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Method for determining content of umbelliferone in traditional Chinese medicine levisticum

An umbelliferone and a detection method technology, applied in the field of medicine, can solve the problems of difficult operation and poor repeatability of the detection method, and achieve the effects of strong practicability, simple operation and high response value.

Inactive Publication Date: 2012-10-10
SHANGHAI BAINIAN SHIDANDE INSPECTION TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing detection methods are not easy to operate and have poor repeatability. Therefore, it is necessary to explore an easier and more accurate method for the determination of the content of umbelliferin in traditional Chinese medicine.

Method used

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  • Method for determining content of umbelliferone in traditional Chinese medicine levisticum
  • Method for determining content of umbelliferone in traditional Chinese medicine levisticum
  • Method for determining content of umbelliferone in traditional Chinese medicine levisticum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Chromatographic conditions and system adaptability: Symmetrix ODS-R (4.6×150mm, 5 μm) is used as a chromatographic column; the mobile phase is acetonitrile:water=18:82; the flow rate is 1ml / min; the detection wavelength is 345nm; Volume 2μl;

[0023] Preparation of the test solution: Take about 0.5g of the dry powder of the lovage medicinal material, accurately weigh it, put it in a stoppered Erlenmeyer flask, add 20ml of methanol precisely, seal it tightly, weigh it, ultrasonically treat it (220W, 40kHz) for 30min, and let it cool , weigh again, make up for the lost weight with methanol, and shake well. Filter with a 0.45μm microporous membrane, that is, too.

[0024] Preparation of standard solution: Accurately weigh 12.3 mg of umbelliferin sample, place it in a 10ml volumetric flask, set the volume to the mark with methanol, and shake well; Make the volume up to the mark, shake well, and you get it.

[0025] Determination method: Precisely draw 2 μl of the sample ...

Embodiment 2

[0028] Chromatographic conditions and system adaptability: Agilent ZORBAX Extend C18 (4.6×150mm, 5 μm) is used for the chromatographic column; the mobile phase is acetonitrile:water=15:85; the flow rate is 1ml / min; the detection wavelength is 345nm; the column temperature is room temperature; the injection volume 20μl;

[0029] Preparation of the test solution: Take about 0.5g of the dry powder of the lovage medicinal material, accurately weigh it, put it in a stoppered Erlenmeyer flask, add 20ml of methanol precisely, seal it tightly, weigh it, ultrasonically treat it (220W, 40kHz) for 30min, and let it cool , weigh again, make up for the lost weight with methanol, and shake well. Filter with a 0.45μm microporous membrane, that is, too.

[0030] Preparation of the reference solution: Accurately weigh 11.9 mg of the umbelliferin sample, place it in a 10ml volumetric flask, set the volume to the mark with methanol, and shake up; Dilute to the mark, shake well, and you're read...

Embodiment 3

[0034] Chromatographic conditions and system adaptability: Symmetrix ODS-R (4.6×250mm, 5μm) is used for the chromatographic column; the mobile phase is acetonitrile:water=20:80; the flow rate is 1ml / min; the detection wavelength is 345nm; the column temperature is 25°C; The volume is 10 μl;

[0035] Preparation of the test solution: Take about 0.5g of the dry powder of the lovage medicinal material, accurately weigh it, put it in a stoppered Erlenmeyer flask, add 20ml of methanol precisely, seal it tightly, weigh it, ultrasonically treat it (220W, 40kHz) for 30min, and let it cool , weigh again, make up for the lost weight with methanol, and shake well. Filter with a 0.45μm microporous membrane, that is, too.

[0036] Preparation of the reference solution: Accurately weigh 10.5 mg of umbelliferin sample, place it in a 10ml volumetric flask, set the volume to the mark with methanol, and shake well; Make the volume up to the mark, shake well, and you get it.

[0037] Determin...

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Abstract

The invention discloses a method for detecting umbelliferone in a traditional Chinese medicine levisticum by reversed phase high-performance liquid chromatography. The method is characterized by comprising the following steps of: carrying out ultrasonic extraction of umbelliferone in the traditional Chinese medicine levisticum by the use of methanol, and preparing a test solution; absorbing the test solution and injecting into high performance liquid chromatograph, determining by reversed phase high-performance liquid chromatography, using C18 column which takes octadecylsilane chemically bonded silica as a filler, using acetonitrile water as a mobile phase, detecting under 345nm at the flow velocity of 1 ml / min with the column temperature of 25 DEG C, carrying out qualitative analysis according to retention time and chromatogram of a sample, and carrying out quantitative analysis according to peak area. The method provided by the invention is simple to operate, has high precision and good reappearance, and can be used to detect umbelliferone in the traditional Chinese medicine levisticum.

Description

technical field [0001] The invention relates to a method for detecting the content of umbelliferin (7-Hydroxycoumarin) in traditional Chinese medicine lovage, belonging to the technical field of medicine. Background technique [0002] The solitary life is the dry root of Angelica pubescens Maxi m.f.biserrata Shan et Yuan. It has the effects of expelling wind and removing dampness, clearing numbness and relieving pain. [0003] Umbellifer, also known as 7-hydroxycoumarin, has the molecular formula: C 9 h 6 o 3 , with a molecular weight of 162.14 and a CAS number of 93-35-6. It is widely found in Umbelliferae, carrots, Rutaceae Rutaceae and other plants. It belongs to coumarin components. Its structure is shown in the figure: [0004] [0005] According to literature reports, umbelliferone has antibacterial, antihypertensive, anticancer and other effects. However, the existing detection methods are not easy to operate and have poor repeatability. Therefore, it is neces...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/14G01N30/60
Inventor 费文静钱勇程萍许纪锋李莉谢天培
Owner SHANGHAI BAINIAN SHIDANDE INSPECTION TECH
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