Pinus massoniana mycorrhizal seedling culture method

A technology of masson pine and mycorrhizalization, applied in botany equipment and methods, horticulture, plant cultivation, etc., can solve the problems of cumbersome root cutting process, low survival rate of mycorrhizal seedlings, low infection rate, etc., and achieve root cutting process Simple, ideal inoculation effect, guaranteed effect of infection rate

Active Publication Date: 2018-08-07
GUIZHOU UNIV
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Problems solved by technology

[0004] In order to solve the above-mentioned technical problems, the present invention provides a method for raising seedlings of P. massoniana mycorrhized seedlings. The method makes the mycorrhizal seedlings of P. massoniana more convenient and simple, and solves the root cutting process in the process of mycorrhizal seedlings of P. massoniana target species Problems such as cumbersome, low survival rate of mycorrhizal seedlings, and low infection rate

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  • Pinus massoniana mycorrhizal seedling culture method

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Embodiment Construction

[0019] In order to make the purpose, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0020] Embodiments of the present invention: a method for cultivating pine mycorrhized seedlings comprises the following steps:

[0021] (1) Select the seeds with better quality, select them with water, sterilize them with 5% potassium permanganate for 2 hours, remove them, put them in a beaker, cover them with a film seal and let them stand for 30 minutes, rinse them with sterile water for 3 times and place them in The initial temperature is 45°C in sterile water, and then placed in an artificial climate box at a temperature of about 25°C to soak the seeds for 24 hours, and the water is changed every 6 hours during the process. Germinate in the box, and sow after the seeds are white.

[0022] (2) Sterilize the seedling-growing substrate with a h...

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Abstract

The invention discloses a pinus massoniana mycorrhizal seedling culture method. The method comprises five steps including seed disinfection and germination, nursery substrate disinfection, strain acquisition and bacterial liquid preparation, germchit root cutting treatment and bacterial liquid inoculation, and transplant management. Through the method of the invention, mycorrhizal seedlings of target strains can be easily obtained, and the infection rate thereof are ensured; the operation process is simple and convenient, the inoculation effect is very satisfactory, the infection rate is up to95% or above, the root cutting process is simple, convenient and quick, and the damage to the seedlings is small; a germination box and a mesh partition used in the invention can be recycled, being economical and environmentally friendly.

Description

technical field [0001] The invention relates to a method for growing seedlings of masson pine mycorrhized seedlings, belonging to the technical field of mycorrhized seedlings. Background technique [0002] Masson pine (Pinus massoniana Lamb.) is a native tree species with the largest planting area, the widest distribution, and the highest degree of comprehensive utilization of the whole tree among conifer species in my country. It is also one of the most important industrial timber species in the south, and has important economic and ecological values. It belongs to typical ectomycorrhizal tree species. Ectomycorrhizae can promote the absorption of mineral nutrients by plants, enhance the stress resistance of plants, and then promote their growth. But in the actual seedling raising work, it is very difficult to obtain the mycorrhized Pinus massoniana seedlings of the target strain. [0003] Ectomycorrhiza is a symbiont formed by ectomycorrhizal fungi and young roots of Pinu...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G17/00A01G18/10
CPCA01G17/005A01G18/10
Inventor 丁贵杰陈后英刘海燕李快芬
Owner GUIZHOU UNIV
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