43 results about "4-Nitrophenol" patented technology

The invention belongs to the field of soil environment pollution simulation and prediction, and provides a prediction method based on a two-point chemical unbalanced model, which is used for quantitatively evaluating the effect of regulating and controlling the migration behavior of p-nitrophenol in acid red soil by straw stalk biochar. According to the method, a biochar-red soil composite medium system is constructed, a soil column leaching experiment is carried out to obtain penetration curve data, a two-point chemical unbalance model in CXTFIT software is utilized to carry out parameter inversion, and key parameters such as a retardation factor, a two-region distribution coefficient, a mass transfer coefficient and a liquid phase degradation coefficient are accurately identified. Model verification shows that the goodness of fit is high, and a parameter change rule reveals the inherent mechanism of enhancing the pollutant retention capacity, changing the proportion of fast and slow adsorption sites and influencing mass transfer kinetics by the biochar. Based on the verified model, the long-term migration behavior and pollution risk of p-nitrophenol under different environmental conditions can be simulated, and a scientific basis is provided for groundwater pollution control and remediation strategy optimization.

The invention discloses an application of a copper nano-cluster with stable ascorbic acid in detection of p-nitrophenol, the copper nano-cluster with stable ascorbic acid is used as a fluorescent probe, and the detection of p-nitrophenol is realized through a fluorescent sensing method. Due to the quenching effect of p-NP, the fluorescence intensity of the system is obviously reduced in the presence of p-NP. The p-NP concentration can be quantitatively detected by comparing the fluorescence intensity of the copper nano-cluster and the fluorescence intensity of a quenching system. The method is simple, sensitive and high in selectivity, does not need complex fluorescent dye labeling, shows remarkable potential for p-NP detection, and has a good application prospect in the aspects of food, medicine and the like.

The invention discloses an aluminum alloy material easy to oxidize and color and a preparation method thereof.The aluminum alloy material is prepared from, by weight, 20-25 parts of aluminum alloy powder, 5-10 parts of graphene, 4-6 parts of p-nitrophenol, 0.5-1 part of concentrated bromine water and 4-7 parts of magnesium powder. And graphene treatment, aluminum alloy and graphene dispersion treatment, drying, water washing and vacuum hot pressing are carried out. Graphene is doped with the aluminum alloy, the comprehensive performance of the aluminum alloy can be remarkably improved, the hardness can be improved, the electrical conductivity and the thermal conductivity can be remarkably improved, halogen elements are introduced, the activity of the aluminum alloy is improved, the oxidized capacity is improved, the coloring efficiency is improved, and the flame retardant property is improved; and the magnesium powder is added, so that the stability of the system is protected, and the hardness of the system is improved.

The application discloses a fluorescence analysis system for specific response to p-nitrophenol based on gold nanoclusters AuNCs@CFP, 200 muL of gold nanoclusters AuNCs@CFP, 500 muL of PB buffer solution with pH=10 and to-be-detected p-nitrophenol are diluted to 4 mL, the fluorescence intensity of the mixture is determined at an excitation wavelength of 334 nm after reaction at 45 DEG C for 50 min, the concentration of the to-be-detected p-nitrophenol is calculated according to the determined fluorescence intensity and a regression equation, the linear concentration range of the p-nitrophenol is 1.25-40 muM, the regression equation is F0 / F i =0.0258C+1.0003, F0 and F i are the fluorescence intensities of the mixture before and after the addition of the p-nitrophenol respectively, the correlation coefficient R 2 =0.9922, the detection limit LOD is 1.92 muM, the concentration of the p-nitrophenol is 25 muM, the relative standard deviation RSD is 1.13% in 11 repeated determinations. The preparation method of the gold nanoclusters AuNCs@CFP in the application is simple and easy to synthesize, and the fluorescence performance of the gold nanoclusters AuNCs@CFP is excellent.

