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Kit capable of quickly diagnosing H1N1 influenza virus

A technology for rapid diagnosis of influenza virus, applied in the direction of microorganism-based methods, measurement/testing of microorganisms, microorganisms, etc., can solve the problems that RCA products cannot be directly observed, and achieve increased detection sensitivity, low cost, simple composition and operation Effect

Inactive Publication Date: 2019-04-23
HUAQIAO UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the detection of RCA products by these dyes cannot be directly observed, and requires the use of equipment for detection

Method used

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  • Kit capable of quickly diagnosing H1N1 influenza virus
  • Kit capable of quickly diagnosing H1N1 influenza virus
  • Kit capable of quickly diagnosing H1N1 influenza virus

Examples

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Embodiment Construction

[0026] A kit for rapid diagnosis of H1N1 influenza virus of the present embodiment includes sequences as shown in SEQ ID No. 1 to SEQ ID No. 7, and also includes an RCA reaction system:

[0027] SEQ ID No. 1:

[0028] 5′-GTACTTCCATTCGACCTCTGTAACAGCCACGAAAACCCTATATCAAAAGTG-3′

[0029] SEQ ID No. 2:

[0030] 5′-CGCAAUGGAAAGAAAUGCUGGAUCUGGUAUUAUCAUUUCAGAUA-3′

[0031] SEQ ID No. 3:

[0032] 5′-UAAUCUGCUAUGACCUGGUGUUGACGGACAGAAUAGUAAUCCCG-3′

[0033] SEQ ID No. 4:

[0034] 5′-CATTTCTTTCCATTGCGTCCTTCATAGATTCTGTCCGTCAACAC-3′

[0035] SEQ ID No. 5:

[0036] 5′-CAGGTCATAGCAGATTAAGAACAGGTAGATAATACAGATCCAG-3′

[0037] SEQ ID No. 6: 5'-GTATCGTCTAATCCTTGTCC-3'

[0038] SEQ ID No. 7: 5'-CCTGCGTTACCTTTCTTTAC-3'

[0039] SEQ ID No. 1 is the nucleic acid aptamer sequence of H1N1 influenza virus, SEQ ID No. 2 to SEQ ID No. 5 are the looping reaction sequences, and SEQ ID No. 6 to SEQ ID No. 7 are the primers of the RCA reaction system.

[0040] Each 50 μL of the RCA reaction system of ...

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Abstract

The invention discloses a kit capable of quickly diagnosing the H1N1 influenza virus. The kit comprises seven nucleotide sequences and a RCA reaction system. When the kit is combined with the H1N1 influenza virus in an aptamer-ligand enrichment sample, rings are specifically formed, two-primer RCA reaction amplification is achieved, the hydroxynaphthol blue of an RCA product visually is developed,and it can be achieved that the H1N1 influenza virus is quickly detected on site.

Description

technical field [0001] The invention belongs to the field of biological detection, in particular to a kit for rapid diagnosis of H1N1 influenza virus. Background technique [0002] H1N1 is an RNA virus belonging to the family Orthomyxoviridae, a type of influenza A virus and one of the most commonly infected influenza viruses in humans. H1N1" is the abbreviation of the name of the virus, the "H" refers to hemagglutinin (Hemagglutinin), and "N" refers to neuraminidase (Neuraminidase), both of which are antigenic names on the virus. It means : A virus with "hemagglutinin type 1, neuraminidase type 1". Its hosts are canines, birds and some mammals. After birds and canines are Transmission and mutation of major mammals, some H1N1 species can be transmitted between humans, including the 1918 influenza outbreak, others can be transmitted between birds and pigs. In March-April 2009, the H1N1 epidemic broke out in Mexico In the early morning of April 30, 2009, the World Health Org...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/6844C12R1/93
CPCC12Q1/6844C12Q1/701C12Q2531/125C12Q2565/113C12Q2525/205
Inventor 李招发汪赛熊莹喆杨会勇林俊生
Owner HUAQIAO UNIVERSITY
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