Method for screening high-nitrogen-use-efficiency rape variety by use of seedling-stage brassica napus culture experiment

Abstract

The invention relates to a method for screening a high-nitrogen-use-efficiency brassica napus variety by use of a seedling-stage brassica napus culture experiment. The method for screening a high-nitrogen-use-efficiency rape variety by use of the seedling-stage brassica napus culture experiment is characterized by comprising the following steps: (1) seed selection; (2) seedling culture; (3) transplantation; (4) collection of calculation indexes: biomass, nitrogen accumulation and a lateral root length; (5) comprehensive judgment on a nitrogen efficiency genotype of a rape to be evaluated by use of the calculation indexes, wherein, after the rape is transplanted and cultured for 21 days and if the total biomass is larger than 0.331 g / plant and the nitrogen accumulation is larger than 7.88 mg / plant, the rape is a high-nitrogen-use-efficiency rape; if the biomass is between 0.290 and 0.331 g / plant and the nitrogen accumulation is between 6.70 and 7.88 mg / plant, the rape is a medium-nitrogen-use-efficiency rape; if the total biomass is smaller than 0.290 g / plant and the nitrogen accumulation is smaller than 6.70 mg / plant, the rape is a low-nitrogen-use-efficiency rape. According to themethod, a high-quality rape germplasm resource screening process is effectively improved, the labor cost is reduced, the screening efficiency of a high-nitrogen-use-efficiency variety is improved, and a rape variety of a high-nitrogen-use-efficiency genotype is screened.

Description

technical field [0001] The invention relates to a method for screening rapeseed varieties with high nitrogen efficiency (evaluation method for nitrogen efficiency of different varieties of Brassica napus) by using a cultivation test of Brassica napus at the seedling stage, and belongs to the field of fertilizer efficiency evaluation and plant variety screening. Background technique [0002] In rapeseed production, excessive nitrogen fertilizer input has led to high planting costs for farmers, low planting benefits, and low enthusiasm for planting farmers. At the same time, the nitrogen use efficiency of rapeseed is low, and excessive nitrogen fertilizer loss has had a negative impact on the environment. Therefore, , screening rapeseed germplasm resources or varieties with high nitrogen efficiency, clarifying the type of nitrogen nutrient use efficiency of rapeseed varieties in the process of rapeseed production, tapping the genetic potential of rapeseed nitrogen absorption and...

AI Technical Summary

Problems solved by technology

[0004] However, because the test process will be affected or limited by many factors, if the test screening cycle is too long, it is difficult to achieve the test principle of local control to achieve the only difference, and the comparative evaluation and screening of large batches of varieties requires a large workload and serious Constraining the development of screening tests

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