Zirconia microbead rare blood type detection reagent card and preparation method thereof

Abstract

The invention relates to a zirconia (ZrO2) microsphere rare blood-type detection reagent card and a preparation method thereof. The reagent card contains a zirconia microsphere microcolumn tube of a monoclonal anti-K antibody containing IgM property, a zirconia microsphere microcolumn tube of a monoclonal anti-k antibody containing IgM property, a zirconia microsphere microcolumn tube of a monoclonal anti-S antibody containing IgM property, a zirconia microsphere microcolumn tube of a monoclonal anti-s antibody containing IgM property, a zirconia microsphere microcolumn tube of a monoclonal anti-M antibody containing IgM property, a zirconia microsphere microcolumn tube of a monoclonal anti-N antibody containing IgM property, a zirconia microsphere microcolumn tube of a monoclonal anti-Jkaantibody containing IgM property, and a zirconia microsphere microcolumn tube of a monoclonal anti-Jkb antibody containing IgM property. The rare blood-type detection reagent card has the advantagesof high sensitivity, good specificity, and stable quality.

Description

technical field [0001] The invention relates to the field of medical detection, in particular to a zirconia microbead rare blood type detection reagent card and a preparation method thereof. Background technique [0002] In 1946, in the first report on the application of the direct antiglobulin test (DAT), Coombs et al. described an antibody with a new specificity, originally called anti-K or KEL1, which was produced with the antibody The red blood cells of the patient's husband and two children reacted with 7% of the random blood samples. Three years later, Levine et al. described anti-Cellano, which corresponds to anti-K. Since k has been used to represent the allele of K, the symbol k represents the product of this gene. K and k are the products of co-dominant alleles, which has been confirmed by many family studies. [0003] In 1947, Walsh and Montgomery discovered the anti-S alloantibody, which can detect an antigen associated with MN. Sanger et al. tested 190 Britis...

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Problems solved by technology

However, Sephadex will be affected by changes in temperature, salt concentration, osmotic pressure, and pH. The pore size of the particles will change, and the space outside the bed will also change, which will affect the results of centrifugal chromatography.

Because it is a gel-like particle, its physical strength is poor, and it will cause deformation as the storage period prolongs, and its non-specific adsorption is high, which will affect the test results

In addition, the price is more expensive

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