The invention discloses a texturing process of a single crystal battery piece. The texturing process comprises the following steps: S1, performing pre-texturing treatment on a silicon wafer by adopting pre-texturing liquid; s2, texturing the silicon wafer by adopting texturing liquid; the prefabricated down liquid comprises polyvinylpyrrolidone and polyvinyl alcohol; the texturing liquid comprises inorganic alkali, a defoaming agent and a catalyst; the inorganic base is selected from at least one of sodium hydroxide and potassium hydroxide, and the defoaming agent is selected from at least one of dodecyl trimethyl ammonium chloride, hexadecyl trimethyl ammonium chloride, octadecyl trimethyl ammonium chloride, diethylene glycol, triethylene glycol and tetraethylene glycol. The catalyst is selected from at least one of sodium 5-nitro-o-methoxyphenolate, sodium o-nitrophenolate and sodium p-nitrophenolate. A nucleating agent solution is adopted to carry out texturing pretreatment on a silicon wafer, then alkali treatment texturing is carried out on the silicon wafer, and the liquid changing period of the texturing process is long; and the cell pieces prepared by taking the polyvinylpyrrolidone and the polyvinyl alcohol as the combined nucleating agent are relatively small in inter-piece color difference.

The invention discloses a method for synthesizing mesalazine and application of mesalazine. The method comprises the following steps: introducing p-nitrophenol, formic acid and a Cu-Co-coated MFI-NS molecular sieve catalyst into a continuous flow reaction zone, and sequentially carrying out electrophilic substitution and nitro hydrogenation cascade reaction at normal pressure and 50-70 DEG C; the catalyst has a micropore-mesopore stepped pore channel, and contains a Cu0 metal cluster and a framework Co < 2 + > acid site. And separating, acidifying and crystallizing the reaction liquid to obtain a finished product. According to the invention, the shape-selective selectivity and difunctional synergistic effect of the molecular sieve are utilized, high conversion rate (gt, 98%) and high selectivity (gt, 94%) under mild conditions are realized, the product purity is greater than or equal to 99.5%, and the Cu / Co residue meets the ICHQ3D standard. The catalyst can be recycled for 10 times or more, the yield is reduced by 5% or less, iron mud pollution of a traditional iron powder method and the metal leaching risk of a noble metal catalyst are thoroughly eliminated, and the method has the remarkable advantages of being environmentally friendly, efficient, low in cost, easy to industrially amplify and the like and is suitable for preparing the medicine for treating the inflammatory bowel disease.

The invention relates to the technical field of photocatalysts, in particular to a nonmetal plasmon heterojunction material, a preparation method and application thereof and a method for preparing p-aminophenol through reduction of p-nitrophenol. The nonmetal plasmon heterojunction material provided by the invention comprises a C3N4 nanosheet and a WO3-x nanoplate (x is 0.1 to 0.3) positioned on the surface of the C3N4 nanosheet. The WO3-x local surface plasmon characteristic can effectively expand the spectral response range of the non-metal plasmon heterojunction material in a visible region to a near-infrared region, and the light absorption efficiency of the non-metal plasmon heterojunction material is remarkably enhanced. The nonmetal plasmon heterojunction material provided by the invention can realize efficient reduction of p-nitrophenol based on the synergistic effect of an interface heterojunction, a built-in electric field and oxygen vacancies, is high in selectivity, has excellent photocatalytic activity for reduction of p-nitrophenol, and is excellent in photocatalytic activity stability.

The invention discloses a resin material for 3D printing and a preparation method thereof, and relates to the technical field of photosensitive resin materials. When the resin material for 3D printing is prepared, 3-isocyanate propyl methyl dimethoxy silane is blocked by p-nitrophenol, so that blocked 3-isocyanate propyl methyl dimethoxy silane is prepared; the preparation method comprises the following steps: carrying out condensation on blocked 3-isocyanate group propyl methyl dimethoxy silane, 3-mercaptopropyl methyl dimethoxy silane and hexamethyldisiloxane, so as to prepare blocked isocyanate group mercapto silicone oil; the preparation method comprises the following steps: loading a resorcinol bis (diphenyl phosphate) flame retardant on glass flakes modified by silane to prepare flame-retardant modified glass flakes; the preparation method comprises the following steps: reacting epoxy acrylate, blocked isocyanate sulfydryl silicone oil, acryloyl morpholine and a photoinitiator to prepare photosensitive resin, and compounding the photosensitive resin with flame-retardant modified glass flakes to prepare the resin material for 3D printing. The prepared resin has high mechanical property, flame retardant property and thermal stability.

