to obtain a colloidal gold solution; Centrifuge at °C, discard the supernatant, dissolve in 100 ml of distilled water, centrifuge at 8000 rpm at 4 °C, and repeat three times. Then add sodium chloride 0.6279g, potassium chloride 0.0216g, calcium chloride 0.0353g, sodium bicarbonate 0.1932g, magnesium chloride 0.0488g, glucose 0.06g, disodium hydrogen phosphate 0.0358g. Finally, make up to 100ml with double distilled water.

[0027] Configured artificial cerebrospinal fluid containing 95% O 2 , 5%CO 2 The mixed gas can be saturated, and the pH val...

Embodiment 2

[0029] Male Sprague-Dawley rats (body weight 250-300 g) with experimental subarachnoid hemorrhage were randomly divided into colloidal gold-containing artificial cerebrospinal fluid irrigation group (n=12) and control group (n=12). After subarachnoid hemorrhage, cerebrospinal fluid irrigation was performed for 24 hours, specifically: the artificial cerebrospinal fluid containing colloidal gold was slowly pumped into the lateral ventricle (the rate was 0.5ml / h), and the lumbar cistern was drained. On the 7th day after subarachnoid hemorrhage, two groups of animals were sacrificed to observe the changes in the volume of subarachnoid hematoma at the base of the skull and brain water content (detection of cerebral edema). The experimental results are as follows: After dissection of the rat brain tissue, the general observation found that the amount of hematoma at the base of the skull was significantly reduced in the new artificial cerebrospinal fluid irrigation group after 7 days ...

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Abstract

The invention provides new application of colloidal gold to preparation of an artificial cerebrospinal fluid reagent. The colloidal gold comprises colloidal gold particles, wherein the particle size range is 5 to 25 [mu]m, the average particle size is 13.4+ / -3.6 [mu]m, the colloidal gold concentration is 150 to 400 mg / L, and the average concentration is 232.4+ / -38.3 mg / L. The colloid osmotic pressure and the cell environment of human cerebrospinal fluid can be simulated, and novel artificial cerebrospinal fluid according with the physiological characteristics is created.

Description

technical field [0001] The invention belongs to the field of preparation of medical artificial cerebrospinal fluid, in particular to the application of colloidal gold in the preparation of artificial cerebrospinal fluid. Background technique [0002] Cerebrospinal fluid (Cerebro-Spinal Fluid, CSF) is a colorless and transparent liquid that fills the ventricles, the subarachnoid space and the central canal of the spinal cord. Cerebrospinal fluid is produced by the choroid plexus in the ventricles and finally permeates through the arachnoid granules in the subarachnoid space on the dorsal side of the brain into the superior sagittal sinus and returns to the veins. Cerebrospinal fluid is similar in nature to plasma and lymph, is slightly viscous, and belongs to extracellular fluid. Normal cerebrospinal fluid has a certain osmotic pressure, mainly composed of crystal osmotic pressure and colloid osmotic pressure, which plays an important role in maintaining the relative stabili...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K33/242A61K33/42A61P7/08A61K33/14A61K33/06A61K33/00A61K31/7004
CPCA61K31/7004A61K33/00A61K33/06A61K33/14A61K33/24A61K33/42A61K2300/00
Inventor 谭亮蒋周阳缪洪平牛胤王菊刘昕冯华
Owner THE FIRST AFFILIATED HOSPITAL OF ARMY MEDICAL UNIV
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