A circulating miRNA marker related to auxiliary diagnosis of papillary carcinoma of thyroid and application thereof

A technique for papillary carcinoma and auxiliary diagnosis, which is applied in the fields of genetic engineering and oncology, can solve the problems of incompletely consistent research results, and achieve the effects of high sensitivity and specificity, minimally invasive and easy access

Active Publication Date: 2018-10-09
朱伟
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to differences in research methods and included populations, the results of the studies were not completely consistent.

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  • A circulating miRNA marker related to auxiliary diagnosis of papillary carcinoma of thyroid and application thereof
  • A circulating miRNA marker related to auxiliary diagnosis of papillary carcinoma of thyroid and application thereof
  • A circulating miRNA marker related to auxiliary diagnosis of papillary carcinoma of thyroid and application thereof

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Embodiment Construction

[0043] The inventor collected a large number of venous serum and plasma samples from the First Affiliated Hospital of Nanjing Medical University from 2014 to 2015 from patients with papillary thyroid carcinoma and normal physical examination population. Samples from 29 cases of nodular goiter, 29 cases of nodular goiter and 131 cases of normal controls were used as experimental samples for the initial screening of Exiqon miRNAqPCR panel chip and subsequent series of qRT-PCR verification. At the same time, 17 cases of papillary thyroid carcinoma tissues and 17 cases of adjacent tissues were collected to verify the expression of plasma miRNA markers in the tissues, and 23 cases of papillary thyroid carcinoma tissues and 23 cases of adjacent tissues were collected to verify the expression of serum miRNA markers. expression in tissues. The selected patients' serum and plasma samples were all from newly diagnosed patients who had not undergone surgery, radiotherapy and chemotherapy...

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Abstract

The present invention discloses a circulating miRNA marker related to auxiliary diagnosis of papillary carcinoma of thyroid and application thereof. The marker is one or more selected from plasma markers miR-346, miR-10a-5p and miR-34a-5p and serum markers miR-25-3p, miR-296-5p and miR-92a-3p. As a novel biomarker, circulating miRNA has the characteristics of good stability, easy preparation through minimally invasive operation, high sensitivity and high specificity, and development and utilization of molecular markers will provide new directions for the diagnosis and further treatment of various diseases including tumors. The circulating miRNA marker having a clinical diagnosis potential for papillary carcinoma of thyroid is provided by the invention in a more targeted manner. Researchesconfirm reliability and reproducibility of this group of miRNAs as non-invasive markers for diagnosis of papillary carcinoma of thyroid.

Description

technical field [0001] The invention belongs to the fields of genetic engineering and oncology, and relates to a circulating miRNA marker related to auxiliary diagnosis of thyroid papillary carcinoma and its application. Background technique [0002] Thyroid cancer (Thyroid Cancer) originates from thyroid tissue and is one of the most common malignant tumors in the endocrine system. The global incidence has nearly tripled in the past 30 years. Among them, Papillary Thyroid Carcinoma (PTC) is the most common subtype, accounting for about 80-85% of thyroid cancers. Surgical treatment is currently the first choice and main treatment for papillary thyroid carcinoma, and can achieve the goal of curing early papillary thyroid carcinoma patients. The rate is still low, and it is easy to miss the best opportunity for surgical treatment. At present, ultrasound (ultrasound imaging) and fine-needle aspiration biopsy (Fine-Needle Aspiration biopsy, FNA) are relatively recommended diag...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/11C12N15/113
Inventor 朱伟周鑫邹璇蒋琳朱军陈旭峰吕金如王治砚
Owner 朱伟
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