Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of Bacteroides fragilis in preparation of drugs to induce proliferation and/or accumulation of gamma delta T cells

A technology of Bacteroides fragilis and cell proliferation, applied in the field of immunology research, can solve the problem of unclear immunological function of γδT cells, and achieve the effect of improving immune function

Inactive Publication Date: 2019-06-21
REVAISSANT SHENZHEN BIOSCIENCES CO LTD
View PDF8 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, whether and how B. fragilis affects the immunological function of γδ T cells remains unclear

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of Bacteroides fragilis in preparation of drugs to induce proliferation and/or accumulation of gamma delta T cells
  • Application of Bacteroides fragilis in preparation of drugs to induce proliferation and/or accumulation of gamma delta T cells
  • Application of Bacteroides fragilis in preparation of drugs to induce proliferation and/or accumulation of gamma delta T cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Embodiment 1 Bacteroides fragilis culture

[0053] training method

[0054] Step 1: Take a freeze-dried strain of Bacteroides fragilis (Bacteroides fragilis), add 200 μL of BHI medium, redissolve, absorb 20 μl, draw a line on the blood plate, and put it in the biochemical incubator for 37 degree, anaerobic culture for 48 hours (h);

[0055] Step 2: Pick a single clonal colony and insert it into 10mL BHI medium, and culture it anaerobically at 37°C for 12h;

[0056] Step 3: Take a bottle of 500mL BHI medium, insert 1% (v / v) strains, and culture anaerobically at 37 degrees Celsius (°C) for 48 hours;

[0057] Step 4: Take the bacterial solution and centrifuge at 6000rpm for 10min. Wash twice with normal saline, and finally reconstitute the sludge with normal saline for use and count the viable bacteria.

Embodiment 2

[0058] Example 2 Bacteroides fragilis induces the expression of γδTCR, the proliferation and accumulation of γδT cells and the enhancement of effector function in mice immunized with Mycobacterium tuberculosis

[0059] 1. Training method

[0060] The culture method of Bacteroides fragilis is the same as in Example 1.

[0061] 2. Sample preparation

[0062] 1) Preparation of live Bacteroides fragilis ZY-312 cells

[0063] training method

[0064] Step 1: Take a freeze-dried strain of Bacteroides fragilis (Bacteroides fragilis), add 200 μL of BHI medium, redissolve, absorb 20 μl, draw a line on the blood plate, and put it in the biochemical incubator for 37 degree, anaerobic culture for 48h;

[0065] Step 2: Pick a monoclonal colony and insert it into 10mL of BHI medium, and culture it anaerobically at 37°C for 12 hours;

[0066] Step 3: Take a bottle of 500mL BHI medium, insert 1% (v / v) strains, and incubate anaerobically at 37 degrees Celsius for 48 hours;

[0067] Step ...

Embodiment 3

[0077] Example 3 Experiment of Bacteroides fragilis Inducing γδTCR Expression, γδT Cell Proliferation, Accumulation and Effector Function Enhancement in Melanoma Transplanted Mice

[0078] 1. Training method

[0079] The culture method of Bacteroides fragilis is the same as in Example 1.

[0080] 2. Sample preparation

[0081] Sample preparation method is the same as embodiment 2

[0082] 3. Bacteroides fragilis induces the expression of γδT and the enhancement of effector functions in melanoma transplanted mice figure 2 Schematic diagram of the experimental flow for detecting that B. fragilis enhances the proliferation and accumulation of γδT cells or promotes their effector functions in tumors.

[0083] Experimental animals: 36 3- to 4-week-old C57BL / 6 mice, in good mental state, were purchased from the Experimental Animal Center of Sun Yat-sen University. The mice were randomly divided into 3 groups, 12 in each group, and the 3 groups were respectively the control grou...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the field of biomedicine, provides application of Bacteroides fragilis in the preparation of drugs to induce proliferation and / or accumulation of gamma delta T cells, and particularly relates to application of Bacteroides fragilis in the preparation of drugs to enhance functionality of gamma delta T cells.

Description

technical field [0001] The invention belongs to the field of immunology research, and the invention relates to a composition capable of inducing the proliferation, accumulation and stronger effector functions of γδ T cells (especially TCRγδ strong positive T cells) in infectious diseases and / or tumors, and the combination The product contains Bacteroides fragilis or a physiologically active substance obtained from Bacteroides fragilis, or a composition using Bacteroides fragilis, etc., as an active ingredient. The present invention also relates to methods for inducing the proliferation of γδT cells and enhancing their effector functions in infectious diseases and / or tumors. Background technique [0002] Hundreds of commensal microbial species inhabit the mammalian gastrointestinal tract and closely interact with the host immune system. Results of studies using germ-free (GF) animals have shown that commensal microorganisms exert a strong influence on the formation of the mu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/741A61P37/04A61P35/00A61P31/06A61P31/04A61P31/10A61P31/12A23L33/135
CPCA23L33/135A61K35/741A61P31/04A61P31/06A61P31/10A61P31/12A61P35/00A61P37/04C12N1/20C12N1/36A23V2002/00A61K2035/115
Inventor 曾谷城
Owner REVAISSANT SHENZHEN BIOSCIENCES CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com