A portable lensless multispectral microscopic imaging system and method

Abstract

The invention discloses a portable lensless multi-spectrum microscopic imaging system and method. The system includes a light source lighting module, a moving stage and an image sensor arranged sequentially along the optical path. components, a color combining prism, and an exit hole. The outgoing light of multiple light-emitting elements enters the color combining prism from different incident directions and exits, and then passes through the exit hole to the mobile stage; between the mobile stage and the image sensor There is a calibration plate with a calibration pattern and a transparent area; the image sensor is used to acquire the image data of the calibration pattern to calibrate the wavelength of the light-emitting element, and is also used to obtain the sample to be inspected on the mobile stage through the transparent area image data. The portable multispectral lensless microscopic imaging system of the present invention has a simple structure, omits an imaging lens and a precision spectral device, and is suitable for portable detection of biological tissues and cells under field conditions.

Description

technical field [0001] The invention relates to the field of microscopic imaging, in particular to a lensless multispectral microscopic imaging system and method. Background technique [0002] One of the commonly used detection methods is to use a biological microscope to visually detect the collected biochemical samples. Most of the existing microscopes are based on the optical microscope structure of the microscopic objective lens. The low magnification optical microscope is relatively cheap, but the resolution is limited, usually 2um; the high magnification optical microscope can reach the optical resolution of 300nm, such as phase contrast Microscopes, differential interference phase contrast microscopes, fluorescence microscopes, and laser confocal microscopes have promoted the improvement of life science research, and provided powerful tools for disease diagnosis, especially early diagnosis of major malignant diseases, with higher resolution and imaging quality. Imagi...

AI Technical Summary

Problems solved by technology

Most of the existing microscopes are based on the optical microscope structure of the microscopic objective lens. The low magnification optical microscope is relatively cheap, but the resolution is limited, usually 2um; the high magnification optical microscope can reach the optical resolution of 300nm, such as phase contrast Microscopes, differential interference phase contrast microscopes, fluorescence microscopes, and laser confocal microscopes have promoted the improvement of life science research, and provided powerful tools for disease diagnosis, especially early diagnosis of major malignant diseases, with higher resolution and imaging quality. Imaging evidence, but usually expensive, bulky, and cumbersome, which is not conducive to field use

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