Pressure-controllable small cell culture device

Abstract

The invention discloses a pressure-controllable small cell culture device. The pressure-controllable small cell culture device comprises a culture box, an adjustable air pump, a first adjustable valve, second adjustable valves, pressure meters and a third adjustable valve, wherein the culture box comprises a box body, a plurality of culture spaces arranged in the box body and a box cover arrangedat an opening of the box body; the adjustable air pump is connected with the first adjustable valve to be correspondingly connected into the multiple culture spaces one to one through a plurality of feeding pipes, all the feeding pipes are externally connected with branch pipes, and the second adjustable valves are arranged on the branch pipes; all the culture spaces are externally connected withthe pressure meters and are externally connected through a plurality of discharging pipes, and the multiple discharging pipes are collected and connected with the third adjustable valve. According tothe pressure-controllable small cell culture device, different-pressure control experiments can be carried out in the multiple culture spaces, experimental environments of the multiple culture spacescan be kept coincident, the accurate experimental result is obtained, and the influence of high pressure on the glaucoma morbidity can be better researched.

Description

technical field [0001] The invention relates to the field of medical experiments, in particular to a pressure-controllable small cell culture device. Background technique [0002] Glaucoma is a neurodegenerative disease characterized by irreversible damage to retinal ganglion cells (RGCs), and is the leading ophthalmic disease with the highest blinding rate in the world. There are about 64.3 million glaucoma patients in the 40-80 age group worldwide, and it is expected to reach 76 million and 111.8 million by 2020 and 2040, respectively. Elevated pathological intraocular pressure is an important factor leading to the occurrence and progression of glaucoma. Simulating the impact of high pressure on related cells in vitro is an important part of the research on the pathogenesis of glaucoma. [0003] In vitro pressure culture of cells is a cell culture technique that applies different pressures to cells during in vitro culture to study the effects of pressure on cell biologica...

AI Technical Summary

Problems solved by technology

[0004] However, in the existing pressure culture, the technology is relatively simple, and generally directly pump air into the culture bottle with a balloon and a manometer in conjunction with a three-way tube. Circulation, with the progress of cell culture, the CO2 concentration and pH value in the culture bottle will change greatly, which cannot completely simulate the situation in the body; ②The operation is more complicated, and some laboratories transform animals by themselves in order to create suitable conditions Pressurized chambers are used to make culture instruments, which consumes a lot of time and energy

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