The invention relates to the technical field of pesticide residue detection, in particular to a soil pesticide residue detection method which comprises the following steps: taking a soil sample to be detected, drying and grinding to obtain soil powder; mixing the soil powder with water, MgSO4 and an acetonitrile solution containing 2.5-3.5% of formic acid, carrying out vortex treatment, ultrasonic treatment and centrifugation, then collecting supernate, repeatedly extracting for one time, and then combining the supernate; and filtering the merged supernate by using a filter membrane, and analyzing by using an ultra-high performance liquid chromatography-triple quadrupole tandem mass spectrometry system. According to the method, extraction and chromatography-mass spectrometry conditions are optimized, 25 pesticide residues such as p-nitrophenol, imidacloprid and metabolites thereof can be detected at the same time, and the method has the advantages of being high in extraction efficiency, easy and convenient to operate, high in matrix interference resistance, high in sensitivity, good in accuracy and the like and is suitable for high-throughput detection and analysis of various pesticide residues in soil.

The application discloses a preparation method of high-purity cephalosporin bipu ester side chain (1a), and a reaction route is shown as follows. The method is characterized in that 2a is used as a starting material to react with triphosgene to generate compound 3a, and then the compound 3a is used to react with p-nitrophenol to generate compound 1a. The product has high purity and high reaction yield, and is suitable for industrial production.

The invention belongs to the technical field of organic matter degradation, and discloses application of a nano-silver catalyst in organic dye catalytic degradation. According to the invention, methylene blue, methyl orange, p-nitroaniline and p-nitrophenol are taken as examples for experiments, and the four dyes start to degrade after reacting for a period of time. In the four dyes, the catalytic effect of methylene blue is worst, and the degradation degree within 20 minutes after the nano-silver catalyst synthesized by biotransformation of the blue-like bacteria FL15 is added can reach the degradation degree of reaction for 6 hours when the catalyst is not added; the effect of catalyzing the degradation of methyl orange is the best, and the degradation rate can reach 95% or above within 10 min; the degradation rate of paranitroaniline and paranitrophenol can reach 95% or above within 20 min. Therefore, the activity of the AgNPs mediated and synthesized by the endophytic fungi FL15 is relatively strong.

The application discloses a preparation and application of a Pt-embedded pudding structure of octahedral TFN-SnO2, and relates to the technical field of nanometer materials. The preparation method comprises the following steps: mixing a niobate aqueous solution, a SnCl2 aqueous solution and hydrofluoric acid, and carrying out hydrothermal treatment to obtain TFN (tin fluoride niobate); centrifuging and washing the TFN, and then dispersing the TFN in water; adding a H2PtCl6 solution to the TFN in water, and continuously stirring the mixture; centrifuging and washing the precipitate to obtain a Pt-TFN / SnO2 composite material. The Pt-embedded pudding structure of octahedral TFN-SnO2 can be obtained without a surfactant. The obtained octahedral Pt-TFN / SnO2 composite material has good catalytic activity in the reaction of reducing nitrophenol into p-aminophenol under alkaline conditions.

