Method and system for constructing mouse model for embryo transfer

Abstract

The invention belongs to the technical field of embryo transfer, and discloses a method and system for constructing a mouse model for embryo transfer. The system comprises a cell selection module, anin vitro preservation module, a culture solution formulation module, a microinjection module and a culture module. The method adds high-concentration pyruvic acid to reduce the culture temperature byusing the in vitro preservation module to prevent oocyte aging and prolong the preservation time in vitro; the method preserves mature oocytes of mammals in vitro for up to 42 hours without affectingdevelopmental capacity.

Description

technical field [0001] The invention belongs to the technical field of embryo transfer, and in particular relates to a method and system for constructing a mouse model for embryo transfer. Background technique [0002] Embryo segmentation refers to the technology of mechanically cutting early embryos into 2 equal parts, 4 equal parts or 8 equal parts, etc., and obtaining identical twins or multiple births through transplantation. The main equipment needed for embryo segmentation is a solid microscope and a micromanipulator. When dividing embryos, you should choose well-developed morula or blastocysts, and divide them with a dividing needle or a dividing knife. When dividing blastocysts, pay attention to dividing the inner cell mass equally, otherwise it will affect the recovery and further development of the embryo . However, the existing embryo transfer process has a short storage time in vitro, is prone to aging, and has a low survival rate; at the same time, the existin...

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Problems solved by technology

[0004] (1) The existing embryo transplantation process has a short storage time in vitro, is prone to aging, and has a low survival rate; at the same time, the existing mouse models are difficult to carry out under real disease conditions, which is not conducive to the research of related diseases, and consumes a lot of resources when performing cell selection

[0005] (2) The filling speed of the currently used microneedle filling method is slow, and the required filling time is long, which prolongs the experimental cycle and slows down the experimental process

[0006] (3) The identification method currently used needs to be identified by comparing the spectra of various samples. The operation is complicated, the application range is small, and the sensitivity is low, which cannot meet the requirements of this experiment.

[0007] In the prior art, when extracting male and female mice to provide stable oocyte image information, the obtained image information has poor clarity, which affects post-processing and restricts the development of embryo transfer technology.

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