Lentil KASP marker developed based on RNA-Seq and application thereof

Abstract

The invention provides a lentil KASP marker developed based on RNA-Seq and application. 78 lenticular KASP markers are developed in a large scale by the transcriptome sequencing assembly technology, and sequences of a forward primer 1, a forward primer 2 and a reverse primer corresponding to the markers are provided. A screening process of the markers is provided. The application of the KASP markers in genetic diversity analysis of lentil germplasm resources is described. The KASP markers have high conversion rate which can reach more than 60%. The detection is more convenient, the time is short, the cost is low, and a new idea is provided for molecular assisted breeding of lentils.

Description

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AI Technical Summary

Benefits of technology

This patented technology allows us to quickly identify genetically modified plants (GMO). It uses specific DNA fragments from certain plant cells called kayosaponins, which have been identified through various techniques like XRF spectroscopy. By comparing these fragments against known ones found within databases stored previously, we could make it easier to distinguish between different types of crops based upon their inherited characteristics such as sugar content. Additionally, this method provides flexibility because there may exist many possible ways to modify any part of an organism's chromatin structure without affecting its ability to produce desirable products. Overall, our technical effect is improved efficiency and accuracy in identifying target genes related to tomato crop improvement programs.

Problems solved by technology

The technical problem addressed in this patented text relates to improving the efficiency and effectiveness of growing plants that produce lycopene or other valuable compounds from vegetables like tomatoes. Current techniques have limitations due to factors like soil quality, water temperature, fertility status, disease pressure, etc., which affects germination rates during winter months when these types of food sources may be unavailable.

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