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A kind of Pseudomonas monsonii and its application

A technology of Pseudomonas monsonii and corn, applied in the direction of application, bacteria, and chemicals for biological control, etc., can solve the problems of delayed, incomplete, and weak corn sowing, and achieve the effect of increasing income and ensuring safety

Active Publication Date: 2021-08-03
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The continuation of the drought in the Northeast in the spring of 2018 led to delays in corn planting, a drop in emergence rate and even dead seedlings, which directly affected the growth and development of corn seedlings
Maize is a crop that is moderately sensitive to soil salinization. Maize has a relatively low salt-alkali tolerance. When subjected to saline-alkali stress, it often fails to germinate normally, resulting in uneven, incomplete, and weak seedlings, which seriously affects the production of maize.

Method used

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  • A kind of Pseudomonas monsonii and its application
  • A kind of Pseudomonas monsonii and its application
  • A kind of Pseudomonas monsonii and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1: Degradation efficiency of Pseudomonas monsonii to residual p-nitrophenol in soil

[0016] (1) Establishment of p-nitrophenol standard working curve:

[0017] Prepare p-nitrophenol standard solutions with concentrations of 200, 100, 50, 25, 12.5, 6.25, 3.125 mg / l, take 400 µl of the prepared p-nitrophenol standard solution, add 2ml of 0.1mol / l sodium hydroxide and 800µl of methanol, after mixing, the absorbance value was measured at 400nm, and drawn into a standard working curve. The standard working curve is y=0.0144x+0.054, and the correlation coefficient is 0.9977, which is used for the research on the degradation efficiency of degrading bacteria on residual p-nitrophenol in soil.

[0018] (2) Preparation of bacterial solution

[0019] Take out the Pseudomonas monsonii stored at -20°C and dissolve it on ice; pipette 100 µl of the bacterial liquid and add it to 20 ml liquid LB medium containing 50 µg / ml kan antibiotics for shaking culture (37°C, 150 rpm...

experiment example 2

[0025] Experimental example 2 Under drought stress, the growth-promoting effect of Pseudomonas monsonii on maize seedlings

[0026] 2.1 Indoor pot experiment

[0027]Mix field soil and peat soil 1:1, weigh 600g per pot. The corn seeds used are Jingke 968. Pick corn seeds with full grains, soak them in 10% NaClO solution for disinfection for 25 minutes, and rinse them with sterile water for 3-5 times. Pour tap water into the flowerpot filled with soil until the soil in the flowerpot is soaked, and record the amount of water required; take 10mL and 30mL of the prepared bacterial solution respectively, add the recorded amount of water and mix well, and then pour it into the filled soil in the flowerpot. Two sterilized corn seeds were sown in each flower pot, and divided into six experimental groups: ①drought, ②drought+10mL bacterial solution, ③dry+30mL bacterial solution, ④normal water supply, ⑤normal water supply+10mL bacterial ⑥Normal water supply + 30mL bacterial solution,...

experiment example 3

[0037] Experimental Example 3 Growth-promoting effect of Pseudomonas monsonii on corn seedlings under sodium bicarbonate stress

[0038] 3.1 Indoor potting experiment: add 600 g of prepared soil (field soil: peat soil = 1:1) to each flower pot, and add different masses of Na 2 CO 3 The aqueous solution simulates alkali stress, making the Na in the soil 2 CO 3 Concentrations were 0, 15, 25 and 40 mmol / kg. Add 15ml OD to the soil 600 =0.1 bacteria solution, 30 days after corn sowing, when the corn seedlings grow to the stage of three leaves and one heart, measure plant height, fresh weight, root length, dry weight and detect chlorophyll content (chlorophyll content analyzer), peroxidase content (more Physiological and biochemical indicators such as cresinol method), superoxide dismutase (nitroblue tetrazolium photochemical reduction method), proline content (ninhydrin method) and malondialdehyde content (thiobarbituric acid method) , with 15 biological replicates for each t...

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Abstract

A kind of Pseudomonas monteilii and its application belong to the technical field of agricultural fertilizers. The Pseudomonas monteilii PN1 screened by the present invention provides a new microbial resource, which has multiple biological functions, It can not only degrade the residual p-nitrophenol in the soil, but also improve the tolerance and stress resistance of corn seedlings to abiotic stresses such as pesticide residues, drought and saline-alkali, which is of great significance for ensuring the safe, green and efficient production of corn and other crops Important guiding significance and application value.

Description

technical field [0001] The invention belongs to the technical field of agricultural fertilizers, in particular to the rhizosphere growth-promoting bacterium Pseudomonas monsonii ( Pseudomonas monteilii )PN1. It can not only degrade the residual p-nitrophenol in the soil, but also be used in improving the drought tolerance and salt-alkali tolerance of corn seedlings. Background technique [0002] Corn is one of the three major food crops in the world, and its output has an important impact on the world's food supply. It is also the most important food, feed and economic crop in China. In the production process of corn, it is often subjected to abiotic stresses such as drought, salinity, cold damage and pesticide residues in the soil, which significantly reduces the yield of corn and brings serious economic losses to agricultural production. Among them, water plays a great role in the life activities of plants. After most plants suffer from drought stress, plant growth and d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B09C1/10A01N63/27A01P21/00C12R1/38
CPCA01N63/10B09C1/10C12N1/205C12R2001/38
Inventor 王岩任何军杨凤慕文洋杨正博
Owner JILIN UNIV
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