Nucleic acid field rapid-extraction tube and use method thereof

Abstract

The invention provides a nucleic acid field rapid-extraction tube and a use method thereof. The nucleic acid field rapid-extraction tube comprises a push rod, a hollow main tube body and a nucleic acid adsorption device, wherein the push rod is nested in the main tube body; at least one liquid storage chamber is arranged independently of the main body, wherein the liquid storage chamber is communicated with the main tube body; a control valve is arranged to control a communication and / or non-communication state between the liquid storage chamber and the main tube body. The nucleic acid extraction device provided by the invention is simple and portable, can complete a process from tissue sample treatment to rapid extraction and purification of the nucleic acid within 20 to 30 minutes, and is particularly suitable for initially screening a large number of samples rapidly in clinical first-line detection for farms, ports and the like, where epidemics easily occurs, and meanwhile the independently formed liquid storage chamber can avoid cross-infection of reagents or the samples, thus being beneficial to improvement of the purity of nucleic acid.

Description

technical field [0001] The invention relates to the field of molecular biology instruments and equipment, in particular to a nucleic acid on-site rapid extraction tube and a method for using the same. Background technique [0002] Generally speaking, nucleic acid extraction requires multi-step work. First, biological sample materials such as cells and tissue materials need to be crushed, nucleases are inactivated, nucleic acids are released, and other tissue or cell components such as proteins, polysaccharides, and lipids are removed. Thereby obtaining high quality nucleic acid. [0003] Since Swiss physician Friedrich Miescher first successfully extracted DNA from cells in 1869 to the 1990s, nucleic acid extraction has been a tedious, time-consuming task requiring the use of toxic reagents. Until recent years, with the emergence of solid-phase adsorption technology and the development of biochemistry, this problem has been greatly alleviated. However, the rapid extraction...

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Problems solved by technology

[0017] In order to solve the problems existing in the prior art of nucleic acid extraction equipment such as complex structure, easy to cause cross-contamination, long diagnostic cycle, high price, and unsuitable for on-site diagnosis of animal epidemics, on the one hand, the present invention provides a nucleic acid extraction device , including: a push rod, a hollow main body and a nucleic acid adsorption device, wherein the push rod is nested in the main body; at least one liquid storage chamber arranged independently of the main body, wherein the liquid storage chamber communicates with the main body; a control valve, wherein , the control valve is set to control the communication and / or non-communication state between the liquid storage chamber and the main body

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