The invention provides a preparation method of 4-(3, 3-dichloroallyloxy) phenol, which comprises the following steps: 1) under an alkaline condition, dropwise adding 1, 1, 3-trichloropropene into a first system containing p-nitrophenol, and carrying out etherification reaction to obtain 1-(3, 3-dichloroallyloxy)-4-nitrobenzene; (2) carrying out reduction reaction on the 1-(3, 3-dichloro allyloxy)-4-nitrobenzene, so as to obtain 4-(3, 3-dichloro allyloxy) aniline; (3) carrying out diazotization reaction on the 4-(3, 3-dichloro allyloxy) aniline to obtain 4-(3, 3-dichloro allyloxy) benzene diazonium salt; 4, the 4-(3, 3-dichloro allyloxy) benzene diazonium salt is subjected to a hydrolysis reaction, and the 4-(3, 3-dichloro allyloxy) phenol is obtained.The preparation method of the 4-(3, 3-dichloro allyloxy) phenol is high in reaction selectivity and easy to operate, a solvent is environmentally friendly, and the production cost can be reduced.

The present invention describes the development of a nanometric platform highly sensitive to the presence of two potential water contaminants, hydrazine and p-nitrophenol. This platform is an electrochemical cell containing a graphite mine electrode modified with nanomaterials as the working electrode, a platinum counter electrode, and an Ag / AgCl / Cl (3M) reference electrode. To develop the working electrode for the electrochemical platform, the surface of the graphite electrode was modified with 1-pyrene carboxylic acid and reduced graphene oxide using the droplet deposition technique. Subsequently, the new active surface of the electrode was decorated with molybdenum disulfide (MOS2) nanoroses using the electrodeposition method. The results of the electrochemical study demonstrate the electrocatalytic properties of the nanomaterials used in the platform's development, as well as the strong correlation between current intensity and the concentration of the target analytes. This platform enabled the quantification of the two aforementioned analytes in real water samples with low detection limits of approximately 9.3 and 13.7 nM for hydrazine and p-nitrophenol, respectively.

The application discloses yolk-shell type CuO@MnO2 nano composite microspheres with a multi-point dispersion type inner core and a preparation method thereof. Organic carboxylic acid and copper salt are reacted in water, and after the reaction is completed, the organic copper carboxylate aqueous solution is filtered; after the solvent is removed, the organic copper carboxylate powder solid is obtained; the organic copper carboxylate powder solid is dissolved in a water and ethanol mixed solution; the silicon source ethanol solution is added for reaction; after the reaction is completed, the template agent is separated; the template agent is dispersed in a potassium permanganate solution; the reaction is heated under ultrasonic assistance; after the reaction is completed, the solid is placed in lye and heated to reflux; after centrifugal drying, the yolk-shell type CuO@MnO2 nanometer material with the multi-point dispersion type inner core is obtained by calcination. The yolk-shell type CuO@MnO2 nanometer material with the multi-point dispersion type inner core prepared by the application has the multi-point dispersion type CuO inner core in the MnO2 cavity, and has good application effect when applied to catalytic oxidative degradation of p-nitrophenol.

The present application relates to a kind of p-nitrophenol wastewater treatment device and processing method, the treatment device includes liquid storage device and degradation device, degradation device is equipped with water outlet and water inlet, the position of water outlet is higher than water inlet, water inlet is communicated with liquid storage device by pipeline, p-nitrophenol wastewater is first sent to degradation device from liquid storage device by pipeline, then from water outlet flows back to liquid storage device;Wherein, degradation device inside is equipped with detachable fixed column, fixed column is equipped with detachable iron sheet, the area of iron sheet is less than the cross section of degradation device.When p-nitrophenol in water body is removed using the treatment device, additional catalyst or oxidizing agent is not needed, p-nitrophenol in water body can be completely removed, it is simple and easy to operate and low in cost.

