Method for identifying holly germplasm by SSR molecular marker for transcriptome sequencing

A technology of transcriptome sequencing and molecular markers, which is applied in the field of biotechnology, can solve the problems that SSR molecular markers have not been used to identify holly germplasm, and achieve the effects of easy identification and recording, high resolution, and clear main bands

Active Publication Date: 2019-04-19
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0006] At present, there is no method for identifying holly germplasm using SSR molecular markers

Method used

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  • Method for identifying holly germplasm by SSR molecular marker for transcriptome sequencing
  • Method for identifying holly germplasm by SSR molecular marker for transcriptome sequencing
  • Method for identifying holly germplasm by SSR molecular marker for transcriptome sequencing

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Experimental program
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Effect test

Embodiment 1

[0044] 1 material

[0045] 27 holly germplasm resources. The origin comes from China, the United States, and the United Kingdom. The following materials were all planted in the Germplasm Resource Garden of the Institute of Botany, Chinese Academy of Sciences, Jiangsu Province.

[0046]Table 1 The names and main characters of 27 holly test germplasms

[0047]

[0048]

[0049] 1.2 Method

[0050] 1.2.1 Genomic DNA extraction

[0051] The leaves of 27 holly germplasm (Table 1) plants were taken from the nursery of the Institute of Botany, Chinese Academy of Sciences, Jiangsu Province, and DNA was extracted using the Biotech DNA extraction kit. The extracted DNA is tested for DNA concentration and quality by a OneDrop spectrophotometer, and then the DNA is diluted to the desired concentration.

[0052] 1.2.2 Primer design and synthesis

[0053] First, pre-process the sequencing sequence to obtain a high-quality EST sequence. Use MISA software to search for SSR sites i...

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Abstract

The invention discloses a method for identifying holly germplasm by an SSR molecular marker for transcriptome sequencing, and belongs to the field of biotechnology. The method comprises the steps of performing PCR amplification with the SSR molecular marker, wherein the SSR molecular marker is one or more of the following 10 SSR molecular marker primers of IL-1, IL-2, IL-3 , IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9 and IL-10. The method has high polymorphism detection rate and better resolution, and is stable and reliable and simple and efficient.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a set of multiple pairs of SSR marker primers, PCR amplification and its special application to the rapid identification and genetic relationship analysis of Ilex germplasm from the DNA level developed on the basis of Ilex transcriptome sequencing sequences Applications. Background technique [0002] Ilex plants are mostly evergreen broad-leaved tree species, with glossy and dense leathery leaves, beautiful crown, many and dense flowers, bright fruit color, and have high garden ornamental value. In addition, the main ornamental period of holly plants is in autumn and winter, which can greatly make up for the defects of monotonous and depressed winter garden landscape. [0003] China is a country with rich resources of holly plants, about 200 species, which have important economic value and excellent ornamental value, and have huge potential development and application pr...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 陈红李云龙王传永陆小清李乃伟周艳威张凡蔡小龙
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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