A method for detecting c-kit gene mutation

Abstract

The invention discloses a c. A method for detecting mutation of kit gene comprising the follow steps: S1, according to 9, 11, 13 and 17 c-Kit Exon DNA Sequences, designing Specific Amplification Primers; S2, PCR amplification of No. 9, 11, 13 and 17 c by using the specific amplification primers designed in step S1; Kit exon, and the PCR amplified products being digested by enzymes; S3, carrying out cyclic amplification on that enzymatic hydrolysate according to the sequence primer to obtain a Sanger fragment; S4, using the Sanger fragment obtained in the step S3, analyzing No.9, 11, 13 and 17c-Kit exon DNA sequence information through an automatic gene instrument, comparing with wild genotype to find out the mutation site. The invention designs the specific amplification primer, shortensthe length of the primer, improves the specific amplification sensitivity, and intuitively sees whether the target gene amplified by PCR is qualified through the specific amplification primer, therebydeciding whether to carry out the next step and avoiding wasting the reagent.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a c-kit gene mutation detection method. Background technique [0002] Gastrointestinal stromal tumor (GIST) is the most common mesenchymal tumor of the digestive tract. Most GISTs express Kit protein (CD117) encoded by c-kit gene. At the molecular level, most GISTs have c-kit gene mutations, which lead to the activation of Kit protein without the participation of ligand SCF to stimulate the continuous proliferation of tumor cells and the loss of control of anti-apoptotic signals. [0003] The mutation rate of c-kit gene in GIST is about 90%, and the mutation forms are various. Among them, the mutation located in the Lys550-Val560 segment of exon 11 is the most common (about 70-80%), and the 6-base repeat mutation located in the Ala502-Tyr503 segment of exon 9 accounts for about 5-10%. Clinical studies have shown that the mutation of c-Kit gene in GIST is related t...

AI Technical Summary

Problems solved by technology

In addition, patients with exon 17 D816V mutation are resistant to imatinib

Images