A chemical detoxification method of p-nitrophenol comprises the following steps: S1, adding p-nitrophenol solution, reducing agent and carbon-doped boron nitride non-metal photocatalyst into a quartz reaction tube, stirring uniformly, discharging oxygen and maintaining atmosphere by nitrogen; S2, placing the mixed reaction system into a light reaction instrument, opening a xenon lamp as a light source, opening circulating water, and reacting for 8-60 min at normal temperature; S3, filtering the reaction liquid, and obtaining low-toxicity p-aminophenol compound. The present application uses carbon-doped boron nitride as a non-metal photocatalyst, ethanol or glucose as a reducing agent, and a xenon lamp to simulate sunlight, and reduces p-nitrophenol to p-aminophenol in a nitrogen atmosphere, without using sodium borohydride and metal or noble metal-containing catalysts, and realizing green and efficient detoxification of p-nitrophenol.

A polyacid-based complex for detecting phenolic compounds and trace phenol and application thereof, the complex can be used as a catalyst for detecting trace phenolic compounds by aminoantipyrine colorimetry, and has a molecular formula as follows: {[Cu(dap)(3-PA)]4(SiW 12 O 40 )(H2O)2}·2H2O; wherein, dap is 1,2-propylenediamine; 3-HPA is 3-(3-pyridine) acrylic acid, and 3-PA is a carboxyl group of 3-(3-pyridine) acrylic acid with one proton removed. The polyacid-based complex for detecting phenolic compounds and trace phenol has an application in detecting phenolic compounds. The polyacid-based complex has the advantages of simple synthesis method, short synthesis period and high synthesis yield. The polyacid-based complex can be used to detect various phenolic compounds such as phenol, 4-chlorophenol, o-methylphenol, p-nitrophenol and phloroglucinol, and in particular, the polyacid-based complex can be used to detect trace phenol with low detection limit.

The invention provides a preparation method of an SERS (Surface Enhanced Raman Scattering) substrate based on a gold-silver polyhedron alloy nano frame. The preparation method comprises the following steps: preparing gold polyhedrons with different sizes through a'three-step 'seed growth method; sequentially adding chloroplatinic acid and chloroauric acid to react to form a hollow platinum framework; further adding chloroplatinic acid, silver nitrate and ascorbic acid to prepare a hollow gold-platinum polyhedral nano material; treating by sodium iodide and chloroplatinic acid to obtain a platinum-gold-platinum alloyed nano material, and removing internal gold by using chloroauric acid to form a new platinum framework; selectively depositing gold or silver on the surface to obtain a platinum-gold or platinum-silver alloyed nano material; the gold and silver alloyed nano-frame material is prepared by mixing the gold and silver alloyed nano-frame material and is applied to detection of nitrophenol. The alloy nano-frame can significantly improve the sensitivity and stability of p-nitrophenol detection, and has a good application prospect.

A preparation method of 4, 4 '-dinitrodiphenyl ether belongs to the field of organic synthesis, and comprises the following steps: step 1, weighing p-nitrochlorobenzene, sodium carbonate, a phase transfer catalyst and DMF according to a ratio; 2, mixing p-nitrochlorobenzene, sodium carbonate, a phase transfer catalyst and DMF, and carrying out a reaction; 3, filtering after the reaction is completed, and cooling filtrate to crystallize; 4, after cooling crystallization of the filtrate is completed, filtering and washing; and 5, drying to obtain the product 4, 4 '-dinitrodiphenyl ether. The synthesis method is simple to operate, p-nitrochlorobenzene is converted into 4, 4 '-dinitrodiphenyl ether by adding a proper phase transfer catalyst, sodium p-nitrophenolate is not used any more, the reaction risk is reduced, the reaction steps are shortened, impurities are few, the conversion rate is high, the purity is high, the production efficiency is improved, and the solvent DMF can be recycled and reused.

The application provides a covalent triazine organic framework-cucurbituril composite material and application thereof, and belongs to the technical field of electrochemical detection of environmental pollutants. The specific steps comprise the following steps: synthesizing a CTF material, combining host-guest interaction of cucurbituril [7], and synthesizing a nano cuprous oxide@cucurbituril [7] / covalent triazine organic framework composite material with a cuprite crystal form through a wet chemical reduction precipitation method. The composite material can be used for modifying a glassy carbon electrode to obtain a high-sensitivity electrochemical sensor. The electrochemical sensor constructed in the application has rapid response, high sensitivity and a wide linear range, and is expected to realize on-site detection of p-nitrophenol in water.

