Method for identifying holly germplasm by SSR molecular marker for transcriptome sequencing
A technology of transcriptome sequencing and molecular markers, which is applied in the field of biotechnology, can solve the problems that SSR molecular markers have not been used to identify holly germplasm, and achieve the effects of easy identification and recording, high resolution, and clear main bands
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[0044] 1 material
[0045] 27 holly germplasm resources. The origin comes from China, the United States, and the United Kingdom. The following materials were all planted in the Germplasm Resource Garden of the Institute of Botany, Chinese Academy of Sciences, Jiangsu Province.
[0046]Table 1 The names and main characters of 27 holly test germplasms
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[0049] 1.2 Method
[0050] 1.2.1 Genomic DNA extraction
[0051] The leaves of 27 holly germplasm (Table 1) plants were taken from the nursery of the Institute of Botany, Chinese Academy of Sciences, Jiangsu Province, and DNA was extracted using the Biotech DNA extraction kit. The extracted DNA is tested for DNA concentration and quality by a OneDrop spectrophotometer, and then the DNA is diluted to the desired concentration.
[0052] 1.2.2 Primer design and synthesis
[0053] First, pre-process the sequencing sequence to obtain a high-quality EST sequence. Use MISA software to search for SSR sites i...
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