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A pumpkin seed extract, a preparation method and applications thereof

A technology for pumpkin seeds and extracts, applied in the field of pumpkin seed extracts, can solve problems such as limited output, and achieve the effects of less side effects and easy materials

Inactive Publication Date: 2019-06-18
CPC CORPORATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to plant growth habits and production areas, the output of most Chinese medicinal materials is limited.

Method used

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  • A pumpkin seed extract, a preparation method and applications thereof
  • A pumpkin seed extract, a preparation method and applications thereof
  • A pumpkin seed extract, a preparation method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Example 1 Preparation of Pumpkin Seed Extract.

[0059] (1) Germination treatment; including washing the shelled pumpkin seeds with water, using alcohol with a volume fraction of 70% to 75% to sterilize the pumpkin seeds, and then heating and soaking the sterilized pumpkin seeds in water, and then soaking them in water Spread the treated pumpkin seeds on top of wet gauze, and place them at room temperature for two days until they germinate; wherein, the heating and soaking treatment includes heating the pumpkin seeds in water at 40°C to 65°C for 20 minutes to 40 minutes, and then soaking them at room temperature Water for 2 hours to 6 hours; it is best to heat in water at 55°C for 30 minutes, and then soak in water at room temperature for 4 hours (operate under this condition in this example). (2) Hull removal and homogenization: Weigh the germinated pumpkin seeds, remove the shells, and pulverize them with a homogenizer to obtain a pumpkin seed powder. (3) Alcohol ext...

Embodiment 2

[0061] Example 2 Composition Analysis of Pumpkin Seed Extract.

[0062] The general pumpkin seed extract extracted according to the steps (2)-(4) of Example 1 without germination treatment and / or plasma treatment was used as the component control group. Take the pumpkin seed extract described in Example 1 or the above-mentioned general pumpkin seed extract, dissolve it in distilled water, and filter it with a filter with a pore size of 0.45 μM to obtain an extract back solution. γ-Aminobutyric acid (γ-Aminobutyric acid, GABA) and tryptophan (Tryptophan) content analysis system uses the method published by Pereira et al. in 2008, after it is moderately modified; Dissolve M GABA in water to prepare GABA standard dilution, or dissolve 0.05M tryptophan in 2.5% KH 2 PO 4 prepared as a tryptophan standard dilution; take 100 μL of the aforementioned extract back into the solution or add 100 μL of the standard dilution to 100 μL of potassium borate solution, and then add 25 μL of o-...

Embodiment 3

[0066] Example 3 Anxiolytic efficacy experiment of pumpkin seed extract.

[0067] The arm-raised plus maze test was used to evaluate the anxiolytic efficacy. The arm-raised plus maze consists of two open arms of 30cm×5cm, two closed arms of 30cm×5cm×15 cm, a 5cm×5cm open square in the middle to connect the arms and feet 50 cm high from the ground composed of racks.

[0068]Experimental procedure: the mice in the experimental group were fed germinated pumpkin seed extract or plasma germinated pumpkin seed extract orally, and the dosage was as described above; the positive control group was orally given 1.5 mg / kg of dinitrogen to the mice flat. After feeding for 60 minutes, the mice were placed in the middle of the cross-arm maze square, one at a time, and measured for 5 minutes, and the cross-arm maze was wiped clean after each mouse was measured.

[0069] Evaluation method of anti-anxiety ability: The time and times that the mice stay in the open arm and the closed arm are ...

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Abstract

The invention aims to provide a pumpkin seed extract, in particular to a pumpkin seed extract extracted from pumpkin seeds subjected to plasma treatment or germination treatment. The invention furtherprovides a preparation method of the pumpkin seed extract. The invention also discloses applications of the pumpkin seed extract in preparation of a composition for resisting depression, anxiety, sedation or sleep assistance.

Description

【Technical field】 [0001] The present invention relates to pumpkin seed extract and its preparation method and use, especially the use of pumpkin seed extract for anti-anxiety, anti-depression, sedation or sleep aid. 【Background technique】 [0002] Mental disorders are diseases in which patients have disorders in behavior, cognition or emotion regulation. With the industrialization and informationization of society, modern people's lives are tense and stressful, and the number of people suffering from mental illness is increasing day by day, making mental illness an important issue that modern people must face. The Diagnostic and Statistical Manual of Mental Disorders V (DSM-5), published by the American Psychiatric Association, divides mental disorders into 20 categories, of which Anxiety disorders and Depressive disorders ) is the most common mental illness. According to the statistics of the World Health Organization, the number of people suffering from anxiety and / or de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/42A61P25/22A61P25/24A61P25/20A23L33/105A61K131/00
Inventor 吴柏龙邱采新彭文煌涂茂园陈锦坤
Owner CPC CORPORATION
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