The invention discloses preparation of a copper-tin composite oxide and application of the copper-tin composite oxide in catalytic reduction of p-nitrophenol, and relates to the technical field of catalytic materials.The preparation method of the copper-tin composite oxide comprises the following steps that a tinned copper wire is sequentially subjected to acid and alkali treatment, separation, drying and calcination, and the copper-tin composite oxide is obtained. The copper-tin composite oxide prepared by taking the tinned copper wire as the raw material has good catalytic capability, can be used for catalytic reduction of p-nitrophenol, has a good catalytic effect and high catalytic efficiency, and can be used for obtaining a valuable medical intermediate p-aminophenol while treating p-nitrophenol pollution; the copper-tin composite oxide disclosed by the invention has the advantages of low production cost, short preparation process, simple operation process, mild reaction conditions, reusability and the like.

The invention discloses an SMSI-enhanced MnOx / C nano-catalyst, which belongs to the field of nano-catalyst materials, and is specifically prepared by the following steps: reacting potassium permanganate with dopamine hydrochloride in an alkaline solution of tris (hydroxymethyl) aminomethane, and finally performing high-temperature pyrolysis to obtain the SMSI-enhanced MnOx / C nano-catalyst. The SMSI effect is utilized, the non-metal heteroatom doped carrier is promoted to effectively regulate and control the electronic structure of a metal active center through interface interaction, activation of peroxymonosulfate is promoted based on a free radical and non-free radical mixing path, the removal rate of sulfamethoxazole within 30 min is 97.9%, and the removal rate of sulfamethoxazole is 97.9%. The removal efficiency of rhodamine B, bisphenol A, tetracycline hydrochloride and p-nitrophenol is greater than 90.0%, the problem that a single path is limited by organic pollutant types is solved, and the practical application range of the catalyst is widened.

The invention relates to the technical field of biological medicine, in particular to ceramide as well as a synthesis method, a synthesis system and application thereof, the ceramide is mainly applied to cosmetics, a ceramide structure comprises a fatty acid structure and a sphingosine structure, and the sphingosine structure comprises dihydrosphingosine, sphingosine and 6-hydroxy sphingosine; p-nitrophenol ester, the sphingamine structure and an organic solvent are added into a reactor according to the set weight, and the temperature is increased to 30-70 DEG C for a reaction within 1-2 hours; monitoring the completion of the reaction by TLC, concentrating, heating and dissolving with a refining solvent, adding activated carbon, adsorbing at 45-60 DEG C for 1 hour, filtering, cooling the filtrate to 0-10 DEG C, and crystallizing; and recrystallizing by using a refining solvent, and drying to obtain white powdery ceramide. Through the synthesis method, the variety of ceramide raw materials is increased, and a basis is provided for expanding the application of ceramide in downstream markets.

The invention provides a glycosyltransferase high-throughput screening method based on p-nitrophenol, and belongs to the technical field of biology. According to the method, yellow p-nitrophenol disappears after glycosylation is carried out on yellow p-nitrophenol to form glycoside, and the change of absorbance is measured at 400 nm through an ultraviolet-visible spectrophotometer or a microplate reader so as to determine the conversion amount of p-nitrophenol, so that the enzyme activity of glycosyltransferase is measured. Compared with the existing glycosyl transferase activity determination and screening method, the method disclosed by the invention does not need expensive equipment such as liquid chromatography, flow cytometry and the like, and does not need expensive fluorescent substrates, radioactive nucleotide saccharic acid, antibodies and the like. The method has the characteristics of simplicity and convenience in operation, rapidness, high flux and the like.

