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Method for detecting standard substance of lanatoside C by using reversed phase high-performance liquid chromatography

A reversed-phase high-performance liquid phase and lanatoside C technology, which is applied in the detection field of lanatoside C standard substance, can solve the problems of no detection method, etc., and achieve the effects of small detection error, simple operation and high response value

Inactive Publication Date: 2018-12-11
SHANGHAI BAINIAN SHIDANDE INSPECTION TECH +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are no literature reports on relevant detection methods at present, so it is necessary to explore a more easy-to-operate and accurate method for the determination of lanatoside C content

Method used

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  • Method for detecting standard substance of lanatoside C by using reversed phase high-performance liquid chromatography
  • Method for detecting standard substance of lanatoside C by using reversed phase high-performance liquid chromatography

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Embodiment 1

[0022] Chromatographic conditions and system adaptability: Agilent ZORBAX SB-C18 (4.6 × 150mm, 5μm) is used for the chromatographic column; the mobile phase is acetonitrile-methanol (22:14) as mobile phase A, water as mobile phase B, gradient elution: 0~20min, 38%A; 20~21min, 38%A~52%A; 21~45min, 52%A; 45~46min, 52%A~38%A; 46~51min, 38%A; flow rate 1.0 ml / min; DAD detector, detection wavelength 220nm; column temperature 30°C; injection volume 5 μl;

[0023] Preparation of the test solution: Accurately weigh 0.8 mg of lanatoside C, accurately add 1 ml of methanol to dissolve, and obtain the product.

[0024] Determination method: Precisely draw 5 μl of the test solution, inject it into a high-performance liquid chromatograph, measure it, and obtain it.

[0025] Results: The measured normalized content of lanatoside C was 99.6%, and the peak shape was symmetrical. For sample and blank control spectra, see figure 1 .

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Abstract

The invention discloses a method for detecting a standard substance of lanatoside C by using a reversed phase high-performance liquid chromatography. The reversed phase high-performance liquid chromatography is adopted for detection, a liquid chromatographic column taking octadecylsilane bonded silica gel as a filler is used, and gradient elution is performed by taking acetonitrile-methanol as a flowing phase A and water as a flowing phase B; the flow speed is 1 ml / min; the column temperature is kept at 30 DEG C; and detection is performed at 220 nm, and the content of a sample is calculated by using an area normalization method. The method has the advantages of simplicity and convenience in operation, high precision and good repeatability and can be used for detecting lanatoside C.

Description

technical field [0001] The invention relates to a detection method of lanatoside C standard substance, more specifically, it relates to a detection method of lanatoside C standard substance by reverse phase high performance liquid chromatography. Background technique [0002] Oratoside C, also known as lanatoside C, has a molecular formula of C49H76O20, a molecular weight of 985.12, and a CAS number of 17575-22-3. It exists in digitalis and other plants and belongs to steroidal components. Its structure is as follows: [0003] [0004] According to literature reports, lanatoside C is suitable for acute and chronic heart failure, atrial fibrillation and paroxysmal supraventricular tachycardia. However, there is no literature report on the relevant detection method at present, so it is necessary to explore a more easy-to-operate and accurate method for the determination of lanatoside C content. Contents of the invention [0005] Aiming at the deficiencies in the prior ar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/89G01N30/02
CPCG01N30/89G01N30/02
Inventor 费文静姜悦沈君子钱勇谢天培
Owner SHANGHAI BAINIAN SHIDANDE INSPECTION TECH
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