Method for determining content of umbelliferone in traditional Chinese medicine levisticum

An umbelliferone and a detection method technology, applied in the field of medicine, can solve the problems of difficult operation and poor repeatability of the detection method, and achieve the effects of strong practicability, simple operation and high response value.
CN102721774AInactive Publication Date: 2012-10-10SHANGHAI BAINIAN SHIDANDE INSPECTION TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHANGHAI BAINIAN SHIDANDE INSPECTION TECH
Publication Date
2012-10-10
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for detecting umbelliferone in a traditional Chinese medicine levisticum by reversed phase high-performance liquid chromatography. The method is characterized by comprising the following steps of: carrying out ultrasonic extraction of umbelliferone in the traditional Chinese medicine levisticum by the use of methanol, and preparing a test solution; absorbing the test solution and injecting into high performance liquid chromatograph, determining by reversed phase high-performance liquid chromatography, using C18 column which takes octadecylsilane chemically bonded silica as a filler, using acetonitrile water as a mobile phase, detecting under 345nm at the flow velocity of 1 ml / min with the column temperature of 25 DEG C, carrying out qualitative analysis according to retention time and chromatogram of a sample, and carrying out quantitative analysis according to peak area. The method provided by the invention is simple to operate, has high precision and good reappearance, and can be used to detect umbelliferone in the traditional Chinese medicine levisticum.
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Description

technical field

[0001] The invention relates to a method for detecting the content of umbelliferin (7-Hydroxycoumarin) in traditional Chinese medicine lovage, belonging to the technical field of medicine. Background technique

[0002] The solitary life is the dry root of Angelica pubescens Maxi m.f.biserrata Shan et Yuan. It has the effects of expelling wind and removing dampness, clearing numbness and relieving pain.

[0003] Umbellifer, also known as 7-hydroxycoumarin, has the molecular formula: C 9 h 6 o 3 , with a molecular weight of 162.14 and a CAS number of 93-35-6. It is widely found in Umbelliferae, carrots, Rutaceae Rutaceae and other plants. It belongs to coumarin components. Its structure is shown in the figure:

[0004]

[0005] According to literature reports, umbelliferone has antibacterial, antihypertensive, anticancer and other effects. However, the existing detection methods are not easy to operate and have poor repeatability. Therefore, it is neces...

Claims

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