Silkworm polypeptide function research method and application thereof

A silkworm and functional technology, applied in the field of polypeptide function research, can solve problems such as the limitation of polypeptide types, inaccurate results, and inability to judge the function of polypeptides.

Active Publication Date: 2019-05-24
JIANGSU UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The above method plays an important role in the study of polypeptide functions, but there are many defects. First, the results obtained are not accurate (ex vivo organ research method and homology comparison method), and secondly, the operation process has restrictions on the types of polypeptides. Some polypeptides may It is impossible to study its function in isolated organs. Finally, when conducting gene function research, if the protein precursor encoded by the gene can be cut to form multiple polypeptides, it is impossible to determine which polypeptide plays a role in the physiological process

Method used

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  • Silkworm polypeptide function research method and application thereof
  • Silkworm polypeptide function research method and application thereof
  • Silkworm polypeptide function research method and application thereof

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Embodiment 1

[0034] During the breeding of silkworms, a pigmented mutant purple quail-like spot (purple quail-like, q-l p )(like figure 1 shown), the mutant has two main mutation phenotypes, one is the quail spot (quail,q)-like stripe phenotype, and the body color of the larvae is light pink-purple; the other is that the larvae eat less mulberry and develop Slow, weak constitution, small body, small cocoon. By localization analysis of the mutants, it was found that q-l p The mutated gene of the mutant is a neuropeptide gene Orcokinin. at q-l p In the study, a base deletion occurred in exon 3 of the BmOK gene, and the mutation site was located in the shared exon of the two splice variants BmOKA and BmOKB, resulting in complete loss of the function of the two splice variants. Taking the function research of the above-mentioned polypeptide as an example, the method of the present invention is applied, specifically as follows:

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Abstract

The invention discloses a silkworm polypeptide function research method and application thereof. The method comprises the following steps of knocking out the gene of in-vivo encoded polypeptide firstly and then responding to phenotypic changes emerging due to gene knockout through the living body injection and supplement of the polypeptide via utilizing the characteristic that the polypeptide is carried by body fluid, so that the acting polypeptide is screened out, the functions of the polypeptide are determined as well, and particularly, the method has a very good effect on the polypeptide function research in which a protein precursor can be cut to form multiple kinds of polypeptide.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for researching polypeptide functions, in particular to researching silkworm polypeptide functions by injecting a polypeptide solution into the body cavity of corresponding gene-deficient mutants, and the application of the method in the research of polypeptide functions of other insects. Background technique [0002] Compounds formed by connecting two or more α-amino acids through peptide bonds are called polypeptides. Polypeptides can be synthesized in all cells, and tens of thousands of peptides have been discovered. They are an indispensable and important component of life activities, involving various physiological processes such as hormones, nerves, cell growth, and reproduction. The functions of polypeptides are also multifaceted. Such as: nutritional balance, anti-ulcer, anti-rheumatism, insulin-like effect, lowering blood fat, regulating blood pressure, improving immu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/04
Inventor 赵巧玲王平阳何小柏裘智勇夏定国沈兴家
Owner JIANGSU UNIV OF SCI & TECH
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