Mettl3-knockout spermatogenesis disorder animal model and building method thereof

A technology for spermatogenesis disorders and animal models, applied in biochemical equipment and methods, other methods of inserting foreign genetic materials, using vectors to introduce foreign genetic materials, etc., can solve problems such as functional redundancy

Inactive Publication Date: 2019-03-05
CENT FOR EXCELLENCE IN MOLECULAR CELL SCI CHINESE ACAD OF SCI
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Problems solved by technology

Studies at the cellular level have shown that both METTL3 and METTL14 have methyltransferase activity, and there is a certain functional redundancy between them

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  • Mettl3-knockout spermatogenesis disorder animal model and building method thereof
  • Mettl3-knockout spermatogenesis disorder animal model and building method thereof
  • Mettl3-knockout spermatogenesis disorder animal model and building method thereof

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Embodiment Construction

[0038] It should be understood that within the scope of the present application, the technical features described in the embodiments herein and the technical features specifically described in the examples can be combined with each other to form a preferred technical solution.

[0039] In this paper, Mettl3 conditional gene knockout mice or Mettl14 conditional gene knockout mice were constructed by applying CRISPR / Cas9 technology, and they were respectively mated with Vasa-cre and Stra8-GFPcre mice specifically expressed in germ cells to obtain reproductive The cell-specific knockout of Mettl3 or Mettl14 mutant mice has been used to construct an animal model of spermatogenesis disorder, which provides new ideas and new methods for the etiological diagnosis, prevention and treatment of human male infertility, as well as the prevention and reduction of paternal birth defects.

[0040] Herein, the animal model is preferably a rodent model.

[0041] Herein, the Mettl3 gene and the...

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Abstract

A Mettl3-knockout spermatogenesis disorder animal model and a building method thereof are provided. The method includes building a Mettl3 conditionally gene-knockout rodent by applying a CRISPR/Cas9 technique; and mating the Mettl3 conditionally gene-knockout rodent and a rodent of the same kind with germ cells capable of specific expression of Cre so as to obtain a Mettl3-knockout spermatogenesisdisorder rodent model with germ cell specificity. The model built provides new ideas and new means for the cause diagnosis, prevention and treatment of human male infertility and prevention and reduction of paternal birth defects.

Description

technical field [0001] The invention relates to a Mettl3 knockout animal model of spermatogenesis disorder and a construction method thereof. Background technique [0002] Infertility, as a major problem that endangers global public health, affects about 10-15% of couples of childbearing age, of which the male factor accounts for 50%. Clinically, non-obstructive azoospermia, oligospermia, asthenospermia and teratospermia are the main manifestations of spermatogenesis disorders; at the same time, spermatogenesis disorders are also one of the important causes of spontaneous abortion and abnormal embryonic development. However, little is currently known about the precise mechanisms underlying abnormalities in spermatogenesis. [0003] The process of spermatogenesis includes three different stages: spermatogonial proliferation, spermatocyte meiosis and sperm metamorphosis. Taking mice as an example, in terms of spermatogonia proliferation, first, a single spermatogonia (A sing...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/85C12N15/90A01K67/027
CPCA01K67/0276C12N9/1007C12N15/8509C12N15/907C12Y201/01062A01K2217/075A01K2227/105A01K2267/03
Inventor 童明汉
Owner CENT FOR EXCELLENCE IN MOLECULAR CELL SCI CHINESE ACAD OF SCI
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