The application discloses a Fe3O4@Au@CBpExP6 / Ti3C2T X MXene composite photoelectric material, its preparation method and application belong to the field of photoelectrochemistry, Au is decorated on Fe3O4, a layer of water-soluble expanded column [6] arene is wrapped on the surface of Au NPs, and the composite nanoparticles are loaded on Ti3C2T X MXene;Fe3O4@Au@CBpExP6 / Ti3C2T X MXene is used as a photoactive material, a signal on-off type photoelectrochemical PEC biosensing system is designed, and p-nitrophenol is detected;Ti3C2T X MXene has high conductivity, and the recombination probability of electrons and holes is reduced, based on Fe3O4@Au@CBpExP6 / Ti3C2T X MXene has multiple signal amplification capabilities, and the electrode modified by the composite material exhibits excellent performance when detecting p-nitrophenol in the environment.

The invention relates to a high-temperature-resistant biological method for efficiently degrading p-nitrophenol, in particular to application of thermophilic bacteria W-2 to degradation of p-nitrophenol. The strain not only can normally grow in a high-temperature environment of 60 DEG C, but also shows a good degradation effect on p-nitrophenol, and based on the characteristics, the thermophilic bacterium W-2 can be used for degrading p-nitrophenol, and is particularly suitable for treating industrial wastewater containing p-nitrophenol and having a relatively high temperature.

The present application relates to the technical field of refining and purifying p-nitrophenol, and particularly relates to a refining method of high-purity p-nitrophenol, which comprises the following steps: sequentially performing heated acidification and dissolution, heated decoloring treatment on crude p-nitrophenol, then filtering while hot, cooling and crystallizing to obtain a crystalline substance; and using an alcohol solvent to redissolve the crystalline substance, filtering, reducing pressure and concentrating, and drying to obtain p-nitrophenol with a purity higher than 99wt%; the present application develops p-nitrophenol products with higher purity, less impurities and better properties, the purity of the products can be as high as 99.9%, and the products meet the requirements of high-purity; the apparent properties of the products are white crystals, the apparent properties of the products are better, and the products are different from the green-black, brown or brown crystal products on the market; the method is easy to operate, green and environment-friendly, and has strong operability.

The invention belongs to the technical field of metal organic framework material preparation, and provides an oxide-doped metal organic framework material and a preparation method and application thereof.The preparation method comprises the following steps that S1, ferric chloride hexahydrate and 1, 4-terephthalic acid are dissolved in an N, N-dimethylformamide DMF solution, and a precursor solution is formed; s2, adding an oxide into the precursor solution, then performing ultrasonic treatment and stirring to obtain a mixed solution, then pouring the mixed solution into a polytetrafluoroethylene hydrothermal reaction kettle, and putting the polytetrafluoroethylene hydrothermal reaction kettle into a drying oven for crystallization treatment; s3, cooling a crystallized product to room temperature, washing with DMF (Dimethyl Formamide), ethanol and deionized water respectively, centrifuging, and drying the product in a vacuum drying oven to obtain an oxide-doped metal organic framework material; the prepared oxide-doped metal organic framework material can efficiently degrade p-nitrophenol under the condition of normal temperature.

The invention provides a rational design method for improving the hydrolytic activity of lipase, and belongs to the field of enzyme engineering. It is found through molecular simulation that whether the initial conformation of a substrate entering Tunnel is reasonable or not is likely to be an important factor affecting the activity of the substrate. On the basis, key residues at a Tunnel inlet of the lipase are recognized through CaverWeb, NNK degenerate codons are adopted for site-directed saturation mutation to establish a mutant library, and excellent mutants with improved hydrolytic activity are screened by adopting a microwell plate screening method based on a spectrophotometric method. The hydrolytic activity of a plurality of single-region mutants of the candida antarctica lipase B obtained by the invention on 4-p-nitrophenol palmitate at 40 DEG C is improved by more than 10 times compared with that of a wild type; the hydrolysis activity of a plurality of multi-region mutants to pNPP at 40 DEG C is improved by nearly 20 times compared with that of a wild type. A lipase Tunnel inlet is modified, so that the hydrolytic activity of the lipase is greatly improved.