Application of Colloidal Gold in Preparation of Artificial Cerebrospinal Fluid Reagent
A technology of artificial cerebrospinal fluid and colloidal gold, which is applied in the field of medical artificial cerebrospinal fluid preparation, can solve the problems of disrupting the circulation stability of cerebrospinal fluid, nerve cell edema and degeneration, etc., and achieve the effect of convenient and quick operation, simple raw materials, and improvement of cerebral edema
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Embodiment 1
[0026] A preparation method of an artificial cerebrospinal fluid preparation containing colloidal gold, comprising the following steps: at 25° C., dissolving chloroauric acid crystals in distilled water to prepare 100 ml of a 0.01% chloroauric acid solution, heating to 95° C., maintaining 15 minutes; quickly add 25 μl of 0.1% trisodium citrate aqueous solution, stir for 30 minutes while heating, and set the stirring speed to 200 rpm to obtain a colloidal gold solution; Centrifuge at °C, discard the supernatant, dissolve in 100 ml of distilled water, centrifuge at 8000 rpm at 4 °C, and repeat three times. Then add sodium chloride 0.6279g, potassium chloride 0.0216g, calcium chloride 0.0353g, sodium bicarbonate 0.1932g, magnesium chloride 0.0488g, glucose 0.06g, disodium hydrogen phosphate 0.0358g. Finally, make up to 100ml with double distilled water.
[0027] Configured artificial cerebrospinal fluid containing 95% O 2 , 5%CO 2 The mixed gas can be saturated, and the pH val...
Embodiment 2
[0029] Male Sprague-Dawley rats (body weight 250-300 g) with experimental subarachnoid hemorrhage were randomly divided into colloidal gold-containing artificial cerebrospinal fluid irrigation group (n=12) and control group (n=12). After subarachnoid hemorrhage, cerebrospinal fluid irrigation was performed for 24 hours, specifically: the artificial cerebrospinal fluid containing colloidal gold was slowly pumped into the lateral ventricle (the rate was 0.5ml / h), and the lumbar cistern was drained. On the 7th day after subarachnoid hemorrhage, two groups of animals were sacrificed to observe the changes in the volume of subarachnoid hematoma at the base of the skull and brain water content (detection of cerebral edema). The experimental results are as follows: After dissection of the rat brain tissue, the general observation found that the amount of hematoma at the base of the skull was significantly reduced in the new artificial cerebrospinal fluid irrigation group after 7 days ...
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