Method for identifying ilex germplasm by utilizing SSR molecular marker

A technology of molecular markers and holly, applied in the field of biotechnology, can solve the problems of germplasm identification of holly plants, difficulty in digging new germplasm of hybrid breeding, stress resistance research, molecular marker development and genetic map construction lag, holly genome and Problems such as the lack of transcriptome data, to achieve the effect of easy identification of records, rich polymorphism, and high sensitivity

Active Publication Date: 2020-07-17
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the genetic background of Ilex plants is not clear, which brings great difficulties to germplasm identification, hybrid breeding, and new germplasm excavation of Ilex plants.
[0004] At the same time, due to the lack of Ilex genome and transcriptome data, the growth and development of Ilex plants, stress resistance research, molecular marker development and genetic map construction are relatively lagging behind, and there are few related reports on the genetic diversity of Ilex plants.
With the development of molecular biology techniques, molecular markers such as RAPD and ISSR have been used for germplasm identification of Ilex plants, but these are mostly dominant markers, which cannot distinguish homozygous and heterozygous

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for identifying ilex germplasm by utilizing SSR molecular marker
  • Method for identifying ilex germplasm by utilizing SSR molecular marker
  • Method for identifying ilex germplasm by utilizing SSR molecular marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] 1 material

[0045] 28 holly germplasm resources. The origin comes from China, the United States, the United Kingdom and other countries. The following materials were all planted in the Germplasm Resource Garden of the Institute of Botany, Chinese Academy of Sciences, Jiangsu Province.

[0046] Table 1 The names and main characters of 28 holly test germplasms

[0047]

[0048]

[0049]

[0050] 1.2 Method

[0051] 1.2.1 Genomic DNA extraction

[0052] The leaves of 28 holly germplasm (Table 1) plants were taken from the nursery of the Institute of Botany, Chinese Academy of Sciences, Jiangsu Province, and DNA was extracted using the Biotech DNA extraction kit. The extracted DNA is tested for DNA concentration and quality by a OneDrop spectrophotometer, and then the DNA is diluted to the desired concentration.

[0053] 1.2.2 Primer design and synthesis

[0054] First, pre-process the sequencing sequence to obtain a high-quality EST sequence. Use MISA softwar...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for identifying ilex germplasm by utilizing an SSR molecular marker, and belongs to the field of biotechnology. The method disclosed by the invention comprises the following steps: carrying out PCR amplification by using the SSR molecular marker, wherein the SSR molecular marker is one or more of the following three SSR molecular marker primers: IL-35, IL-104 and IL-105 separately. The method is high in polymorphism detection rate, better in resolution, stable, reliable, simple and efficient.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a set of multiple pairs of SSR marker primers, PCR amplification and its special application to the rapid identification and genetic relationship analysis of Ilex germplasm from the DNA level developed on the basis of Ilex transcriptome sequencing sequences Applications. Background technique [0002] Ilex plants are mostly evergreen broad-leaved tree species, with glossy and dense leathery leaves, beautiful crown, many and dense flowers, bright fruit color, and have high garden ornamental value. In addition, the main ornamental period of holly plants is in autumn and winter, which can greatly make up for the defects of monotonous and depressed winter garden landscape. [0003] China is a country with rich resources of holly plants, about 200 species, which have important economic value and excellent ornamental value, and have huge potential development and application pr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/156C12Q2531/113C12Q2565/125C12Q2525/151
Inventor 陈红王传永陆小清李云龙周艳威张凡蔡小龙
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap